{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:48:55Z","timestamp":1762375735819},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Wall lizards of genus <jats:italic>Podarcis<\/jats:italic> are abundant and conspicuous reptiles inhabiting Europe and North Africa. In recent years, they have become a popular lizard model for phylogeographical and evolutionary ecology studies. However a lack of suitable nuclear markers currently presents a limitation on analyses of molecular evolution within this genus. We address this limitation by developing twenty-one new primer pairs for polymerase chain reaction (PCR) amplification and sequencing of anonymous sequence markers in <jats:italic>Podarcis vaucheri<\/jats:italic> and performed an assay of their cross-amplification and polymorphism levels in two closely- (<jats:italic>P. bocagei<\/jats:italic> and <jats:italic>P. liolepis<\/jats:italic>) and two distantly-related (<jats:italic>P. muralis<\/jats:italic> and <jats:italic>P. tiliguerta<\/jats:italic>) congeners.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Findings<\/jats:title>\n            <jats:p>Cross-amplification and sequencing was straightforward among members of the Iberian and North-African group within genus <jats:italic>Podarcis<\/jats:italic> (which includes <jats:italic>P. vaucheri<\/jats:italic>), and somewhat less successful in species belonging to other groups (one and four loci out of 21 failed to amplify in <jats:italic>P. muralis<\/jats:italic> and <jats:italic>P. tiliguerta<\/jats:italic>, respectively, and overall success rates were lower). Nucleotide diversity for the five species examined ranged from 0.35% to 3.5%, with an average of 1.5% across all loci. Insertion and deletion polymorphisms were found in all but three loci.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Given the high cross-amplification rates, these markers constitute a valuable addition to set of genomic resources available for <jats:italic>Podarcis<\/jats:italic>, especially in studies dealing with phylogenetics, species delimitation, population genetics and phylogeography.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-0500-6-299","type":"journal-article","created":{"date-parts":[[2013,7,27]],"date-time":"2013-07-27T16:14:02Z","timestamp":1374941642000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Twenty-one new sequence markers for population genetics, species delimitation and phylogenetics in wall lizards (Podarcis spp.)"],"prefix":"10.1186","volume":"6","author":[{"given":"Carolina","family":"Pereira","sequence":"first","affiliation":[]},{"given":"Alvarina","family":"Couto","sequence":"additional","affiliation":[]},{"given":"Carla","family":"Lu\u00eds","sequence":"additional","affiliation":[]},{"given":"Diogo","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Sofia","family":"Mour\u00e3o","sequence":"additional","affiliation":[]},{"given":"Catarina","family":"Pinho","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2013,7,27]]},"reference":[{"key":"2312_CR1","doi-asserted-by":"publisher","first-page":"3250","DOI":"10.1111\/j.1365-294X.2010.04722.x","volume":"19","author":"A Camargo","year":"2010","unstructured":"Camargo A, Sinervo B, Sites Jr JW: Lizards as model organisms for linking phylogeographic and speciation studies. 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