{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T11:37:08Z","timestamp":1768822628693,"version":"3.49.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2007,7,23]],"date-time":"2007-07-23T00:00:00Z","timestamp":1185148800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Cancer"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Aberrant promoter hypermethylation of cancer-associated genes occurs frequently during carcinogenesis and may serve as a cancer biomarker. In this study we aimed at defining a quantitative gene promoter methylation panel that might identify the most prevalent types of renal cell tumors.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>A panel of 18 gene promoters was assessed by quantitative methylation-specific PCR (QMSP) in 85 primarily resected renal tumors representing the four major histologic subtypes (52 clear cell (ccRCC), 13 papillary (pRCC), 10 chromophobe (chRCC), and 10 oncocytomas) and 62 paired normal tissue samples. After genomic DNA isolation and sodium bisulfite modification, methylation levels were determined and correlated with standard clinicopathological parameters.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Significant differences in methylation levels among the four subtypes of renal tumors were found for <jats:italic>CDH1<\/jats:italic> (<jats:italic>p<\/jats:italic> = 0.0007), <jats:italic>PTGS2<\/jats:italic> (<jats:italic>p<\/jats:italic> = 0.002), and <jats:italic>RASSF1A<\/jats:italic> (<jats:italic>p<\/jats:italic> = 0.0001). <jats:italic>CDH1<\/jats:italic> hypermethylation levels were significantly higher in ccRCC compared to chRCC and oncocytoma (<jats:italic>p<\/jats:italic> = 0.00016 and <jats:italic>p<\/jats:italic> = 0.0034, respectively), whereas <jats:italic>PTGS2<\/jats:italic> methylation levels were significantly higher in ccRCC compared to pRCC (<jats:italic>p<\/jats:italic> = 0.004). <jats:italic>RASSF1A<\/jats:italic> methylation levels were significantly higher in pRCC than in normal tissue (<jats:italic>p<\/jats:italic> = 0.035). In pRCC, <jats:italic>CDH1<\/jats:italic> and <jats:italic>RASSF1A<\/jats:italic> methylation levels were inversely correlated with tumor stage (<jats:italic>p<\/jats:italic> = 0.031) and nuclear grade (<jats:italic>p<\/jats:italic> = 0.022), respectively.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The major subtypes of renal epithelial neoplasms display differential aberrant <jats:italic>CDH1<\/jats:italic>, <jats:italic>PTGS2<\/jats:italic>, and <jats:italic>RASSF1A<\/jats:italic> promoter methylation levels. This gene panel might contribute to a more accurate discrimination among common renal tumors, improving preoperative assessment and therapeutic decision-making in patients harboring suspicious renal masses.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2407-7-133","type":"journal-article","created":{"date-parts":[[2007,7,24]],"date-time":"2007-07-24T06:13:59Z","timestamp":1185257639000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Quantitative promoter methylation analysis of multiple cancer-related genes in renal cell tumors"],"prefix":"10.1186","volume":"7","author":[{"given":"Vera L","family":"Costa","sequence":"first","affiliation":[]},{"given":"Rui","family":"Henrique","sequence":"additional","affiliation":[]},{"given":"Franclim R","family":"Ribeiro","sequence":"additional","affiliation":[]},{"given":"Mafalda","family":"Pinto","sequence":"additional","affiliation":[]},{"given":"Jorge","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Francisco","family":"Lobo","sequence":"additional","affiliation":[]},{"given":"Manuel R","family":"Teixeira","sequence":"additional","affiliation":[]},{"given":"Carmen","family":"Jer\u00f3nimo","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2007,7,23]]},"reference":[{"key":"783_CR1","unstructured":"Globocan database. 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