{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:14:25Z","timestamp":1761488065655},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Malar J"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Diligent and correct laboratory diagnosis and up-front identification of risk factors for progression to severe disease are the basis for optimal management of malaria.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Febrile children presenting to the Medical Research Unit at the Albert Schweitzer Hospital (HAS) in Lambar\u00e9n\u00e9, Gabon, were assessed for malaria. Giemsa-stained thick films for qualitative and quantitative diagnosis and enumeration of malaria pigment, or haemozoin (Hz)-containing leukocytes (PCL) were performed, and full blood counts (FBC) were generated with a Cell Dyn 3000<jats:sup>\u00ae<\/jats:sup> instrument.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Compared to standard light microscopy of Giemsa-stained thick films, diagnosis by platelet count only, by malaria pigment-containing monocytes (PCM) only, or by pigment-containing granulocytes (PCN) only yielded sensitivities\/specificities of 92%\/93%; 96%\/96%; and 85%\/96%, respectively. The platelet count was significantly lower in children with malaria compared to those without (p &lt; 0.001), and values showed little overlap between groups. Compared to microscopy, scatter flow cytometry as applied in the Cell-Dyn 3000<jats:sup>\u00ae<\/jats:sup> instrument detected significantly more patients with PCL (p &lt; 0.01). Both PCM and PCN numbers were higher in severe versus non-severe malaria yet reached statistical significance only for PCN (p &lt; 0.0001; PCM: p = 0.14). Of note was the presence of another, so far ill-defined pigment-containing group of phagocytic cells, identified by laser-flow cytometry as lymphocyte-like gated events, and predominantly found in children with malaria-associated anaemia.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>In the age group examined in the Lambar\u00e9n\u00e9 area, platelets are an excellent adjuvant tool to diagnose malaria. Pigment-containing leukocytes (PCL) are more readily detected by automated scatter flow cytometry than by microscopy. Automated Hz detection by an instrument as used here is a reliable diagnostic tool and correlates with disease severity. However, clinical usefulness as a prognostic tool is limited due to an overlap of PCL numbers recorded in severe versus non-severe malaria. However, this is possibly because of the instrument detection algorithm was not geared towards this task, and data lost during processing; and thus adjusting the instrument's algorithm may allow to establish a meaningful cut-off value.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1475-2875-7-109","type":"journal-article","created":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T06:13:30Z","timestamp":1213337610000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Full blood count and haemozoin-containing leukocytes in children with malaria: diagnostic value and association with disease severity"],"prefix":"10.1186","volume":"7","author":[{"given":"Thomas","family":"H\u00e4nscheid","sequence":"first","affiliation":[]},{"given":"Matthias","family":"L\u00e4ngin","sequence":"additional","affiliation":[]},{"given":"Bertrand","family":"Lell","sequence":"additional","affiliation":[]},{"given":"Marc","family":"P\u00f6tschke","sequence":"additional","affiliation":[]},{"given":"Sunny","family":"Oyakhirome","sequence":"additional","affiliation":[]},{"given":"Peter G","family":"Kremsner","sequence":"additional","affiliation":[]},{"given":"Martin P","family":"Grobusch","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2008,6,12]]},"reference":[{"key":"584_CR1","first-page":"83","volume":"295","author":"MP Grobusch","year":"2005","unstructured":"Grobusch MP, Kremsner PG: Uncomplicated malaria. Curr Topics Microbiol Immunol. 2005, 295: 83-104.","journal-title":"Curr Topics Microbiol Immunol"},{"key":"584_CR2","first-page":"23","volume-title":"Blood cells. A practical guide","author":"B Bain","year":"1995","unstructured":"Bain B: Blood cells. A practical guide. 1995, Oxford: Blackwell Science, 23-24."},{"key":"584_CR3","first-page":"137","volume":"11","author":"CL R\u00fcmke","year":"1985","unstructured":"R\u00fcmke CL: The imprecision of the ratio of two percentages observed in differential white blood cell counts: a warning. Blood Cells. 1985, 11: 137-140.","journal-title":"Blood Cells"},{"key":"584_CR4","first-page":"492","volume":"89","author":"G Giribaldi","year":"2004","unstructured":"Giribaldi G, Ulliers D, Schwarzer E, Roberts I, Piacibello W, Arese P: Hemozoin- and 4-hydroxynonenal-mediated inhibition of erythropoiesis. Possible role in malarial dyserythropoiesis and anemia. Haematologica. 2004, 89: 492-493.","journal-title":"Haematologica"},{"key":"584_CR5","doi-asserted-by":"publisher","first-page":"2569","DOI":"10.1182\/blood-2006-05-018697","volume":"108","author":"C Casals-Pascual","year":"2006","unstructured":"Casals-Pascual C, Kai O, Cheung JO, Williams S, Lowe B, Nyanoti M, Williams TN, Maitland K, Molyneux M, Newton CR, Peshu N, Watt SM, Roberts DJ: Suppression of erythropoiesis in malarial anemia is associated with hemozoin in vitro and in vivo. Blood. 2006, 108: 2569-2577. 10.1182\/blood-2006-05-018697.","journal-title":"Blood"},{"key":"584_CR6","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1128\/IAI.01327-06","volume":"75","author":"GA Awandare","year":"2007","unstructured":"Awandare GA, Ouma Y, Ouma C, Were T, Otieno R, Keller CC, Davenport GC, Hittner JB, Vulule J, Ferrell R, Ong'echa JM, Perkins DJ: Role of monocyte-acquired hemozoin in suppression of macrophage migration inhibitory factor in children with severe malarial anemia. Infect Immun. 2007, 75: 201-210. 10.1128\/IAI.01327-06.","journal-title":"Infect Immun"},{"key":"584_CR7","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/0035-9203(95)90423-9","volume":"89","author":"WG Metzger","year":"1995","unstructured":"Metzger WG, Mordm\u00fcller BG, Kremsner PG: Malaria pigment in leucocytes. Trans R Soc Trop Med Hyg. 1995, 89: 637-638. 10.1016\/0035-9203(95)90423-9.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"584_CR8","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/S0035-9203(98)90952-X","volume":"92","author":"OK Amodu","year":"1998","unstructured":"Amodu OK, Adeyemo AA, Olumese PE, Gbadegesin RA: Intraleucocytic malaria pigment and clinical severity of malaria in children. Trans R Soc Trop Med Hyg. 1998, 92: 54-56. 10.1016\/S0035-9203(98)90952-X.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"584_CR9","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4269\/ajtmh.2003.69.253","volume":"69","author":"KE Lyke","year":"2003","unstructured":"Lyke KE, Diallo DA, Dicko A, Kone A, Coulibaly D, Guindo A, Cissoko Y, Sangare L, Coulibaly S, Dakouo B, Taylor TE, Doumbo OK, Plowe CV: Association of intraleukocytic Plasmodium falciparum malaria pigment with disease severity, clinical manifestations, and prognosis in severe malaria. Am J Trop Med Hyg. 2003, 69: 253-259.","journal-title":"Am J Trop Med Hyg"},{"key":"584_CR10","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/S1473-3099(07)70238-4","volume":"7","author":"T H\u00e4nscheid","year":"2007","unstructured":"H\u00e4nscheid T, Egan TJ, Grobusch MP: Haemozoin: from melatonin pigment to drug target, diagnostic tool, and immune modulator. Lancet Infect Dis. 2007, 7: 675-685. 10.1016\/S1473-3099(07)70238-4.","journal-title":"Lancet Infect Dis"},{"key":"584_CR11","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/S0169-4758(00)01742-7","volume":"16","author":"T H\u00e4nscheid","year":"2000","unstructured":"H\u00e4nscheid T, Valadas E, Grobusch MP: Automated malaria diagnosis using pigment detection. Parasitol Today. 2000, 16: 549-551. 10.1016\/S0169-4758(00)01742-7.","journal-title":"Parasitol Today"},{"key":"584_CR12","doi-asserted-by":"crossref","first-page":"290","DOI":"10.4269\/ajtmh.2001.64.290","volume":"64","author":"T H\u00e4nscheid","year":"2001","unstructured":"H\u00e4nscheid T, Melo-Cristino J, Pinto BG: Automated detection of malaria pigment in white blood cells for the diagnosis of malaria in Portugal. Am J Trop Med Hyg. 2001, 64: 290-292.","journal-title":"Am J Trop Med Hyg"},{"key":"584_CR13","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1002\/cyto.b.10039","volume":"55","author":"MP Grobusch","year":"2003","unstructured":"Grobusch MP, H\u00e4nscheid T, Kr\u00e4mer B, Neukammer J, May J, Seybold J, Kun JF, Suttorp N: Sensitivity of hemozoin detection by automated flow cytometry in non-and semi-immune malaria patients. Cytometry B Clin Cytom. 2003, 55: 46-51. 10.1002\/cyto.b.10039.","journal-title":"Cytometry B Clin Cytom"},{"key":"584_CR14","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1099\/jmm.0.45650-0","volume":"54","author":"MM Padial","year":"2005","unstructured":"Padial MM, Subirats M, Puente S, Lago M, Crespo S, Palacios G, Baquero M: Sensitivity of laser light depolarization analysis for detection of malaria in blood samples. J Med Microbiol. 2005, 54: 449-452. 10.1099\/jmm.0.45650-0.","journal-title":"J Med Microbiol"},{"key":"584_CR15","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1111\/j.1365-3156.2006.01634.x","volume":"11","author":"AJ De Langen","year":"2006","unstructured":"De Langen AJ, van Dillen J, de Witte P, Mucheto S, Nagelkerke N, Kager P: Automated detection of malaria pigment: feasibility for malaria diagnosing in an area with seasonal malaria in northern Namibia. Trop Med Int Health. 2006, 11: 809-816. 10.1111\/j.1365-3156.2006.01634.x.","journal-title":"Trop Med Int Health"},{"key":"584_CR16","first-page":"77","volume":"46","author":"E Wildling","year":"1995","unstructured":"Wildling E, Winkler S, Kremsner PG, Brandts C, Jenne L, Wernsdorfer WH: Malaria epidemiology in the province Moyen Ogoue, Gabon. Trop Med Parasitol. 1995, 46: 77-82.","journal-title":"Trop Med Parasitol"},{"key":"584_CR17","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1016\/S0035-9203(00)90219-0","volume":"94","author":"EH Sylla","year":"2000","unstructured":"Sylla EH, Kun JF, Kremsner PG: Mosquito distribution and entomological inoculation rates in three malaria-endemic areas in Gabon. Trans R Soc Trop Med Hyg. 2000, 94: 652-656. 10.1016\/S0035-9203(00)90219-0.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"584_CR18","doi-asserted-by":"publisher","first-page":"1595","DOI":"10.1086\/522160","volume":"196","author":"MP Grobusch","year":"2007","unstructured":"Grobusch MP, Lell B, Schwarz N, Gabor J, D\u00f6rnemann J, P\u00f6tschke M, Oyakhirome S, Kiessling GC, Necek M, L\u00e4ngin M, Klein Klouwenberg P, Kl\u00f6pfer A, Naumann B, Altun H, Goesch J, Decker ML, Salazar CLO, Supan C, Kombila DU, Borchert L, K\u00f6ster KB, von Glasenapp I, Issifou S, Kremsner PG: Intermittent preventive treatment in infants of malaria in Gabon: a randomized, double-blind, placebo-controlled trial. J Infect Dis. 2007, 196: 1595-1602. 10.1086\/522160.","journal-title":"J Infect Dis"},{"issue":"suppl 1","key":"584_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0035-9203(00)90300-6","volume":"94","author":"World Health Organization, Division of Control of Tropical Diseases","year":"2000","unstructured":"World Health Organization, Division of Control of Tropical Diseases: Severe falciparum malaria. Trans R Soc Trop Med Hyg. 2000, 94 (suppl 1): 1-90. 10.1016\/S0035-9203(00)90300-6.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"584_CR20","doi-asserted-by":"crossref","first-page":"599","DOI":"10.4269\/ajtmh.2002.66.599","volume":"66","author":"T Planche","year":"2002","unstructured":"Planche T, Krishna S, Kombila M, Engel K, Faucher JF, Ngou-Milama E, Kremsner PG: Comparison of methods fort he rapid laboratory assessment of children with malaria. Am J Trop Med Hyg. 2002, 66: 599-602.","journal-title":"Am J Trop Med Hyg"},{"key":"584_CR21","volume-title":"Sequoia-Turner Corporation (Mountain View, CA)","author":"US, Patent: 5017497 Originators","year":"1989","unstructured":"US, Patent: 5017497 Originators: Gerard de Grooth; Bernard (Oldenzaal, NL); Greve; Jan (Oldenzaal, NL); Terstappen; Leonardus W. M. M. (Enschede, NL), \"Particle discriminator and method. Sequoia-Turner Corporation (Mountain View, CA). 1989"},{"key":"584_CR22","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1002\/cyto.990080602","volume":"8","author":"BG De Grooth","year":"1987","unstructured":"De Grooth BG, Terstappen LW, Puppels GJ, Greve J: Light-scattering polarization measurements as a new parameter in flow cytometry. Cytometry. 1987, 8: 539-544. 10.1002\/cyto.990080602.","journal-title":"Cytometry"},{"key":"584_CR23","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1538\/expanim.48.107","volume":"48","author":"S Suzuki","year":"1999","unstructured":"Suzuki S, Eguchi N: Leukocyte differential analysis in multiple laboratory species by a laser multi-angle polarized light scattering separation method. Exp Anim. 1999, 48: 107-114. 10.1538\/expanim.48.107.","journal-title":"Exp Anim"},{"key":"584_CR24","unstructured":"[http:\/\/rsb.info.nih.gov\/ij\/]"},{"key":"584_CR25","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1002\/1097-0320(20011001)45:2<133::AID-CYTO1155>3.0.CO;2-K","volume":"45","author":"B Kr\u00e4mer","year":"2001","unstructured":"Kr\u00e4mer B, Grobusch MP, Suttorp N, Neukammer J, Rinneberg H: Relative frequency of malaria pigment-carrying monocytes of nonimmune and semi-immune patients from flow cytometric depolarized side scatter. Cytometry. 2001, 45: 133-140. 10.1002\/1097-0320(20011001)45:2<133::AID-CYTO1155>3.0.CO;2-K.","journal-title":"Cytometry"},{"key":"584_CR26","first-page":"147","volume-title":"Blood cells. A practical guide","author":"B Bain","year":"1995","unstructured":"Bain B: Blood cells. A practical guide. 1995, Oxford: Blackwell Science, 147-159."},{"key":"584_CR27","doi-asserted-by":"publisher","first-page":"1741","DOI":"10.1111\/j.1365-3156.2006.01764.x","volume":"11","author":"L Quint\u00f3","year":"2006","unstructured":"Quint\u00f3 L, Aponte JJ, Sacarlal J, Espasa M, Aide P, Mandomando I, Guinovart C, Macete E, Navia MM, Thompson R, Men\u00e9ndez C, Alonso PL: Haematological and biochemical indices in young African children: in search of reference intervals. Trop Med Int Health. 2006, 11: 1741-1748. 10.1111\/j.1365-3156.2006.01764.x.","journal-title":"Trop Med Int Health"},{"key":"584_CR28","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1046\/j.1365-2257.2002.00466.x","volume":"24","author":"CS Scott","year":"2002","unstructured":"Scott CS, Van Zyl D, Ho E, Ruivo L, Mendelow B, Coetzer TL: Thrombocytopenia in patients with malaria: automated analysis of optical platelet counts and platelet clumps with the Cell Dyn CD4000 analyser. Clin Lab Haematol. 2002, 24: 295-302. 10.1046\/j.1365-2257.2002.00466.x.","journal-title":"Clin Lab Haematol"},{"key":"584_CR29","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1128\/jcm.17.5.717-721.1983","volume":"17","author":"GA Jamjoom","year":"1983","unstructured":"Jamjoom GA: Dark-field microscopy for detection of malaria in unstained blood films. J Clin Microbiol. 1983, 17: 717-721.","journal-title":"J Clin Microbiol"},{"key":"584_CR30","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.actatropica.2004.02.003","volume":"90","author":"C Romagosa","year":"2004","unstructured":"Romagosa C, Menendez C, Ismail MR, Quint\u00f3 L, Ferrer B, Alonso PL, Ordi J: Polarisation microscopy increases the sensitivity of hemozoin and Plasmodium detection in the histological assessment of placental malaria. Acta Trop. 2004, 90: 277-284. 10.1016\/j.actatropica.2004.02.003.","journal-title":"Acta Trop"},{"key":"584_CR31","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/0035-9203(95)90496-4","volume":"89","author":"NH Phu","year":"1995","unstructured":"Phu NH, Day N, Diep PT, Ferguson DJP, White NJ: Intraleukocytic malaria pigment and clinical severity of malaria in children. Trans R Soc Trop Med Hyg. 1995, 89: 200-204. 10.1016\/0035-9203(95)90496-4.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"584_CR32","doi-asserted-by":"crossref","first-page":"724","DOI":"10.4269\/ajtmh.2006.74.724","volume":"74","author":"G Mujuzi","year":"2006","unstructured":"Mujuzi G, Magambo B, Okech B, Egwang TG: Pigmented monocytes are negative correlates of protection against severe and complicated malaria in Ugandan children. Am J Trop Med Hyg. 2006, 74: 724-729.","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"584_CR33","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1046\/j.1365-2141.1999.01199.x","volume":"106","author":"BV Mendelow","year":"1999","unstructured":"Mendelow BV, Lyons C, Nhlangothi P, Tana M, Munster M, Wypkema E, Liebowitz L, Marshall L, Scott S, Coetzer TL: Automated malaria detection by depolarization of laser light. Br J Haematol. 1999, 106 (3): 499-503. 10.1046\/j.1365-2141.1999.01199.x.","journal-title":"Br J Haematol"},{"key":"584_CR34","doi-asserted-by":"publisher","first-page":"4729","DOI":"10.1128\/JCM.40.12.4729-4731.2002","volume":"40","author":"PC Wever","year":"2002","unstructured":"Wever PC, Henskens YM, Kager PA, Dankert J, van Gool T: Detection of imported malaria with the Cell-Dyn 4000 hematology analyzer. J Clin Microbiol. 2002, 40: 4729-4731. 10.1128\/JCM.40.12.4729-4731.2002.","journal-title":"J Clin Microbiol"},{"key":"584_CR35","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1046\/j.1365-2257.2003.00496.x","volume":"25","author":"Cs Scott","year":"2003","unstructured":"Scott Cs, van Zyl D, Ho E, Meyersfeld D, Ruivo L, Mendelow BV, Coetzer TL: Automated detection of malaria-associated intraleukocytic haemozoin By Cell-Dyn CD 4000 depolarization analysis. Clin Lab Haematol. 2003, 25: 77-86. 10.1046\/j.1365-2257.2003.00496.x.","journal-title":"Clin Lab Haematol"},{"key":"584_CR36","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1016\/j.trstmh.2004.07.009","volume":"99","author":"JA Dromigny","year":"2005","unstructured":"Dromigny JA, Jambou R, Scott CS, Perrier-Gros-Claude JD: Performance evaluation of automated depolarization analysis for detecting clinically unsuspected malaria in endemic countries. Trans R Soc Trop Med Hyg. 2005, 99: 430-439. 10.1016\/j.trstmh.2004.07.009.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"584_CR37","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.parint.2005.10.003","volume":"55","author":"NT Huy","year":"2006","unstructured":"Huy NT, Trang DT, Kariu T, Sasai M, Saida K, Harada S, Kamei K: Leukocyte activation by malarial pigment. Parasitol Int. 2006, 55: 75-81. 10.1016\/j.parint.2005.10.003.","journal-title":"Parasitol Int"},{"key":"584_CR38","doi-asserted-by":"crossref","first-page":"4694","DOI":"10.1182\/blood.V88.12.4694.bloodjournal88124694","volume":"88","author":"NP Day","year":"1996","unstructured":"Day NP, Pham TD, Phan TL, Dinh XS, Pham PL, Ly VC, Tran TH, Nguyen TH, Bethell DB, Nguyan HP, Tran TH, White NJ: Clearance kinetics of parasites and pigment-containing leukocytes in severe malaria. Blood. 1996, 88: 4694-4700.","journal-title":"Blood"},{"key":"584_CR39","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/0035-9203(95)90496-4","volume":"89","author":"PH Nguyen","year":"1995","unstructured":"Nguyen PH, Day N, Pram TD, Ferguson DJ, White NJ: Intraleucocytic malaria pigment and prognosis in severe malaria. Trans R Soc Trop Med Hyg. 1995, 89: 200-204. 10.1016\/0035-9203(95)90496-4.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"584_CR40","doi-asserted-by":"publisher","first-page":"4868","DOI":"10.1128\/IAI.72.8.4868-4873.2004","volume":"72","author":"CC Keller","year":"2004","unstructured":"Keller CC, Kremsner PG, Hittner JB, Misukonis MA, Weinberg JB, Perkins DJ: Elevated nitric oxide production in children with malarial anaemia: haemozoin-induced nitric oxide synthase type 2 transcripts and nitric oxide in blood mononuclear cells. Infect Immun. 2004, 72: 4868-4873. 10.1128\/IAI.72.8.4868-4873.2004.","journal-title":"Infect Immun"}],"container-title":["Malaria Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1475-2875-7-109.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T01:43:24Z","timestamp":1630460604000},"score":1,"resource":{"primary":{"URL":"https:\/\/malariajournal.biomedcentral.com\/articles\/10.1186\/1475-2875-7-109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6,12]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["584"],"URL":"https:\/\/doi.org\/10.1186\/1475-2875-7-109","relation":{},"ISSN":["1475-2875"],"issn-type":[{"value":"1475-2875","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6,12]]},"assertion":[{"value":"21 December 2007","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2008","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2008","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"109"}}