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Several strategies may be used for PD control, including dental hygiene home care procedures, like toothbrushing, special diet and chew toys that reduce dental plaque accumulation, or professional periodontal treatments. Aiming at PD control, a biogel composed by nisin and guar-gum was previously developed. This work aimed to establish an <jats:italic>in vitro<\/jats:italic> model mimicking the PD-associated biofilms and to evaluate the nisin-biogel inhibitory activity against this polymicrobial biofilm by determining its Minimum Biofilm Inhibitory (MBIC) and Eradication Concentrations (MBEC). Bacterial species tested included <jats:italic>Neisseria zoodegmatis<\/jats:italic> CCUG 52598T, <jats:italic>Corynebacterium canis<\/jats:italic> CCUG 58627T, <jats:italic>Porphyromonas cangingivalis<\/jats:italic> DSMZ VPB 4874, <jats:italic>Peptostreptococcus canis<\/jats:italic> CCUG 57081 and an <jats:italic>Enterococcus faecalis<\/jats:italic> isolate belonging to a collection of oral bacteria obtained from dogs with PD. Before establishing the biofilm, coaggregation between species was determined by optical density measurement after 2 and 24 hours. Nisin-biogel MBIC and MBEC values regarding the polymicrobial biofilm were determined using a modified version of the Calgary biofilm pin lid device, after confirming the presence of the five bacterial species by Fluorescent <jats:italic>In Situ<\/jats:italic> Hybridization.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Only 40% of the bacterial dual suspensions were able to coaggregate at 2 hours, but all species tested exhibited a coaggregation percentage higher than 30% at 24 hours. It was possible to establish a 48\u00a0h polymicrobial biofilm model composed by the five bacterial species selected. This model was used to determine nisin-biogel MBIC (26.39\u2009\u00b1\u20095.89\u00a0\u00b5g\/mL) and MBEC (62.5\u2009\u00b1\u200927.73\u00a0\u00b5g\/mL) values.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our results showed that the nisin-biogel can inhibit and eradicate PD multispecies biofilms. As this <jats:italic>in vitro<\/jats:italic> model mimics an <jats:italic>in vivo<\/jats:italic> periodontal polymicrobial biofilm, our results reinforce the potential of the application of nisin-biogel for canine PD control.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-020-02646-3","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T10:03:02Z","timestamp":1606903382000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A polymicrobial biofilm model for testing the antimicrobial potential of a nisin-biogel for canine periodontal disease control"],"prefix":"10.1186","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5641-4400","authenticated-orcid":false,"given":"Eva","family":"Cunha","sequence":"first","affiliation":[]},{"given":"Sandra","family":"Rebelo","sequence":"additional","affiliation":[]},{"given":"Carla","family":"Carneiro","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds","family":"Tavares","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds Miguel","family":"Carreira","sequence":"additional","affiliation":[]},{"given":"Manuela","family":"Oliveira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,12,2]]},"reference":[{"issue":"1","key":"2646_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12917-018-1692-9","volume":"14","author":"E Cunha","year":"2018","unstructured":"Cunha E, Trov\u00e3o T, Pinheiro A, Nunes T, Santos R, Moreira Da Silva J, S\u00e3o Braz B, Tavares L, Veiga AS, Oliveira M. 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