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We subsequently confirmed this result using sucrose gradient ribosome profiling joined with western blot analysis. We found that RNase R co-migrates with the single 30S ribosomal subunits. Independent data involving RNase R in the rRNA quality control process allowed us to hypothesize that the RNase R connection with ribosomes has an important physiological role.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This study leads us to conclude that RNase R can interact with ribosomal proteins and that this interaction may be a result of this enzyme involvement in the ribosome quality control.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-14-34","type":"journal-article","created":{"date-parts":[[2014,2,11]],"date-time":"2014-02-11T18:01:48Z","timestamp":1392141708000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of the RNase R association with ribosomes"],"prefix":"10.1186","volume":"14","author":[{"given":"Michal","family":"Malecki","sequence":"first","affiliation":[]},{"given":"C\u00e1tia","family":"B\u00e1rria","sequence":"additional","affiliation":[]},{"given":"Cecilia M","family":"Arraiano","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2014,2,11]]},"reference":[{"key":"2213_CR1","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/S0079-6603(08)00805-2","volume":"85","author":"JM Andrade","year":"2009","unstructured":"Andrade JM, Pobre V, Silva IJ, Domingues S, Arraiano CM: The role of 3\u2032-5\u2032 exoribonucleases in RNA degradation. 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