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However, the majority of studies documents the use of<jats:italic>C. vulgaris<\/jats:italic>as a dietary supplement in broilers instead of a feed ingredient. To the best of our knowledge, no report has shown the effect of a high-level incorporation (&gt;2\u2009% in the diet) of<jats:italic>C. vulgaris<\/jats:italic>on plasma metabolites and hepatic lipid composition of broilers. One hundred and twenty Ross 308 male birds were housed in 40 wired-floor cages and randomly distributed by the following experimental diets at 22 days of age (<jats:italic>n<\/jats:italic>\u2009=\u200910) during 15 days: (1) a corn-soybean meal based diet (control); (2) based diet with 10%\u00a0of<jats:italic>C. vulgaris<\/jats:italic>; (3) diet<jats:italic>2<\/jats:italic>supplemented with 0.005% Rovabio<jats:sup>\u00ae<\/jats:sup>Excel AP; and (4) diet<jats:italic>2<\/jats:italic>supplemented with 0.01% of a pre-selected four-CAZyme mixture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The inclusion of<jats:italic>C. vulgaris<\/jats:italic>at 10% in the diet, regardless of the presence of exogenous CAZymes, changed plasma metabolites but did not compromise broilers growth. Plasma total lipids increased in broilers fed<jats:italic>C. vulgaris<\/jats:italic>combined with the two feed CAZymes (<jats:italic>p<\/jats:italic>&lt; 0.001) compared with the control diet. Moreover, the supplementation with Rovabio<jats:sup>\u00ae<\/jats:sup>increased total cholesterol and LDL-cholesterol, while the addition of the four-CAZyme mixture increased triacylglycerols, VLDL-cholesterol and ALP activity. In opposition, HDL-cholesterol levels decreased in broilers fed microalga alone (<jats:italic>p<\/jats:italic>\u2009=\u20090.002). Regarding hepatic composition, the inclusion of<jats:italic>C. vulgaris<\/jats:italic>in broiler diets, individually or combined with exogenous CAZymes, had a minor effect on fatty acids but improved the<jats:italic>n<\/jats:italic>-6\/<jats:italic>n<\/jats:italic>-3 ratio and total carotenoids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, the inclusion of a high level (10%) of<jats:italic>C. vulgaris<\/jats:italic>in broiler\u00b4s diet, regardless of the presence of exogenous CAZymes, improved hepatic antioxidant composition and did not impair broiler\u2019s performance. In addition, the feed supplementation with CAZymes increased broilers lipemia. Therefore, dietary<jats:italic>C. vulgaris<\/jats:italic>at this incorporation level seems to be safe for animal health and do not compromise performance traits, with no need of CAZymes supplementation.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-021-02932-8","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T12:02:42Z","timestamp":1624968162000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Impact of dietary Chlorella vulgaris and carbohydrate-active enzymes incorporation on plasma metabolites and liver lipid composition of broilers"],"prefix":"10.1186","volume":"17","author":[{"given":"Diogo Francisco Maur\u00edcio","family":"Coelho","sequence":"first","affiliation":[]},{"given":"Cristina Maria Riscado Pereira Mate","family":"Alfaia","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Miguel Pestana","family":"Assun\u00e7\u00e3o","sequence":"additional","affiliation":[]},{"given":"M\u00f3nica","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Rui Manuel Amaro","family":"Pinto","sequence":"additional","affiliation":[]},{"given":"Carlos Mendes Godinho","family":"de Andrade Fontes","sequence":"additional","affiliation":[]},{"given":"Madalena M.","family":"Lordelo","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Ant\u00f3nio Mestre","family":"Prates","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,6,29]]},"reference":[{"key":"2932_CR1","doi-asserted-by":"publisher","first-page":"103889","DOI":"10.1016\/j.micpath.2019.103889","volume":"139","author":"A Guaragni","year":"2020","unstructured":"Guaragni A, Boiago MM, Bottari NB, Morsch VM, Lopes TF, da Silva AS. 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