{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:58:47Z","timestamp":1772585927375,"version":"3.50.1"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Biotechnol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Despite the proven relevance of<jats:italic>Pseudomonas fluorescens<\/jats:italic>as a spoilage microorganism in milk, fresh meats and refrigerated food products and the recognized potential of bacteriophages as sanitation agents, so far no phages specific for<jats:italic>P. fluorescens<\/jats:italic>isolates from dairy industry have been closely characterized in view of their lytic efficiency. Here we describe the isolation and characterization of a lytic phage capable to infect a variety of<jats:italic>P. fluorescens<\/jats:italic>strains isolated from Portuguese and United States dairy industries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several phages were isolated which showed a different host spectrum and efficiency of lysis. One of the phages, phage \u03d5IBB-PF7A, was studied in detail due to its efficient lysis of a wide spectrum of<jats:italic>P. fluorescens<\/jats:italic>strains and ribotypes. Phage \u03d5IBB-PF7A with a head diameter of about 63 nm and a tail size of about 13 \u00d7 8 nm belongs morphologically to the<jats:italic>Podoviridae<\/jats:italic>family and resembles a typical T7-like phage, as analyzed by transmission electron microscopy (TEM). The phage growth cycle with a detected latent period of 15 min, an eclipse period of 10 min, a burst size of 153 plaque forming units per infected cell, its genome size of approximately 42 kbp, and the size and N-terminal sequence of one of the protein bands, which gave similarity to the major capsid protein 10A, are consistent with this classification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The isolated T7-like phage, phage \u03d5IBB-PF7A, is fast and efficient in lysing different<jats:italic>P. fluorescens<\/jats:italic>strains and may be a good candidate to be used as a sanitation agent to control the prevalence of spoilage causing<jats:italic>P. fluorescens<\/jats:italic>strains in dairy and food related environments.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1472-6750-8-80","type":"journal-article","created":{"date-parts":[[2008,10,28]],"date-time":"2008-10-28T07:15:02Z","timestamp":1225178102000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens"],"prefix":"10.1186","volume":"8","author":[{"given":"Sanna","family":"Sillankorva","sequence":"first","affiliation":[]},{"given":"Peter","family":"Neubauer","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Azeredo","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2008,10,27]]},"reference":[{"key":"349_CR1","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S0168-1605(99)00038-0","volume":"48","author":"I Arnaut-Rollier","year":"1999","unstructured":"Arnaut-Rollier I, De Zutter L, Van Hoof J: Identities of the Pseudomonas spp. in flora from chilled chicken. 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