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While isolated examples of <jats:italic>trans-<\/jats:italic> changes have been identified, the scale of their overall contribution to regulatory and phenotypic evolution remains unclear.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Here, we attempt to examine the prevalence of <jats:italic>trans-<\/jats:italic> effects and their potential impact on gene expression patterns in vertebrate evolution by comparing the function of identical human tissue-specific enhancer sequences in two highly divergent vertebrate model systems, mouse and zebrafish. Among 47 human conserved non-coding elements (CNEs) tested in transgenic mouse embryos and in stable zebrafish lines, at least one species-specific expression domain was observed in the majority (83%) of cases, and 36% presented dramatically different expression patterns between the two species. Although some of these discrepancies may be due to the use of different transgenesis systems in mouse and zebrafish, in some instances we found an association between differences in enhancer activity and changes in the endogenous gene expression patterns between mouse and zebrafish, suggesting a potential role for <jats:italic>trans-<\/jats:italic> changes in the evolution of gene expression.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>In total, our results: (i) serve as a cautionary tale for studies investigating the role of human enhancers in different model organisms, and (ii) suggest that changes in the <jats:italic>trans<\/jats:italic> environment may play a significant role in the evolution of gene expression in vertebrates.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-13-713","type":"journal-article","created":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T02:45:11Z","timestamp":1355971511000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Differences in enhancer activity in mouse and zebrafish reporter assays are often associated with changes in gene expression"],"prefix":"10.1186","volume":"13","author":[{"given":"Ana","family":"Ariza-Cosano","sequence":"first","affiliation":[]},{"given":"Axel","family":"Visel","sequence":"additional","affiliation":[]},{"given":"Len A","family":"Pennacchio","sequence":"additional","affiliation":[]},{"given":"Hunter B","family":"Fraser","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Luis","family":"G\u00f3mez-Skarmeta","sequence":"additional","affiliation":[]},{"given":"Manuel","family":"Irimia","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9","family":"Bessa","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2012,12,19]]},"reference":[{"key":"4596_CR1","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1126\/science.1090005","volume":"188","author":"MC King","year":"1975","unstructured":"King MC, Wilson AC: Evolution at two levels in humans and chimpanzees. 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