{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T23:05:46Z","timestamp":1778540746492,"version":"3.51.4"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:00:00Z","timestamp":1682985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:00:00Z","timestamp":1682985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Complement Med Ther"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Plants of the <jats:italic>Myrcia<\/jats:italic> genus have been widely used in folk medicine to treat various diseases, including cancer. <jats:italic>Myrcia splendens<\/jats:italic> species has a diverse chemical constitution, but the biological activities of its essential oil have not been well investigated. In this study to out the chemistry characterization of essential oil (EO) from the leaves of the species <jats:italic>M. splendens<\/jats:italic> from Brazil and evaluate cytotoxic effect in A549 lung cancer cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p><jats:italic>M. splendens<\/jats:italic> EO was obtained by hydrodistillation and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). EO was isolated and evaluated for cellular viability in tumor cell lines by MTT assay. The evaluation of the formation of clones and the migratory capacity of the A549 cells treated with EO was done by the clonogenic assay and the wound healing assay. Morphological changes were observed in A549 cells by fluorescence using Phalloidin\/FITC and DAPI.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>22 compounds were identified in the chemical analysis of EO, corresponding to 88% of the sample. Major compounds were the sesquiterpenic hydrocarbons bicyclogermacrene (15.4%), germacrene D (8.9%) and E-caryophyllene (10.1%). The biological analysis of the EO showed high cytotoxic activity with an IC<jats:sub>50<\/jats:sub> below 20\u00a0\u00b5g\/ml in the THP-1, A549 and B16-F10 tumor cells. The treatment with EO reduced colony formation and inhibited the migratory capacity of A549 cells. Furthermore, apoptotic morphological changes in the nucleus and cytoplasm of A549 cells was observed after of treatment with EO.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The findings of this study suggest that the <jats:italic>M. splendens<\/jats:italic> EO has cytotoxic compounds for the A549 lung cancer cells. Treatment with the EO decreased the colony formation and reduced the ability of lung cancer cells to migrate. Future studies may be used to isolate compounds from the EO for the study of lung cancer.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12906-023-03969-y","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T09:02:34Z","timestamp":1683018154000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cytotoxic activity of essential oil from Leaves of Myrcia splendens against A549 Lung Cancer cells"],"prefix":"10.1186","volume":"23","author":[{"given":"Monalisa Martins","family":"Montalv\u00e3o","sequence":"first","affiliation":[]},{"given":"Franciel Batista","family":"Felix","sequence":"additional","affiliation":[]},{"given":"Edmilson William","family":"Propheta dos Santos","sequence":"additional","affiliation":[]},{"given":"Jileno Ferreira","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Waldecy","family":"de Lucca J\u00fanior","sequence":"additional","affiliation":[]},{"given":"Atenilton Santos","family":"Farias","sequence":"additional","affiliation":[]},{"given":"Adauto","family":"de Souza Ribeiro","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Cavaleiro","sequence":"additional","affiliation":[]},{"given":"Sam\u00edsia Maria Fernandes","family":"Machado","sequence":"additional","affiliation":[]},{"given":"Ricardo","family":"Scher","sequence":"additional","affiliation":[]},{"given":"Cristiane Bani","family":"Corr\u00eaa","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,5,2]]},"reference":[{"key":"3969_CR1","doi-asserted-by":"crossref","unstructured":"Regnard C, Kindlen M. 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S. do Socorro, Sergipe, Brazil. The use of the leaves of species <i>Myrcia splendens<\/i> has been authorized by the Agency for Research in Federal Conservation Units (SISBIO) of the Chico Mendes Institute for Biodiversity Conservation (ICMBio) (collection authorization No. 68163\/2019 \u2013 SISBIO - ICMBio), and identified by Dr. Adauto de Souza Ribeiro, Federal University of Sergipe. A voucher specimen (ASE 33399) is deposited at the Herbarium of the Department of Biology, Federal University of Sergipe, Brazil. The experimental research and feld studies on plants, including the collection of plant material, comply with relevant institutional, national, and international guidelines and legislation.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors report no conflicts of interest of this work.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"139"}}