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Among the TLR family, TLR5 is the only that senses and recognizes flagellin, the major protein of bacterial flagella. TLR5 has been reported to be under overall purifying selection in mammals, with a small proportion of codons under positive selection. However, the variation of substitution rates among major mammalian groups has been neglected. Here, we studied the evolution of TLR5 in mammals, comparing the substitution rates among groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study we analysed the TLR5 substitution rates in Euungulata, Carnivora, Chiroptera, Primata, Rodentia and Lagomorpha, groups. For that, Tajima\u2019s relative rate test, Bayesian inference of evolutionary rates and genetic distances were estimated with CODEML\u2019s branch model and RELAX. The combined results showed that in the Lagomorpha, Rodentia, Carnivora and Chiroptera lineages TLR5 is evolving at a higher substitution rate. The RELAX analysis further suggested a significant relaxation of selective pressures for the Lagomorpha (K\u2009=\u20090.22,<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01), Rodentia (K\u2009=\u20090.58,<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01) and Chiroptera (K\u2009=\u20090.65,<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01) lineages and for the Carnivora ancestral branches (K\u2009=\u20090.13,<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results show that the TLR5 substitution rate is not uniform among mammals. In fact, among the different mammal groups studied, the Lagomorpha, Rodentia, Carnivora and Chiroptera are evolving faster. This evolutionary pattern could be explained by 1) the acquisition of new functions of TLR5 in the groups with higher substitution rate, i.e. TLR5 neofunctionalization, 2) by the beginning of a TLR5 pseudogenization in these groups due to some redundancy between the TLRs genes, or 3) an arms race between TLR5 and species-specific parasites.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12862-019-1547-4","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T18:03:14Z","timestamp":1575309794000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Analysis of substitution rates showed that TLR5 is evolving at different rates among mammalian groups"],"prefix":"10.1186","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9047-954X","authenticated-orcid":false,"given":"Ana","family":"Pinheiro","sequence":"first","affiliation":[]},{"given":"Ana","family":"\u00c1gueda-Pinto","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9","family":"Melo-Ferreira","sequence":"additional","affiliation":[]},{"given":"Fabiana","family":"Neves","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Abrantes","sequence":"additional","affiliation":[]},{"given":"Pedro J.","family":"Esteves","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,12,2]]},"reference":[{"key":"1547_CR1","doi-asserted-by":"publisher","first-page":"2379","DOI":"10.3389\/fimmu.2018.02379","volume":"9","author":"GP Amarante-Mendes","year":"2018","unstructured":"Amarante-Mendes GP, Adjemian S, Branco LM, Zanetti LC, Weinlich R, Bortoluci KR. 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