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To evaluate the ability of such material as a substrate for bacterial colonization, this work assessed the capability of different bacteria to colonize a biomimetic rough superhydrophobic (SH) PLLA surface and also a smooth hydrophobic (H) one. The interaction between these surfaces and bacteria with different morphologies and cell walls was studied using one strain of <jats:italic>Staphylococcus aureus<\/jats:italic> and one of <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. Results showed that both bacterial strains colonized the surfaces tested, although significantly higher numbers of <jats:italic>S. aureus<\/jats:italic> cells were found on SH surfaces comparing to H ones. Moreover, scanning electron microscopy images showed an extracellular matrix produced by <jats:italic>P. aeruginosa<\/jats:italic> on SH PLLA surfaces, indicating that this bacterium is able to form a biofilm on such substratum. Bacterial removal through lotus leaf effect was also tested, being more efficient on H coupons than on SH PLLA ones. Overall, the results showed that SH PLLA surfaces can be used as a substrate for bacterial colonization and, thus, have an exceptional potential for biotechnology applications.<\/jats:p>","DOI":"10.1186\/2191-0855-1-34","type":"journal-article","created":{"date-parts":[[2011,10,22]],"date-time":"2011-10-22T18:14:36Z","timestamp":1319307276000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Superhydrophobic poly(L-lactic acid) surface as potential bacterial colonization substrate"],"prefix":"10.1186","volume":"1","author":[{"given":"Cl\u00e1udia","family":"Sousa","sequence":"first","affiliation":[]},{"given":"Diana","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Ros\u00e1rio","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Wenlong","family":"Song","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o F","family":"Mano","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Azeredo","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,10,22]]},"reference":[{"key":"40_CR1","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1080\/09593330309385575","volume":"24","author":"IS Ahn","year":"2003","unstructured":"Ahn IS, Lee CH: Kinetic studies of attachment and detachment of microbial cells from soil. 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