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Here we describe the application of phage \u03d5IBB-PF7, a newly isolated phage, to control<jats:italic>P. fluorescens<\/jats:italic>biofilms. The biofilms were formed under static or dynamic conditions and with or without renewal of medium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Conditions for biofilm formation influenced the feature of the biofilm and the morphology of<jats:italic>P. fluorescens<\/jats:italic>. Biomass removal due to phage activity varied between 63 and 91% depending on the biofilm age and the conditions under which the biofilm had been formed and phages applied. Removal of the biofilm by phage treatment was faster in younger biofilms, but the same number of surviving cells was detected in all tested biofilms, after only 4 h of treatment, even in older biofilms. Under static conditions, a 3 log higher number of phage progeny remained either inside the biofilm matrix or attached to the substratum surface than under dynamic conditions, pointing to the importance of experimental conditions for the efficacy of phage entrapment into the biofilm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Phage \u03d5IBB-PF7A is highly efficient in removing<jats:italic>P. fluorescens<\/jats:italic>biofilms within a short time interval. The conditions of biofilm formation and applied during phage infection are critical for the efficacy of the sanitation process. The integration of phages into the biofilm matrix and their entrapment to the surface may be further beneficial factors when phage treatment is considered alone or in addition to chemical biocides in industrial environments where<jats:italic>P. fluorescens<\/jats:italic>causes serious spoilage.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1472-6750-8-79","type":"journal-article","created":{"date-parts":[[2008,10,28]],"date-time":"2008-10-28T07:15:02Z","timestamp":1225178102000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":111,"title":["Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7A"],"prefix":"10.1186","volume":"8","author":[{"given":"Sanna","family":"Sillankorva","sequence":"first","affiliation":[]},{"given":"Peter","family":"Neubauer","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Azeredo","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2008,10,27]]},"reference":[{"key":"348_CR1","first-page":"37","volume":"40","author":"RE Chandler","year":"1985","unstructured":"Chandler RE, Mcmeekin TA: Temperature Function Integration and Its Relationship to the Spoilage of Pasteurized, Homogenized Milk. 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