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The aim of the present study was to determine the protein expression changes induced by condensed tannin intake on the fraction of mouse whole salivary proteins that are unable to form insoluble tannin-protein complexes. Two-dimensional polyacrylamide gel electrophoresis protein separation was used, followed by protein identification by mass spectrometry.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Fifty-seven protein spots were excised from control group gels, and 21 different proteins were identified. With tannin consumption, the expression levels of one \u03b1-amylase isoform and one unidentified protein increased, whereas acidic mammalian chitinase and Muc10 decreased. Additionally, two basic spots that stained pink with Coomassie Brilliant Blue R-250 were newly observed, suggesting that some induced PRPs may remain uncomplexed or form soluble complexes with tannins.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This proteomic analysis provides evidence that other salivary proteins, in addition to tannin-precipitating proteins, are affected by tannin ingestion. Changes in the expression levels of the acidic mammalian chitinase precursor and in one of the 14 salivary \u03b1-amylase isoforms underscores the need to further investigate their role in tannin ingestion.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1477-5956-8-65","type":"journal-article","created":{"date-parts":[[2011,1,19]],"date-time":"2011-01-19T11:07:51Z","timestamp":1295435271000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Changes in mouse whole saliva soluble proteome induced by tannin-enriched diet"],"prefix":"10.1186","volume":"8","author":[{"given":"Elsa","family":"Lamy","sequence":"first","affiliation":[]},{"given":"Gon\u00e7alo","family":"Gra\u00e7a","sequence":"additional","affiliation":[]},{"given":"Gon\u00e7alo","family":"da Costa","sequence":"additional","affiliation":[]},{"given":"Catarina","family":"Franco","sequence":"additional","affiliation":[]},{"given":"Fernando Capela","family":"e Silva","sequence":"additional","affiliation":[]},{"given":"Elvira Sales","family":"Baptista","sequence":"additional","affiliation":[]},{"given":"Ana Varela","family":"Coelho","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,12,15]]},"reference":[{"key":"217_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/icb.1981.1","volume":"59","author":"JA Young","year":"1981","unstructured":"Young JA, Schneyer CA: Composition of saliva in mammalian. 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