{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T10:54:12Z","timestamp":1766400852351},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Staphylococcus aureus<\/jats:italic> immunodominant surface antigen B (IsaB) elicits an immune response during septicemia and is generally classified as a virulence factor, but its biological function remains completely undefined. In an attempt to identify staphylococcal RNA-binding proteins, we designed an RNA Affinity Chromatography assay and subsequently isolated IsaB.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Western analysis indicated that IsaB was both secreted and cell-surface associated. Gel Shift analysis confirmed the RNA binding activity but revealed that IsaB bound to any nucleic acid without sequence specificity. IsaB exhibited the highest affinity for double-stranded DNA followed by single-stranded DNA and RNA. Because extracellular DNA has been shown to play a role in biofilm formation, we investigated the biofilm-forming capacity of an isogenic <jats:italic>isaB<\/jats:italic> deletion mutant but we found that IsaB did not contribute to biofilm formation under any conditions tested.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>IsaB is an extracellular nucleic acid binding protein, with little to no sequence specificity, but its role in virulence remains unclear.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-9-61","type":"journal-article","created":{"date-parts":[[2009,3,27]],"date-time":"2009-03-27T07:13:49Z","timestamp":1238138029000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein"],"prefix":"10.1186","volume":"9","author":[{"given":"Nicole M","family":"Mackey-Lawrence","sequence":"first","affiliation":[]},{"given":"Denise E","family":"Potter","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Cerca","sequence":"additional","affiliation":[]},{"given":"Kimberly K","family":"Jefferson","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,3,26]]},"reference":[{"issue":"Suppl 5","key":"732_CR1","doi-asserted-by":"publisher","first-page":"S350","DOI":"10.1086\/533591","volume":"46","author":"RJ Gordon","year":"2008","unstructured":"Gordon RJ, Lowy FD: Pathogenesis of methicillin-resistant Staphylococcus aureus infection. 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