{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T01:01:42Z","timestamp":1769043702777,"version":"3.49.0"},"reference-count":55,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2012,1,1]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p> It was recently reported that the mono-iodoacetate (MIA) experimental model of osteoarthritis (OA) courses with changes of neurons innervating the affected joints that are commonly interpreted as a neuronal response to axonal injury. To better characterize these changes, we evaluated the expression of two markers of neuronal damage, ATF-3 and NPY, and the growth associated protein GAP-43, in primary afferent neurons of OA animals injected with three different doses of MIA (0.3, 1 or 2 mg). Measurements were performed at days 3, 7, 14, 21 and 31 post-MIA injection. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> OA animals showed the characteristic histopathological changes of the joints and the accompanying nociceptive behaviour, evaluated by the Knee-Bed and CatWalk tests. An increase of ATF-3 expression was detected in the DRG of OA animals as early as 3 days after the injection of 1 or 2 mg of MIA and 7 days after the injection of 0.3 mg. NPY expression was increased in animals injected with 1 or 2 mg of MIA, at day 3 or in all time-points, respectively. From day 7 onwards there was a massive increase of GAP-43 expression in ATF-3 cells. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p> The expression of the neuronal injury markers ATF-3 and NPY as well as an up-regulation of GAP-43 expression, indicative of peripheral fibre regeneration, suggests that axonal injury and a regeneration response may be happening in this model of OA. This opens new perspectives in the unravelling of the physiopathology of the human disease. <\/jats:p><\/jats:sec>","DOI":"10.1186\/1744-8069-8-50","type":"journal-article","created":{"date-parts":[[2012,7,8]],"date-time":"2012-07-08T18:14:15Z","timestamp":1341771255000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":59,"title":["Dose-Dependent Expression of Neuronal Injury Markers during Experimental Osteoarthritis Induced by Monoiodoacetate in the Rat"],"prefix":"10.1177","volume":"8","author":[{"given":"Joana","family":"Ferreira-Gomes","sequence":"first","affiliation":[{"name":"Department of Experimental Biology, Faculty of Medicine of Porto and IBMC - Institute for Molecular and Cell Biology, University of Porto, Alameda Prof. Hernani Monteiro, Porto 4200-319, Portugal"}]},{"given":"Sara","family":"Ad\u00e3es","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology, Faculty of 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