{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:38:05Z","timestamp":1777426685264,"version":"3.51.4"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Malar J"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>A reliable and simple test for the detection of malaria parasite is crucial in providing effective treatment and therapeutic follow-up, especially in malaria elimination programmes. A comparison of four methods, including nested polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) were used for the malaria diagnosis and treatment follow-up in S\u00e3o Tom\u00e9 and Pr\u00edncipe, during a successful pre-elimination campaign.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Method<\/jats:title>\n            <jats:p>During the period September to November 2009, blood samples from 128 children (five to 14 years old) with temperature \u226538\u00b0C (tympanic) in the District of Agua Grande were examined using four different methods, i.e., histidine-rich protein 2 (HRP-2) based rapid diagnostic tests (HRP-2-RDTs), optical microscopy, nested PCR, and LAMP. First-line treatment with artesunate-amodiaquine was given for uncomplicated malaria and intravenous quinine was given for complicated malaria. Children with persistent positivity for malaria by microscopy, or either by nested PCR, or by LAMP on day 7 were given second-line treatment with artemether-lumefantrine. Treatment follow-up was made weekly, for up to four weeks.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>On day 0, positive results for HRP-2-RDTs, microscopy, nested PCR, and LAMP, were 68(53%), 47(37%), 64(50%), and 65(51%), respectively. When nested PCR was used as a reference standard, only LAMP was comparable; both HRP-2-RDTs and microscopy had moderate sensitivity; HRP-2-RDTs had poor positive predictive value (PPV) and a moderate negative predictive value (NPV) for the treatment follow-up. Seventy-one children with uncomplicated malaria and eight children with complicated falciparum malaria were diagnosed based on at least one positive result from the four tests as well as clinical criteria. Twelve of the 79 children receiving first-line treatment had positive results by nested PCR on day 7 (nested PCR-corrected day 7 cure rate was 85%). After the second-line treatment, nested PCR\/LAMP-corrected day 28 cure rate was 83% for these 12 children.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>HRP-2-RDTs have similar sensitivity as microscopy but less specificity. However, as compared to nested PCR, the poor sensitivity of HRP-2-RDTs indicates that low parasitaemia may not be detected after treatment, as well as the low specificity of HRP-2-RDTs indicates it cannot be applied for treatment follow-up. LAMP has similar sensitivity and specificity to nested PCR. With high PPV and NPV, LAMP is simpler and faster as compared to nested PCR with the advantage of detecting low parasitaemia becoming a potential point-of-care test for treatment follow-up.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1475-2875-11-408","type":"journal-article","created":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T15:26:05Z","timestamp":1354807565000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in S\u00e3o Tom\u00e9"],"prefix":"10.1186","volume":"11","author":[{"given":"Pei-Wen","family":"Lee","sequence":"first","affiliation":[]},{"given":"Dar-Der","family":"Ji","sequence":"additional","affiliation":[]},{"given":"Chia-Tai","family":"Liu","sequence":"additional","affiliation":[]},{"given":"Herodes S","family":"Rampao","sequence":"additional","affiliation":[]},{"given":"Virgilio E","family":"do Rosario","sequence":"additional","affiliation":[]},{"given":"I-Feng","family":"Lin","sequence":"additional","affiliation":[]},{"given":"Men-Fang","family":"Shaio","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2012,12,6]]},"reference":[{"key":"2560_CR1","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/1475-2875-9-26","volume":"9","author":"PW Lee","year":"2010","unstructured":"Lee PW, Liu CT, Rampao HS, do Rosario VE, Shaio MF: Pre-elimination of malaria on the island of Pr\u00edncipe. Malar J. 2010, 9: 26-10.1186\/1475-2875-9-26.","journal-title":"Malar J"},{"key":"2560_CR2","volume-title":"World malaria report","author":"World Health Organization","year":"2011","unstructured":"World Health Organization: World malaria report. 2011, Geneva: World Health Organization"},{"key":"2560_CR3","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1186\/1475-2875-9-264","volume":"9","author":"PW Lee","year":"2010","unstructured":"Lee PW, Liu CT, do Rosario VE, de Sousa B, Rampao HS, Shaio MF: Potential threat of malaria epidemics in a low transmission area, as exemplified by Sao Tome and Principe. Malar J. 2010, 9: 264-10.1186\/1475-2875-9-264.","journal-title":"Malar J"},{"key":"2560_CR4","doi-asserted-by":"crossref","first-page":"523","DOI":"10.4269\/ajtmh.2001.65.523","volume":"65","author":"L Von Seidlein","year":"2001","unstructured":"Von Seidlein L, Drakeley C, Greenwood B, Walraven G, Targett G: Risk factors for gametocyte carriage in Gambian children. Am J Trop Med Hyg. 2001, 65: 523-527.","journal-title":"Am J Trop Med Hyg"},{"key":"2560_CR5","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1111\/j.1365-3156.2004.01277.x","volume":"9","author":"D Njama-Meya","year":"2004","unstructured":"Njama-Meya D, Kamya MR, Dorsey G: Asymptomatic parasitaemia as a risk factor for symptomatic malaria in a cohort of Ugandan children. Trop Med Int Health. 2004, 9: 862-868. 10.1111\/j.1365-3156.2004.01277.x.","journal-title":"Trop Med Int Health"},{"key":"2560_CR6","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/1475-2875-3-18","volume":"3","author":"JT Bousema","year":"2004","unstructured":"Bousema JT, Gouagna LC, Drakeley CJ, Meutstege AM, Okech BA, Akim IN, Beier JC, Githure JI, Sauerwein RW: Plasmodium falciparum gametocyte carriage in asymptomatic children in western Kenya. Malar J. 2004, 3: 18-10.1186\/1475-2875-3-18.","journal-title":"Malar J"},{"key":"2560_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1475-2875-11-29","volume":"11","author":"DD Laishram","year":"2012","unstructured":"Laishram DD, Sutton PL, Nanda N, Sharma VL, Sobti RC, Carlton JM, Joshi H: The complexities of malaria disease manifestations with a focus on asymptomatic malaria. Malar J. 2012, 11: 1-10.1186\/1475-2875-11-1. 29","journal-title":"Malar J"},{"key":"2560_CR8","doi-asserted-by":"publisher","first-page":"e20805","DOI":"10.1371\/journal.pone.0020805","volume":"6","author":"S Karl","year":"2011","unstructured":"Karl S, Gurarie D, Zimmerman PA, King CH, St Pierre TG, Davis TME: A sub-microscopic gametocyte reservoir can sustain malaria transmission. PLoS One. 2011, 6: e20805-10.1371\/journal.pone.0020805.","journal-title":"PLoS One"},{"issue":"suppl","key":"2560_CR9","doi-asserted-by":"publisher","first-page":"S5","DOI":"10.1186\/1475-2875-7-S1-S5","volume":"7","author":"MD Perkins","year":"2008","unstructured":"Perkins MD, Bell DR: Working without a blindfold: the critical role of diagnostics in malaria control. Malar J. 2008, 7 (suppl): S5-10.1186\/1475-2875-7-S1-S5.","journal-title":"Malar J"},{"key":"2560_CR10","doi-asserted-by":"publisher","first-page":"e1000063","DOI":"10.1371\/journal.pmed.1000063","volume":"6","author":"Z Bisoffi","year":"2009","unstructured":"Bisoffi Z, Gobbi F, Angheben A, den Ende JV: The role of rapid diagnostic tests in managing malaria. PLoS Med. 2009, 6: e1000063-10.1371\/journal.pmed.1000063.","journal-title":"PLoS Med"},{"key":"2560_CR11","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1136\/jcp.47.8.740","volume":"47","author":"LM Milne","year":"1994","unstructured":"Milne LM, Kyi MS, Chiodini PL, Warhurst DC: Accuracy of routine laboratory diagnosis of malaria in the United Kingdom. J Clin Pathol. 1994, 47: 740-742. 10.1136\/jcp.47.8.740.","journal-title":"J Clin Pathol"},{"key":"2560_CR12","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1136\/jcp.49.7.533","volume":"49","author":"DC Warhurst","year":"1996","unstructured":"Warhurst DC, Williams JE: Laboratory diagnosis of malaria. J Clin Pathol. 1996, 49: 533-538. 10.1136\/jcp.49.7.533.","journal-title":"J Clin Pathol"},{"key":"2560_CR13","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1128\/CMR.15.1.66-78.2002","volume":"15","author":"A Moody","year":"2002","unstructured":"Moody A: Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev. 2002, 15: 66-78. 10.1128\/CMR.15.1.66-78.2002.","journal-title":"Clin Microbiol Rev"},{"key":"2560_CR14","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1186\/1475-2875-8-211","volume":"8","author":"S Houze","year":"2009","unstructured":"Houze S, Boly MD, Le Bras J, Deloron P, Faucher JF: PfHRP2 and PfLDH antigen detection for monitoring the efficacy of artemisinin-based combination therapy (ACT) in the treatment of uncomplicated falciparum malaria. Malar J. 2009, 8: 211-10.1186\/1475-2875-8-211.","journal-title":"Malar J"},{"key":"2560_CR15","doi-asserted-by":"publisher","first-page":"e40633","DOI":"10.1371\/journal.pone.0040633","volume":"7","author":"L Goncalves","year":"2012","unstructured":"Goncalves L, Subtil A, de Oliveira R, do Rosario V, Lee PW, Shaio MF: Bayesian latent class models in malaria diagnosis. PLoS One. 2012, 7: e40633-10.1371\/journal.pone.0040633.","journal-title":"PLoS One"},{"key":"2560_CR16","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1016\/S1471-4922(02)02348-6","volume":"18","author":"T H\u00e4nscheida","year":"2002","unstructured":"H\u00e4nscheida T, Grobusch MP: How useful is PCR in the diagnosis of malaria?. Trends Parasitol. 2002, 18: 395-398. 10.1016\/S1471-4922(02)02348-6.","journal-title":"Trends Parasitol"},{"key":"2560_CR17","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1373\/clinchem.2005.057901","volume":"52","author":"LL Poon","year":"2006","unstructured":"Poon LL, Wong BW, Ma EH, Chan KH, Chow LM, Abeyewickreme W, Tangpukdee N, Yuen KY, Guan Y, Looareesuwan S, Peiris JS: Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. 2006, 52: 303-306.","journal-title":"Clin Chem"},{"key":"2560_CR18","doi-asserted-by":"publisher","first-page":"2521","DOI":"10.1128\/JCM.02117-06","volume":"45","author":"ET Han","year":"2007","unstructured":"Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T: Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol. 2007, 45: 2521-2528. 10.1128\/JCM.02117-06.","journal-title":"J Clin Microbiol"},{"key":"2560_CR19","doi-asserted-by":"publisher","first-page":"e13733","DOI":"10.1371\/journal.pone.0013733","volume":"5","author":"NW Lucchi","year":"2010","unstructured":"Lucchi NW, Demas A, Narayanan J, Sumari D, Kabanywanyi A, Kachur SP, Barnwell JW, Udhayakumar V: Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malaria. PLoS One. 2010, 5: e13733-10.1371\/journal.pone.0013733.","journal-title":"PLoS One"},{"key":"2560_CR20","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/0166-6851(93)90050-8","volume":"58","author":"G Snounou","year":"1993","unstructured":"Snounou G, Viriyakosol S, Jarra W, Thaithong S, Brown KN: Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol. 1993, 58: 283-292. 10.1016\/0166-6851(93)90050-8.","journal-title":"Mol Biochem Parasitol"},{"key":"2560_CR21","doi-asserted-by":"crossref","first-page":"249","DOI":"10.4269\/ajtmh.2005.72.249","volume":"72","author":"S Bereczky","year":"2005","unstructured":"Bereczky S, M\u00e5rtensson A, Gil JP, F\u00e4rnert A: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum. Am J Trop Med Hyg. 2005, 72: 249-251.","journal-title":"Am J Trop Med Hyg"},{"key":"2560_CR22","doi-asserted-by":"publisher","first-page":"1892","DOI":"10.1128\/JCM.00105-09","volume":"47","author":"SY Liang","year":"2009","unstructured":"Liang SY, Chan YH, Hsia KT, Lee JL, Kuo MC, Hwa KY, Chan CW, Chiang TY, Chen JS, Wu FT, Ji DD: Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica. J Clin Microbiol. 2009, 47: 1892-1895. 10.1128\/JCM.00105-09.","journal-title":"J Clin Microbiol"},{"key":"2560_CR23","doi-asserted-by":"crossref","first-page":"972","DOI":"10.4269\/ajtmh.2007.77.972","volume":"77","author":"DH Paris","year":"2007","unstructured":"Paris DH, Imwong M, Faiz AM, Hasan M, Yunus EB, Silamut K, Lee SJ, Day NP, Dondorp AM: Loop-mediated isothermal PCR (LAMP) for the diagnosis of falciparum malaria. Am J Trop Med Hyg. 2007, 77: 972-976.","journal-title":"Am J Trop Med Hyg"},{"issue":"Suppl 6","key":"2560_CR24","doi-asserted-by":"crossref","first-page":"119","DOI":"10.4269\/ajtmh.2007.77.119","volume":"77","author":"C Wongsrichanalai","year":"2007","unstructured":"Wongsrichanalai C, Barcus MJ, Muth S, Sutamihardja A, Wernsdorfer WH: A review of malaria diagnostic tools: microscopy and rapid diagnostic test (RDT). Am J Trop Med Hyg. 2007, 77 (Suppl 6): 119-127.","journal-title":"Am J Trop Med Hyg"},{"key":"2560_CR25","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/00034983.1983.11811683","volume":"77","author":"JA Collier","year":"1983","unstructured":"Collier JA, Longmore JM: The reliability of the microscopic diagnosis of malaria in the filed and in the laboratory. Ann Trop Med Parasitol. 1983, 77: 113-117.","journal-title":"Ann Trop Med Parasitol"},{"key":"2560_CR26","first-page":"621","volume":"66","author":"D Payne","year":"1988","unstructured":"Payne D: Use and limitations of light microscopy for diagnosing malaria at the primary health care level. Bull World Health Organ. 1988, 66: 621-626.","journal-title":"Bull World Health Organ"},{"key":"2560_CR27","doi-asserted-by":"publisher","first-page":"2866","DOI":"10.1128\/JCM.00355-10","volume":"48","author":"SD Polley","year":"2010","unstructured":"Polley SD, Mori Y, Watson J, Perkins MD, Gonzalez IJ, Notomi T, Chiodini PL, Sutherland CJ: Mitochondrial DNA targets increase sensitivity of malaria detection using loop-mediated isothermal amplification. J Clin Microbiol. 2010, 48: 2866-2871. 10.1128\/JCM.00355-10.","journal-title":"J Clin Microbiol"},{"key":"2560_CR28","doi-asserted-by":"publisher","first-page":"56","DOI":"10.4269\/ajtmh.2010.09-0630","volume":"83","author":"B Poschl","year":"2010","unstructured":"Poschl B, Waneesorn J, Thekisoe O, Chutipongvivate S, Karanis P: Comparative diagnosis of malaria infections by microscopy nested PCR, and LAMP in northern Thailand. Am J Trop Med Hyg. 2010, 83: 56-60. 10.4269\/ajtmh.2010.09-0630.","journal-title":"Am J Trop Med Hyg"},{"key":"2560_CR29","doi-asserted-by":"publisher","first-page":"594","DOI":"10.4269\/ajtmh.2011.10-0676","volume":"85","author":"J Sirichaisinthop","year":"2011","unstructured":"Sirichaisinthop J, Buates S, Watanabe R, Han ET, Suktawonjaroenpon W, Krasaesub S, Takeo S, Tsuboi T, Sattabongkot J: Evaluation of loop mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting. Am J Trop Med Hyg. 2011, 85: 594-596. 10.4269\/ajtmh.2011.10-0676.","journal-title":"Am J Trop Med Hyg"},{"key":"2560_CR30","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.actatropica.2012.02.004","volume":"122","author":"R Abdul-Ghani","year":"2012","unstructured":"Abdul-Ghani R, Al-Mekhlafi AM, Karanis P: Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: Would it come to clinical reality as a point-of-care test?. Acta Trop. 2012, 122: 233-240. 10.1016\/j.actatropica.2012.02.004.","journal-title":"Acta Trop"},{"key":"2560_CR31","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1186\/1475-2875-10-323","volume":"10","author":"S Proux","year":"2011","unstructured":"Proux S, Suwanarusk R, Barends M, Zwang J, Price RN, Leimanis M, Kiricharoen L, Laochan N, Russell B, Nosten F, Snounou G: Considerations on the use of nucleic acid-based amplification for malaria parasite detection. Malar J. 2011, 10: 323-10.1186\/1475-2875-10-323.","journal-title":"Malar J"},{"key":"2560_CR32","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1520\/JFS13607J","volume":"39","author":"A Akane","year":"1994","unstructured":"Akane A, Matsubara K, Nakamura H, Takahashi S, Kimura K: Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains a major inhibitor of polymerase chain reaction (PCR) amplification. J Forensic Sci. 1994, 39: 362-372.","journal-title":"J Forensic Sci"},{"key":"2560_CR33","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/JCM.38.1.345-350.2000","volume":"38","author":"W Abu Al-Soud","year":"2000","unstructured":"Abu Al-Soud W, J\u00f6nsson LJ, R\u00e5dstr MP: Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR. J Clin Microbiol. 2000, 38: 345-350.","journal-title":"J Clin Microbiol"},{"key":"2560_CR34","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1128\/JCM.39.2.485-493.2001","volume":"39","author":"W Abu Al-Soud","year":"2001","unstructured":"Abu Al-Soud W, R\u00e5dstro MP: Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol. 2001, 39: 485-493. 10.1128\/JCM.39.2.485-493.2001.","journal-title":"J Clin Microbiol"},{"key":"2560_CR35","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1128\/CMR.00035-07","volume":"21","author":"CK Murray","year":"2008","unstructured":"Murray CK, Gasser RA, Magill AJ, Miller RS: Update on rapid diagnostic testing for malaria. Clin Microbiol Rev. 2008, 21: 97-110. 10.1128\/CMR.00035-07.","journal-title":"Clin Microbiol Rev"},{"issue":"suppl","key":"2560_CR36","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1038\/nrmicro1524","volume":"4","author":"D Bell","year":"2006","unstructured":"Bell D, Peeling RW: Evaluation of rapid diagnostic tests: malaria. Nature Rev Microbiol. 2006, 4 (suppl): 34-38.","journal-title":"Nature Rev Microbiol"},{"key":"2560_CR37","volume-title":"Methods of field trials of malaria rapid diagnostic tests","author":"World Health Organization","year":"2009","unstructured":"World Health Organization: Methods of field trials of malaria rapid diagnostic tests. 2009, Geneva: World Health Organization"},{"key":"2560_CR38","doi-asserted-by":"publisher","first-page":"e204","DOI":"10.1371\/journal.pmed.0020204","volume":"2","author":"AM Dondorp","year":"2005","unstructured":"Dondorp AM, Desakorn V, Pongtavornpinyo W, Sahassananda D, Silamut K, Chotivanich K, Newton PN, Pitisuttithum P, Smithyman AM, White NJ, Day NPJ: Estimation of the total parasite biomass in acute falciparum malaria from plasma PfHRP2. PLoS Med. 2005, 2: e204-10.1371\/journal.pmed.0020204.","journal-title":"PLoS Med"},{"key":"2560_CR39","doi-asserted-by":"publisher","first-page":"e1001297","DOI":"10.1371\/journal.pmed.1001297","volume":"9","author":"ICE Hendriksen","year":"2012","unstructured":"Hendriksen ICE, Mwanga-Amumpaire J, von Seidlein L, Mtove G, White LJ, Olaosebikan R, Lee SJ, Tshefu AK, Woodrow C, Amos B, Karema C, Saiwaew S, Maitland K, Gomes E, Pan-Ngum W, Gesase S, Silamut K, Reyburn H, Joseph S, Chotivanich K, Fanello CI, Day NPJ, White NJ, Dondorp AM: Diagnosing severe falciparum malaria in parasitaemic African children: a prospective evaluation of plasma PfHRP2 measurement. PLoS Med. 2012, 9: e1001297-10.1371\/journal.pmed.1001297.","journal-title":"PLoS Med"},{"issue":"suppl","key":"2560_CR40","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/nrmicro1525","volume":"4","author":"D Bell","year":"2006","unstructured":"Bell D, Wongsrichanalai C, Barnwell JW: Ensuring quality and access for malaria diagnosis: how can it be achieved?. Nature Rev Microbiol. 2006, 4 (suppl): 7-20.","journal-title":"Nature Rev Microbiol"},{"key":"2560_CR41","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1186\/1475-2875-10-286","volume":"10","author":"J Luchavez","year":"2011","unstructured":"Luchavez J, Baker J, Alcantara S, Alcantara S, Belizario V, McCarthy JS, Bell D: Laboratory demonstration of a prozone-like effect in HRP2-detecting malaria rapid diagnostic tests: implications for clinical management. Malar J. 2011, 10: 286-10.1186\/1475-2875-10-286.","journal-title":"Malar J"},{"key":"2560_CR42","doi-asserted-by":"publisher","first-page":"194","DOI":"10.4269\/ajtmh.2012.10-0665","volume":"86","author":"OA Koita","year":"2012","unstructured":"Koita OA, Doumbo OK, Ouattara A, Tall LK, Konare A, Diakite M, Diallo M, Sagara I, Masinde GL, Doumbo SN, Dolo A, Tounkara A, Traore I, Krogstad DJ: False-negative rapid diagnostic tests for malaria and deletion of the histidine-rich repeat region of the hrp2 gene. Am J Trop Med Hyg. 2012, 86: 194-198. 10.4269\/ajtmh.2012.10-0665.","journal-title":"Am J Trop Med Hyg"},{"key":"2560_CR43","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1186\/1475-2875-6-29","volume":"6","author":"S Oyakhirome","year":"2007","unstructured":"Oyakhirome S, Potschke M, Schwarz NG, Dornemann J, Laengin M, Salazar CO, Lell B, Kun JF, Kremsner PG, Grobusch MP: Artesunate-amodiaquine combination therapy for falciparum malaria in young Gabonese children. Malar J. 2007, 6: 29-10.1186\/1475-2875-6-29.","journal-title":"Malar J"},{"key":"2560_CR44","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1186\/1475-2875-10-210","volume":"10","author":"SE Kern","year":"2011","unstructured":"Kern SE, Tiono AB, Makanga M, Gbadoe AD, Premji Z, Gaye O, Sagara I, Ubben D, Cousin M, Oladiran F, Sander O, Ogutu B: Community screening and treatment of asymptomatic carriers of Plasmodium falciparum with artemether- lumefantrine to reduce malaria disease burden: a modeling and simulation analysis. Malar J. 2011, 10: 210-10.1186\/1475-2875-10-210.","journal-title":"Malar J"}],"container-title":["Malaria Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1475-2875-11-408.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T20:59:00Z","timestamp":1630529940000},"score":1,"resource":{"primary":{"URL":"https:\/\/malariajournal.biomedcentral.com\/articles\/10.1186\/1475-2875-11-408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["2560"],"URL":"https:\/\/doi.org\/10.1186\/1475-2875-11-408","relation":{},"ISSN":["1475-2875"],"issn-type":[{"value":"1475-2875","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"21 September 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 December 2012","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 December 2012","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"408"}}