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In Mozambique over one hundred species of potential arbovirus mosquito vectors have been identified, although their precise role in maintaining such viruses in circulation in the country remains to be elucidated. The aim of this study was to screen for the presence of flaviviruses, alphaviruses and bunyaviruses in mosquitoes from different regions of Mozambique.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Our survey analyzed 14,519 mosquitoes, and the results obtained revealed genetically distinct insect-specific flaviviruses, detected in multiple species of mosquitoes from different genera. In addition, smaller flavivirus-like <jats:italic>NS5<\/jats:italic> sequences, frequently detected in <jats:italic>Mansonia<\/jats:italic> seemed to correspond to defective viral sequences, present as viral DNA forms. Furthermore, three lineages of putative members of the <jats:italic>Phenuiviridae<\/jats:italic> family were also detected, two of which apparently corresponding to novel viral genetic lineages.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study reports for the first-time novel insect-specific flaviviruses and novel phenuiviruses, as well as frequent flavivirus-like viral DNA forms in several widely known vector species. This unique work represents recent investigation of virus screening conducted in mosquitoes from Mozambique and an important contribution to inform the establishment of a vector control program for arbovirus in the country and in the region.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-020-01905-5","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T07:51:47Z","timestamp":1595922707000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A survey of RNA viruses in mosquitoes from Mozambique reveals novel genetic lineages of flaviviruses and phenuiviruses, as well as frequent flavivirus-like viral DNA forms in Mansonia"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3652-1329","authenticated-orcid":false,"given":"Ana Paula","family":"Ab\u00edlio","sequence":"first","affiliation":[]},{"given":"Manuel","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Ayubo","family":"Kampango","sequence":"additional","affiliation":[]},{"given":"In\u00e1cio","family":"Narciso","sequence":"additional","affiliation":[]},{"given":"Eduardo Samo","family":"Gudo","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds Carlos Bernardo","family":"das Neves","sequence":"additional","affiliation":[]},{"given":"Mohsin","family":"Sidat","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Manuel","family":"Fafetine","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio Paulo Gouveia","family":"de Almeida","sequence":"additional","affiliation":[]},{"given":"Ricardo","family":"Parreira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,7,28]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Campbell-Lendrum D, Manga L, Bagayoko M, Sommerfeld J. 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