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In this study, the feeding patterns of molestus and pipiens forms were investigated in Comporta (Portugal), where high levels of inter-form admixture have been recorded.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Indoor and outdoor mosquito collections were performed in the summer of 2010. Collected<jats:italic>Cx. pipiens s.l.<\/jats:italic>females were molecularly identified to species and form by PCR and genotyped for six microsatellites. The source of the blood meal in post-fed females was determined by ELISA and mitochondrial DNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The distribution of the forms differed according to the collection method. The molestus form was present only in indoor collections, whereas pipiens and admixed individuals were sampled both indoors and outdoors. In both forms, over 90% of blood meals were made on avian hosts. These included blood meals taken from Passeriformes (<jats:italic>Passer domesticus<\/jats:italic>and<jats:italic>Turdus merula<\/jats:italic>) by females caught resting inside domestic shelters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Genetic structure and blood meal analyses suggest the presence of a bird biting molestus population in the study area. Both forms were found to rest indoors, mainly in avian shelters, but at least a proportion of females of the pipiens form may bite outdoors in sylvan habitats and then search for anthropogenic resting sites to complete their gonotrophic cycle. This behaviour may potentiate the accidental transmission of arboviruses to humans in the region.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1756-3305-6-93","type":"journal-article","created":{"date-parts":[[2013,4,11]],"date-time":"2013-04-11T16:14:51Z","timestamp":1365696891000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Feeding patterns of molestus and pipiens forms of Culex pipiens (Diptera: Culicidae) in a region of high hybridization"],"prefix":"10.1186","volume":"6","author":[{"given":"Bruno","family":"Gomes","sequence":"first","affiliation":[]},{"given":"Carla A","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 L","family":"Vicente","sequence":"additional","affiliation":[]},{"given":"Leonor","family":"Pinho","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Calder\u00f3n","sequence":"additional","affiliation":[]},{"given":"Eliane","family":"Arez","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio PG","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Martin J","family":"Donnelly","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Pinto","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2013,4,11]]},"reference":[{"key":"894_CR1","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1111\/j.0269-283X.2005.00547.x","volume":"19","author":"JM Medlock","year":"2005","unstructured":"Medlock JM, Snow KR, Leach S: Potential transmission of West Nile virus in the British Isles: an ecological review of candidate mosquito bridge vectors. 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