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However, manually counting the eggs is time consuming, tedious, and error prone.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>In this paper we present a newly developed software for high precision automatic egg counting. The software written in the Java programming language proposes a user-friendly interface and a complete online manual. It allows the inspection of results by the operator and includes proper tools for manual corrections. The user can in fact correct any details on the acquired results by a mouse click. Time saving is significant and errors due to loss of concentration are avoided.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The software was tested over 16 randomly chosen images from 2 different experiments. The results show that the proposed automatic method produces results that are close to the ground truth.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The proposed approaches demonstrated a very high level of robustness. The adoption of the proposed software package will save many hours of labor to the bench scientist. The software needs no particular configuration and is freely available for download on: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/w3.ualg.pt\/~hshah\/eggcounter\/\" ext-link-type=\"uri\">http:\/\/w3.ualg.pt\/\u223chshah\/eggcounter\/<\/jats:ext-link>.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-5-122","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T14:15:03Z","timestamp":1340806503000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["A user-friendly software to easily count Anopheles egg batches"],"prefix":"10.1186","volume":"5","author":[{"given":"Ali","family":"Mollahosseini","sequence":"first","affiliation":[]},{"given":"Marie","family":"Rossignol","sequence":"additional","affiliation":[]},{"given":"C\u00e9dric","family":"Pennetier","sequence":"additional","affiliation":[]},{"given":"Anna","family":"Cohuet","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio dos","family":"Anjos","sequence":"additional","affiliation":[]},{"given":"Fabrice","family":"Chandre","sequence":"additional","affiliation":[]},{"given":"Hamid Reza","family":"Shahbazkia","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2012,6,19]]},"reference":[{"issue":"8","key":"649_CR1","doi-asserted-by":"publisher","first-page":"e1000303","DOI":"10.1371\/journal.pmed.1000303","volume":"7","author":"HM Ferguson","year":"2010","unstructured":"Ferguson HM, Dornhaus A, Beeche A, Borgemeister C, Gottlieb M, Mulla MS, Gimnig JE, Fish D, Killeen GF: Ecology: A prerequisite for malaria elimination and eradication. 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