{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:49:37Z","timestamp":1761130177234,"version":"3.37.3"},"reference-count":77,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2011\/02\/A\/NZ5\/00338"],"award-info":[{"award-number":["2011\/02\/A\/NZ5\/00338"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014434","name":"Narodowa Agencja Wymiany Akademickiej","doi-asserted-by":"publisher","award":["BPN\/BPT\/2021\/1\/00026\/U\/00001"],"award-info":[{"award-number":["BPN\/BPT\/2021\/1\/00026\/U\/00001"]}],"id":[{"id":"10.13039\/501100014434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Endometrosis (chronic degenerative endometritis) results in morphological changes in the equine endometrium and impairs its secretory function. However, the effect of this condition on the myometrium remains unclear. Lysophosphatidic acid (LPA) may affect female reproductive function and embryo transport by influencing uterine contractility through its receptors (LPARs). The objective of this study was to determine myometrial <jats:italic>LPAR1\u20136<\/jats:italic> mRNA transcription, and the effects of LPA on myometrial contractions in mares with endometrosis during the mid-luteal and follicular phases of the estrous cycle.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A reduction in myometrial <jats:italic>LPAR1<\/jats:italic> mRNA transcription was observed in mares with endometrosis during the mid-luteal phase, in comparison to those with category I endometria (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). While, upregulation of myometrial <jats:italic>LPAR3<\/jats:italic> or <jats:italic>LPAR6<\/jats:italic> mRNA transcription was observed in mares with category III or IIB endometria; respectively (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). An increase in myometrial <jats:italic>LPAR1<\/jats:italic>, <jats:italic>LPAR3<\/jats:italic> and <jats:italic>LPAR5<\/jats:italic> mRNA transcription was observed during the follicular phase in mares with category IIA endometrium in comparison to their expression in category I endometrium (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). During endometrosis progression LPA reduced the force of myometrial contractions in both phases of the estrous cycle (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). However, in mares with category IIA endometrium during the follicular phase, LPA was found to increase the force of contraction of myometrial strips in comparison to mares with category I endometrium (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>In the course of endometrosis in mares, a disruption in the myometrial mRNA transcription of <jats:italic>LPARs<\/jats:italic> has been observed. This is the first study to examine the impact of LPA on myometrial contractility at diffrent stage of endometrosis. However, it is essential to consider that multiple factors may contribute to this process. Alternations in contractile activity and changes in myometrial <jats:italic>LPARs<\/jats:italic> mRNA transcription may indicate impaired LPA-signaling mechanisms in equine myometrium during endometrosis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-024-04384-2","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T03:44:32Z","timestamp":1734579872000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The effect of lysophosphatidic acid on myometrial contractility and the mRNA transcription of its receptors in the myometrium at different stages of endometrosis in mares"],"prefix":"10.1186","volume":"20","author":[{"given":"Katarzyna Karolina","family":"Piotrowska-Tomala","sequence":"first","affiliation":[]},{"given":"Anna","family":"Sz\u00f3stek-Mioduchowska","sequence":"additional","affiliation":[]},{"given":"Agnieszka Walentyna","family":"Jonczyk","sequence":"additional","affiliation":[]},{"given":"Ewa Monika","family":"Drzewiecka","sequence":"additional","affiliation":[]},{"given":"Micha\u0142 Hubert","family":"Wrobel","sequence":"additional","affiliation":[]},{"given":"Takuo","family":"Hojo","sequence":"additional","affiliation":[]},{"given":"Graca","family":"Ferreira-Dias","sequence":"additional","affiliation":[]},{"given":"Dariusz Jan","family":"Skarzynski","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,12,19]]},"reference":[{"key":"4384_CR1","first-page":"241","volume":"172","author":"RM Kenney","year":"1978","unstructured":"Kenney RM. Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on early embryonic death. J Am Vet Med Assoc. 1978;172:241\u201362.","journal-title":"J Am Vet Med Assoc"},{"key":"4384_CR2","unstructured":"Kenney RM, Doig PA. Equine endometrial biopsy. In: Morrow DA, editor. Current Therapy in Theriogenology. Philadelphia: W.B. Saunders. 1986. p. 723\u201329."},{"key":"4384_CR3","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.anireprosci.2008.03.019","volume":"111","author":"C Hoffmann","year":"2009","unstructured":"Hoffmann C, Ellenberger C, Mattos RC, Aupperle H, Dhein S, Stief B, Schoon HA. The equine endometrosis: new insights into the pathogenesis. Anim Reprod Sci. 2009;111:261\u201378. https:\/\/doi.org\/10.1016\/j.anireprosci.2008.03.019.","journal-title":"Anim Reprod Sci"},{"key":"4384_CR4","doi-asserted-by":"publisher","first-page":"547","DOI":"10.2478\/pjvs-2014-0083","volume":"17","author":"J Buczkowska","year":"2014","unstructured":"Buczkowska J, Kozdrowski R, Nowak M, Ra\u015b A, Mrowiec J. Endometrosis-significance for horse reproduction, pathogenesis, diagnosis, and proposed therapeutic methods. Pol J Vet Sci. 2014;17:547\u201354. https:\/\/doi.org\/10.2478\/pjvs-2014-0083.","journal-title":"Pol J Vet Sci"},{"key":"4384_CR5","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1016\/j.theriogenology.2012.03.024","volume":"78","author":"AZ Sz\u00f3stek","year":"2012","unstructured":"Sz\u00f3stek AZ, Siemieniuch MJ, Lukasik K, Galv\u00e3o AM, Ferreira-Dias GM, Skarzynski DJ. mRNA transcription of prostaglandin synthases and their products in the equine endometrium in the course of fibrosis. Theriogenology. 2012;78:768\u201376. https:\/\/doi.org\/10.1016\/j.theriogenology.2012.03.024.","journal-title":"Theriogenology"},{"key":"4384_CR6","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1095\/biolreprod.113.10944735","volume":"89","author":"AZ Sz\u00f3stek","year":"2013","unstructured":"Sz\u00f3stek AZ, Lukasik K, Galv\u00e3o AM, Ferreira-Dias GM, Skarzynski DJ. Impairment of the interleukin system in equine endometrium during the course of endometrosis. Biol Reprod. 2013;89:79. https:\/\/doi.org\/10.1095\/biolreprod.113.10944735.","journal-title":"Biol Reprod"},{"key":"4384_CR7","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1095\/biolreprod65.5.1311","volume":"5","author":"CA Gray","year":"2016","unstructured":"Gray CA, Bartol FF, Tarleton BJ, Wiley AA, Johnson GA, Bazer FW. Developmental biology of uterine glands. Biol Reprod. 2016;5:1311\u201323. https:\/\/doi.org\/10.1095\/biolreprod65.5.1311.","journal-title":"Biol Reprod"},{"key":"4384_CR8","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.theriogenology.2018.10.005","volume":"124","author":"AZ Sz\u00f3stek-Mioduchowska","year":"2019","unstructured":"Sz\u00f3stek-Mioduchowska AZ, Lukasik K, Skarzynski DJ, Okuda K. Effect of transforming growth factor -\u03b21 on \u03b1-smooth muscle actin and collagen expression in equine endometrial fibroblasts. Theriogenology. 2019;124:9\u201317. https:\/\/doi.org\/10.1016\/j.theriogenology.2018.10.005.","journal-title":"Theriogenology"},{"key":"4384_CR9","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1038\/s41598-020-58109-0","volume":"10","author":"A Sz\u00f3stek-Mioduchowska","year":"2020","unstructured":"Sz\u00f3stek-Mioduchowska A, S\u0142owi\u0144ska M, Pacewicz J, Skarzynski DJ, Okuda K. Matrix metallopeptidase expression and modulation by transforming growth factor-\u03b21 in equine endometrosis. Sci Rep. 2020;10:1119. https:\/\/doi.org\/10.1038\/s41598-020-58109-0.","journal-title":"Sci Rep"},{"key":"4384_CR10","doi-asserted-by":"publisher","first-page":"16263","DOI":"10.1038\/s41598-023-43359-5","volume":"13","author":"A Sz\u00f3stek-Mioduchowska","year":"2023","unstructured":"Sz\u00f3stek-Mioduchowska A, W\u00f3jtowicz A, Sadowska A, Moza Jalali B, S\u0142yszewska M, \u0141ukasik K, Gurgul A, Szmato\u0142a T, Bugno-Poniewierska M, Ferreira-Dias G, Skarzynski DJ. Transcriptomic profiling of mare endometrium at different stages of endometrosis. Sci Rep. 2023;13:16263. https:\/\/doi.org\/10.1038\/s41598-023-43359-5.","journal-title":"Sci Rep"},{"key":"4384_CR11","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1294\/jes.25.45","volume":"25","author":"M Hanada","year":"2014","unstructured":"Hanada M, Maeda Y, Oikawa MA. Histopathological Characteristics of Endometrosis in Thoroughbred Mares in Japan: Results from 50 Necropsy Cases. J Equine Sci. 2014;25:45\u201352. https:\/\/doi.org\/10.1294\/jes.25.45.","journal-title":"J Equine Sci"},{"issue":"52","key":"4384_CR12","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1093\/biolreprod\/52.monograph_series1.501","volume":"1","author":"MM LeBlanc","year":"1995","unstructured":"LeBlanc MM, Johnson RD, Calderwood-Mays MB, Valderrama C. Lymphatic clearance of India ink in reproductively normal mares and mares susceptible to endometritis. Biol Reprod Monogr Ser. 1995;1(52):501\u20136. https:\/\/doi.org\/10.1093\/biolreprod\/52.monograph_series1.501.","journal-title":"Biol Reprod Monogr Ser"},{"key":"4384_CR13","first-page":"283","volume":"44","author":"MHT Troedsson","year":"1991","unstructured":"Troedsson MHT, Liu IKM. Uterine clearance of non-antigenic markers (51Cr) in response to a bacterial challenge in mares potentially susceptible and resistant to chronic uterine infections. J Reprod Fertil Suppl. 1991;44:283\u20138.","journal-title":"J Reprod Fertil Suppl"},{"key":"4384_CR14","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1530\/jrf.0.0990307","volume":"99","author":"MHT Troedsson","year":"1993","unstructured":"Troedsson MHT, Liu IKM, Ing M, Pascoe J, Thurmond M. Multiple site electromyography recordings of uterine activity following an intrauterine bacterial challenge in mares susceptible and resistant to chronic uterine infection. J Reprod Fertil. 1993;99:307\u201313. https:\/\/doi.org\/10.1530\/jrf.0.0990307.","journal-title":"J Reprod Fertil"},{"key":"4384_CR15","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/s0014-5793(97)00411-0","volume":"410","author":"F Gaits","year":"1997","unstructured":"Gaits F, Fourcade O, Le Balle F, Gueguen G, Gaige B, Gassama-Diagne A, Fauvel J, Salles JP, Mauco G, Simon MF, Chap H. Lysophosphatidic acid as a phospholipid mediator: pathways of synthesis. FEBS Lett. 1997;410:54\u20138. https:\/\/doi.org\/10.1016\/s0014-5793(97)00411-0.","journal-title":"FEBS Lett"},{"key":"4384_CR16","doi-asserted-by":"publisher","first-page":"25600","DOI":"10.1074\/jbc.M302648200","volume":"278","author":"K Noguchi","year":"2003","unstructured":"Noguchi K, Ishii S, Shimizu T. Identification of p2y 9\/GPR23 as a novel G protein-coupled receptor for Lysophosphatidic acid, structurally distant from the Edg family. J Biol Chem. 2003;278:25600\u20136. https:\/\/doi.org\/10.1074\/jbc.M302648200.","journal-title":"J Biol Chem"},{"key":"4384_CR17","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.semcdb.2004.05.005","volume":"15","author":"B Anliker","year":"2004","unstructured":"Anliker B, Chun J. Cell surface receptors in lysophospholipid signaling. Semin Cell Dev Biol. 2004;15:457\u201365. https:\/\/doi.org\/10.1016\/j.semcdb.2004.05.005.","journal-title":"Semin Cell Dev Biol"},{"key":"4384_CR18","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/j.semcdb.2004.05.001","volume":"15","author":"J Aoki","year":"2004","unstructured":"Aoki J. Mechanisms of lysophosphatidic acid production. Semin Cell Dev Biol. 2004;15:477\u201389. https:\/\/doi.org\/10.1016\/j.semcdb.2004.05.001.","journal-title":"Semin Cell Dev Biol"},{"key":"4384_CR19","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.prostaglandins.2009.02.002","volume":"91","author":"M Lin","year":"2010","unstructured":"Lin M, Herr DR, Chun J. Lysophosphatidic acid (LPA) receptors: signaling properties and disease relevance. Prostaglandins Other Lipid Mediat. 2010;91:130\u20138. https:\/\/doi.org\/10.1016\/j.prostaglandins.2009.02.002.","journal-title":"Prostaglandins Other Lipid Mediat"},{"key":"4384_CR20","doi-asserted-by":"publisher","first-page":"1192","DOI":"10.1194\/jlr.R046458","volume":"55","author":"YC Yung","year":"2014","unstructured":"Yung YC, Stoddard NC, Chun J. LPA receptor signaling: pharmacology, physiology, and pathophysiology. J Lipid Res. 2014;55:1192\u2013214. https:\/\/doi.org\/10.1194\/jlr.R046458.","journal-title":"J Lipid Res"},{"key":"4384_CR21","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.plipres.2007.02.001","volume":"46","author":"LA van Meeteren","year":"2007","unstructured":"van Meeteren LA, Moolenaar WH. Regulation and biological activities of the autotaxin-LPA axis. Prog Lipid Res. 2007;46:145\u201360. https:\/\/doi.org\/10.1016\/j.plipres.2007.02.001.","journal-title":"Prog Lipid Res"},{"key":"4384_CR22","first-page":"74","volume":"245","author":"A Tokumura","year":"1980","unstructured":"Tokumura A, Fukuzawa K, Yamada S, Tsukatani H. Stimulatory effect of lysophosphatidic acids on uterine smooth muscles of non-pregnant rats. Arch Int Pharmacodyn Ther. 1980;245:74\u201383.","journal-title":"Arch Int Pharmacodyn Ther"},{"issue":"11","key":"4384_CR23","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1292\/jvms.23-0336","volume":"85","author":"S Kurusu","year":"2023","unstructured":"Kurusu S, Terashima R, Sugiyama M, Tanaka M, Kadowaki T, Kizaki K, Kawaminami M. Expression of lysophosphatidic acid receptors in the rat uterus: cellular distribution of protein and gestation-associated changes in gene expression. J Vet Med Sci. 2023;85(11):1165\u201371. https:\/\/doi.org\/10.1292\/jvms.23-0336.","journal-title":"J Vet Med Sci"},{"key":"4384_CR24","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1038\/nature03505","volume":"435","author":"X Ye","year":"2005","unstructured":"Ye X, Hama K, Contos JJ, Anliker B, Inoue A, Skinner MK, Suzuki H, Amano T, Kennedy G, Arai H, Aoki J, Chun J. LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing. Nature. 2005;435:104\u20138. https:\/\/doi.org\/10.1038\/nature03505.","journal-title":"Nature"},{"key":"4384_CR25","doi-asserted-by":"publisher","first-page":"6166","DOI":"10.1210\/en.2008-0354","volume":"149","author":"H Seo","year":"2008","unstructured":"Seo H, Kim M, Choi Y, Lee CK, Ka H. Analysis of lysophosphatidic acid (LPA) receptor and LPA-induced endometrial prostaglandin-endoperoxide synthase 2 expression in the porcine uterus. Endocrinology. 2008;149:6166\u201375. https:\/\/doi.org\/10.1210\/en.2008-0354.","journal-title":"Endocrinology"},{"key":"4384_CR26","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1530\/REP-08-0209","volume":"137","author":"I Woclawek-Potocka","year":"2009","unstructured":"Woclawek-Potocka I, Komiyama J, Saulnier-Blache JS, Brzezicka E, Bah MM, Okuda K, Skarzynski DJ. Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus. Reproduction. 2009;137:95\u2013105. https:\/\/doi.org\/10.1530\/REP-08-0209.","journal-title":"Reproduction"},{"key":"4384_CR27","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1262\/jrd.09-205","volume":"56","author":"I Woclawek-Potocka","year":"2010","unstructured":"Woclawek-Potocka I, Kowalczyk-Zieba I, Skarzynski DJ. Lysophosphatidic acid action during early pregnancy in the cow: in vivo and in vitro studies. J Reprod Dev. 2010;56:411\u201320. https:\/\/doi.org\/10.1262\/jrd.09-205.","journal-title":"J Reprod Dev"},{"issue":"1","key":"4384_CR28","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.repbio.2013.01.166","volume":"13","author":"D Boruszewska","year":"2013","unstructured":"Boruszewska D, Kowalczyk-Zieba I, Piotrowska-Tomala K, Saulnier-Blache JS, Acosta T, Skarzynski DJ, Woclawek-Potocka I. Which bovine endometrial cells are the source of and target for lysophosphatidic acid? Reprod Biol. 2013;13(1):100\u20133. https:\/\/doi.org\/10.1016\/j.repbio.2013.01.166.","journal-title":"Reprod Biol"},{"key":"4384_CR29","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.repbio.2013.09.004","volume":"13","author":"D Boruszewska","year":"2013","unstructured":"Boruszewska D, Sinderewicz E, Kowalczyk-Zieba I, Skarzynski DJ, Woclawek-Potocka I. Influence of lysophosphatidic acid on estradiol production and follicle stimulating hormone action in bovine granulosa cells. Reprod Biol. 2013;13:344\u20137.","journal-title":"Reprod Biol"},{"key":"4384_CR30","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1210\/en.2008-0749","volume":"150","author":"E Liszewska","year":"2009","unstructured":"Liszewska E, Reinaud P, Billon-Denis E, Dubois O, Robin P, Charpigny G. Lysophosphatidic acid signaling during embryo development in sheep: involvement in prostaglandin synthesis. Endocrinology. 2009;150:422\u201334. https:\/\/doi.org\/10.1210\/en.2008-0749.","journal-title":"Endocrinology"},{"key":"4384_CR31","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.domaniend.2011.08.003","volume":"42","author":"E Liszewska","year":"2012","unstructured":"Liszewska E, Reinaud P, Dubois O, Charpigny G. Lysophosphatidic acid receptors in ovine uterus during estrous cycle and early pregnancy and their regulation by progesterone. Domest Anim Endocrinol. 2012;42:31\u201342. https:\/\/doi.org\/10.1016\/j.domaniend.2011.08.003.","journal-title":"Domest Anim Endocrinol"},{"key":"4384_CR32","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1095\/biolreprod65.2.401","volume":"65","author":"W Gogarten","year":"2001","unstructured":"Gogarten W, Emala CW, Lindeman KS, Hirshman CA. Oxytocin and Lysophosphatidic Acid Induce Stress Fiber Formation in Human Myometrial Cells via a Pathway Involving Rho-Kinase. Biol Reprod. 2001;65:401\u20136. https:\/\/doi.org\/10.1095\/biolreprod65.2.401.","journal-title":"Biol Reprod"},{"key":"4384_CR33","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1262\/jrd.2023-011","volume":"69","author":"S Nagashima","year":"2023","unstructured":"Nagashima S, Kimura T, Terashima R, Sugiyama M, Kizaki K, Kawaminami M, Kurusu S. Lysophosphatidic acid stimulates rat uterine contraction in vitro. J Reprod Dev. 2023;69:163\u20139. https:\/\/doi.org\/10.1262\/jrd.2023-011.","journal-title":"J Reprod Dev"},{"key":"4384_CR34","doi-asserted-by":"publisher","first-page":"635","DOI":"10.2478\/v10181-012-0100-9","volume":"15","author":"W Markiewicz","year":"2012","unstructured":"Markiewicz W, Kami\u0144ska K, Bogacki M, Ma\u015blanka T, Jaroszewski J. Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-\u03b1-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs. Pol J Vet Sci. 2012;15:635\u201343. https:\/\/doi.org\/10.2478\/v10181-012-0100-9.","journal-title":"Pol J Vet Sci"},{"key":"4384_CR35","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1016\/j.theriogenology.2007.04.011","volume":"68","author":"RC Causey","year":"2007","unstructured":"Causey RC. Mucus and the mare: How little we know. Theriogenology. 2007;68:386\u201394. https:\/\/doi.org\/10.1016\/j.theriogenology.2007.04.011.","journal-title":"Theriogenology"},{"key":"4384_CR36","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1111\/rda.13013","volume":"52","author":"M Christoffersen","year":"2017","unstructured":"Christoffersen M, Troedsson M. Inflammation and fertility in the mare. Reprod Domest Anim. 2017;52:4\u201320. https:\/\/doi.org\/10.1111\/rda.13013.","journal-title":"Reprod Domest Anim"},{"key":"4384_CR37","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1530\/jrf.0.0870331","volume":"87","author":"ED Watson","year":"1989","unstructured":"Watson ED, Sertich PL. Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares. J Reprod Fertil. 1989;87:331\u20136. https:\/\/doi.org\/10.1530\/jrf.0.0870331.","journal-title":"J Reprod Fertil"},{"key":"4384_CR38","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/j.theriogenology.2005.05.048","volume":"65","author":"G Hirsbrunner","year":"2006","unstructured":"Hirsbrunner G, Reist M, Couto SS, Steiner A, Snyder J, Vanleeuwen E, Liu I. An in vitro study on spontaneous myometrial contractility in the mare during estrus and diestrus. Theriogenology. 2006;65:517\u201327. https:\/\/doi.org\/10.1016\/j.theriogenology.2005.05.048.","journal-title":"Theriogenology"},{"key":"4384_CR39","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1093\/humupd\/dmq016","volume":"16","author":"HN Aguilar","year":"2010","unstructured":"Aguilar HN, Mitchell BF. Physiological pathways and molecular mechanisms regulating uterine contractility. Hum Reprod Update. 2010;16:725\u201344. https:\/\/doi.org\/10.1093\/humupd\/dmq016.","journal-title":"Hum Reprod Update"},{"key":"4384_CR40","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1186\/s12917-020-02562-6","volume":"16","author":"A Sz\u00f3stek-Mioduchowska","year":"2020","unstructured":"Sz\u00f3stek-Mioduchowska A, Leciejewska N, Zelma\u0144ska B, Staszkiewicz-Chodor J, Ferreira-Dias G, Skarzynski D. Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the mare. BMC Vet Res. 2020;16:343. https:\/\/doi.org\/10.1186\/s12917-020-02562-6.","journal-title":"BMC Vet Res"},{"key":"4384_CR41","doi-asserted-by":"publisher","unstructured":"Roberto da Costa RP, Serr\u00e3o PM, Monteiro S, Pessa P, Silva JR, Ferreira-Dias G. Caspase-3-mediated apoptosis and cell proliferation in the equine endometrium during the oestrous cycle. Reprod Fertil Dev. 2007;19: 925\u2013932. https:\/\/doi.org\/10.1071\/rd06159.","DOI":"10.1071\/rd06159"},{"key":"4384_CR42","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.taap.2015.03.005","volume":"285","author":"MH Wrobel","year":"2015","unstructured":"Wrobel MH, Grzeszczyk M, M\u0142ynarczuk J, Kotwica J. The adverse effects of aldrin and dieldrin on both myometrial contractions and the secretory functions of bovine ovaries and uterus in vitro. Toxicol Appl Pharmacol. 2015;285:23\u201331. https:\/\/doi.org\/10.1016\/j.taap.2015.03.005.","journal-title":"Toxicol Appl Pharmacol"},{"key":"4384_CR43","doi-asserted-by":"publisher","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","volume":"64","author":"C Andersen","year":"2004","unstructured":"Andersen C, Jensen JL, \u00d8rntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004;64:5245\u201350. https:\/\/doi.org\/10.1158\/0008-5472.CAN-04-0496.","journal-title":"Cancer Res"},{"key":"4384_CR44","first-page":"303","volume":"5","author":"M Wrobel","year":"2005","unstructured":"Wrobel M, Kaminski K, Kotwica J. In vitro effects of polychlorinated biphenyls (PCBs) on the contractility of bovine myometrium from the periovulatory stage of the estrous cycle. Reprod Biol. 2005;5:303\u201319.","journal-title":"Reprod Biol"},{"key":"4384_CR45","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.tox.2009.06.012","volume":"262","author":"MH Wrobel","year":"2009","unstructured":"Wrobel MH, Rekawiecki R, Kotwica J. Involvement of prostaglandin F2\u03b1 in the adverse effect of PCB 77 on the force of contractions of bovine myometrium. Toxicology. 2009;262:224\u20139. https:\/\/doi.org\/10.1016\/j.tox.2009.06.012.","journal-title":"Toxicology"},{"key":"4384_CR46","doi-asserted-by":"publisher","first-page":"1698","DOI":"10.1016\/j.theriogenology.2011.12.015","volume":"77","author":"AZ Sz\u00f3stek","year":"2012","unstructured":"Sz\u00f3stek AZ, Siemieniuch MJ, Galv\u00e3o AM, Lukasik K, Zieba D, Ferreira-Dias GM, Skarzynski DJ. Effects of cell storage and passage on basal and oxytocin-regulated prostaglandin secretion by equine endometrial epithelial and stromal cells. Theriogenology. 2012;77:1698\u2013708. https:\/\/doi.org\/10.1016\/j.theriogenology.2011.12.015.","journal-title":"Theriogenology"},{"key":"4384_CR47","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1016\/S0093-691X(03)00086-4","volume":"60","author":"G Kotwica","year":"2003","unstructured":"Kotwica G, Kurowicka B, Franczaka A, Grzegorzewski W, Wrobel M, Mlynarczuk J, Kotwica J. The concentrations of catecholamines and oxytocin receptors in the oviduct and its contractile activity in cows during the estrous cycle. Theriogenology. 2003;60:953\u201364.","journal-title":"Theriogenology"},{"key":"4384_CR48","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.tvjl.2013.09.021","volume":"199","author":"AJ Korzekwa","year":"2014","unstructured":"Korzekwa AJ, Lukasik K, Pilawski W, Piotrowska-Tomala KK, Jaroszewski JJ, Yoshioka S, Okuda K, Skarzynski DJ. Influence of prostaglandin F\u2082\u03b1 analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro. Vet J. 2014;199:131\u20137. https:\/\/doi.org\/10.1016\/j.tvjl.2013.09.021.","journal-title":"Vet J"},{"key":"4384_CR49","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1038\/s41392-020-00367-5","volume":"6","author":"LHM Geraldo","year":"2021","unstructured":"Geraldo LHM, Spohr TCLdS, Amaral RFd, Fonseca ACCd, Garcia C, Mendes FdA, dosSantos MF, Lima FRS. Role of lysophosphatidic acid and its receptors in health and disease: novel therapeutic strategies. Sig Transduct Target Ther. 2021;6:45.","journal-title":"Sig Transduct Target Ther"},{"key":"4384_CR50","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.prostaglandins.2007.10.001","volume":"85","author":"K Kaminska","year":"2008","unstructured":"Kaminska K, Wasielak M, Bogacka I, Blitek M, Bogacki M. Quantitative expression of lysophosphatidic acid receptor 3 gene in porcine endometrium during the periimplantation period and estrous cycle. Prostaglandins Other Lipid Mediat. 2008;85:26\u201332. https:\/\/doi.org\/10.1016\/j.prostaglandins.2007.10.001.","journal-title":"Prostaglandins Other Lipid Mediat"},{"key":"4384_CR51","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1262\/jrd.2012-060","volume":"58","author":"I Kowalczyk-Zieba","year":"2012","unstructured":"Kowalczyk-Zieba I, Boruszewska D, Saulnier-Blache JS, Da Costa LL, Jankowska K, Skarzynski DJ, Woclawek-Potocka I. Lysophosphatidic acid action in the bovine corpus luteum\u2014an in vitro study. J Reprod Dev. 2012;58:661\u201371. https:\/\/doi.org\/10.1262\/jrd.2012-060.","journal-title":"J Reprod Dev"},{"key":"4384_CR52","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.theriogenology.2017.05.019","volume":"87","author":"A Sadam","year":"2017","unstructured":"Sadam A, Parida S, Padol AR, Verma AD, Baba NA, Khuman WM, Srivastava V, Panigrahi M, Singh TU, Sarkar SN. Study of lysophosphatidic acid receptors (LPARs) in buffalo uterus demonstrated upregulation of LPAR1 and LPAR6 in early pregnancy. Theriogenology. 2017;87:90\u20137. https:\/\/doi.org\/10.1016\/j.theriogenology.2017.05.019.","journal-title":"Theriogenology"},{"key":"4384_CR53","doi-asserted-by":"publisher","first-page":"3871","DOI":"10.1242\/jcs.172098","volume":"128","author":"H Yukiura","year":"2015","unstructured":"Yukiura H, Kano K, Kise R, Inoue A, Aoki J. LPP3 localizes LPA6 signalling to non-contact sites in endothelial cells. J Cell Sci. 2015;128:3871\u20137. https:\/\/doi.org\/10.1242\/jcs.172098.","journal-title":"J Cell Sci"},{"key":"4384_CR54","doi-asserted-by":"publisher","first-page":"1736","DOI":"10.1016\/j.lfs.2006.06.002","volume":"79","author":"K Hama","year":"2006","unstructured":"Hama K, Aoki J, Bandoh K, Inoue A, Endo T, Amano T, Suzuki H, Arai H. Lysophosphatidic receptor, LPA3, is positively and negatively regulated by progesterone and estrogen in the mouse uterus. Life Sci. 2006;79:1736\u201340.","journal-title":"Life Sci"},{"key":"4384_CR55","doi-asserted-by":"publisher","first-page":"2107","DOI":"10.1016\/j.fertnstert.2011.02.024","volume":"95","author":"X Ye","year":"2011","unstructured":"Ye X, Herr DR, Diao H, Rivera R, Chun J. Unique uterine localization and regulation may differentiate LPA3 from other lysophospholipid receptors for its role in embryo implantation. Fertil Steril. 2011;95:2107\u201313.","journal-title":"Fertil Steril"},{"issue":"Suppl 3","key":"4384_CR56","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1111\/j.1439-0531.2009.01485.x","volume":"44","author":"MM LeBlanc","year":"2009","unstructured":"LeBlanc MM, Causey RC. Clinical and subclinical endometritis in the mare: both threats to fertility. Reprod Domest Anim. 2009;44(Suppl 3):10\u201322. https:\/\/doi.org\/10.1111\/j.1439-0531.2009.01485.x.","journal-title":"Reprod Domest Anim"},{"key":"4384_CR57","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.3390\/ijms21041432","volume":"21","author":"IF Canisso","year":"2020","unstructured":"Canisso IF, Segabinazzi LGTM, Fedorka CE. Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology. Int J Mol Sci. 2020;21:1432. https:\/\/doi.org\/10.3390\/ijms21041432.","journal-title":"Int J Mol Sci"},{"key":"4384_CR58","doi-asserted-by":"publisher","first-page":"779","DOI":"10.3390\/ani12060779","volume":"12","author":"T Katila","year":"2022","unstructured":"Katila T, Ferreira-Dias G. Evolution of the Concepts of Endometrosis, Post Breeding Endometritis, and Susceptibility of Mares. Animals (Basel). 2022;12:779. https:\/\/doi.org\/10.3390\/ani12060779.","journal-title":"Animals (Basel)"},{"key":"4384_CR59","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.theriogenology.2020.01.018","volume":"150","author":"DJ Skarzynski","year":"2020","unstructured":"Skarzynski DJ, Sz\u00f3stek-Mioduchowska AZ, Rebord\u00e3o MR, Jalali BM, Piotrowska-Tomala KK, Leciejewska N, \u0141azarczyk M, Ferreira-Dias GM. Neutrophils, monocytes and other immune components in the equine endometrium: Friends or foes? Theriogenology. 2020;150:150\u20137. https:\/\/doi.org\/10.1016\/j.theriogenology.2020.01.018.","journal-title":"Theriogenology"},{"key":"4384_CR60","doi-asserted-by":"publisher","first-page":"2615","DOI":"10.3390\/ani11092615","volume":"11","author":"MR Rebord\u00e3o","year":"2021","unstructured":"Rebord\u00e3o MR, Amaral A, Fernandes C, Silva E, Lukasik K, Sz\u00f3stek-Mioduchowska A, Pinto-Bravo P, Galv\u00e3o A, Skarzynski DJ, Ferreira-Dias G. Enzymes Present in Neutrophil Extracellular Traps May Stimulate the Fibrogenic PGF2\u03b1 Pathway in the Mare Endometrium. Animals (Basel). 2021;11:2615. https:\/\/doi.org\/10.3390\/ani11092615.","journal-title":"Animals (Basel)"},{"key":"4384_CR61","first-page":"474","volume":"32","author":"RK Patil","year":"1980","unstructured":"Patil RK, Sinha SN, Einarsson S, Settergren I. The effect of prostaglandin F2 alpha and oxytocin on bovine myometrium in vitro. Nord Vet Med. 1980;32:474\u20139.","journal-title":"Nord Vet Med"},{"key":"4384_CR62","first-page":"681","volume":"56","author":"JC Ousey","year":"2000","unstructured":"Ousey JC, Freestone N, Fowden AL, Mason WT, Rossdale PD. The effects of oxytocin and progestagens on myometrial contractility in vitro during equine pregnancy. J Reprod Fertil Suppl. 2000;56:681\u201391.","journal-title":"J Reprod Fertil Suppl"},{"key":"4384_CR63","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1095\/biolreprod65.3.740","volume":"65","author":"SL Rigby","year":"2001","unstructured":"Rigby SL, Barhoumi R, Burghardt RC, Colleran P, Thompson JA, Varner DD, Blanchard TL, Brinsko SP, Taylor T, Wilkerson MK, Delp MD. Mares with delayed uterine clearance have an intrinsic defect in myometrial function. Biol Reprod. 2001;65:740\u20137. https:\/\/doi.org\/10.1095\/biolreprod65.3.740.","journal-title":"Biol Reprod"},{"key":"4384_CR64","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1095\/biolreprod.107.060293","volume":"77","author":"K Hama","year":"2007","unstructured":"Hama K, Aoki J, Inoue A, Endo T, Amano T, Motoki R, Kanai M, Ye X, Chun J, Matsuki N, Suzuki H, Shibasaki M, Arai H. Embryo spacing and implantation timing are differentially regulated by LPA3-mediated lysophosphatidic acid signaling in mice. Biol Reprod. 2007;77:954\u20139. https:\/\/doi.org\/10.1095\/biolreprod.107.060293.","journal-title":"Biol Reprod"},{"key":"4384_CR65","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1152\/jappl.1997.83.4.1216","volume":"83","author":"ML Toews","year":"1997","unstructured":"Toews ML, Ustinova EE, Schultz HD. Lysophosphatidic acid enhances contractility of isolated airway smooth muscle. J Appl Physiol. 1997;83:1216\u201322. https:\/\/doi.org\/10.1152\/jappl.1997.83.4.1216.","journal-title":"J Appl Physiol"},{"key":"4384_CR66","doi-asserted-by":"publisher","unstructured":"Stoddard NC, Chun J. Promising pharmacological directions in the world of lysophosphatidic Acid signaling. Biomol. Ther. (Seoul.). 2015; 23:1\u201311. https:\/\/doi.org\/10.4062\/biomolther.2014.109","DOI":"10.4062\/biomolther.2014.109"},{"key":"4384_CR67","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1146\/annurev.pharmtox.010909.105753","volume":"50","author":"JW Choi","year":"2010","unstructured":"Choi JW, Herr DR, Noguchi K, Yung YC, Lee CW, Mutoh T, Lin ME, Teo ST, Park KE, Mosley AN, Chun J. LPA Receptors: Subtypes and Biological Actions. Annu Rev Pharmacol Toxicol. 2010;50:157\u201386. https:\/\/doi.org\/10.1146\/annurev.pharmtox.010909.105753.","journal-title":"Annu Rev Pharmacol Toxicol"},{"key":"4384_CR68","doi-asserted-by":"publisher","first-page":"41085","DOI":"10.1074\/jbc.M404045200","volume":"279","author":"R Cummings","year":"2004","unstructured":"Cummings R, Zhao Y, Jacoby D, Spannhake EW, Ohba M, Garcia JG, Watkins T, He D, Saatian B, Natarajan V. Protein kinase Cdelta mediates lysophosphatidic acid-induced NF-kappaB activation and interleukin-8 secretion in human bronchial epithelial cells. J Biol Chem. 2004;279:41085\u201394.","journal-title":"J Biol Chem"},{"key":"4384_CR69","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1055\/s-0038-1666902","volume":"42","author":"KA Schumacher","year":"1979","unstructured":"Schumacher KA, Classen HG, Spath M. Platelet aggregation evoked in vitro and in vivo by phosphatidic acids and lysoderivatives: identity with substances in aged serum (DAS). Thromb Haemost. 1979;42:631\u201340.","journal-title":"Thromb Haemost"},{"key":"4384_CR70","first-page":"2076","volume":"25","author":"Y Zhao","year":"2013","unstructured":"Zhao Y, Natarajan V. Lysophosphatidic acid signaling in airway epithelium: Role in airway inflammation and remodeling. Cell Signal. 2013;25:2076\u201383.","journal-title":"Cell Signal"},{"key":"4384_CR71","first-page":"241","volume":"159","author":"G Tigyi","year":"2010","unstructured":"Tigyi G. Aiming drug discovery at lysophosphatidic acid targets Br. J Pharmacol. 2010;159:241\u201370.","journal-title":"J Pharmacol"},{"key":"4384_CR72","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.theriogenology.2020.04.039","volume":"153","author":"KK Piotrowska-Tomala","year":"2020","unstructured":"Piotrowska-Tomala KK, Jonczyk AW, Skarzynski DJ, Sz\u00f3stek-Mioduchowska AZ. Luteinizing hormone and ovarian steroids affect in vitro prostaglandin production in the equine myometrium and endometrium. Theriogenology. 2020;153:1\u20138. https:\/\/doi.org\/10.1016\/j.theriogenology.2020.04.039.","journal-title":"Theriogenology"},{"key":"4384_CR73","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1096\/fj.201600735R","volume":"31","author":"PT Dancs","year":"2017","unstructured":"Dancs PT, Ruisanchez \u00c9, Balogh A, Panta CR, Mikl\u00f3s Z, N\u00fcsing RM, Aoki J, Chun J, Offermanns S, Tigyi G, Beny\u00f3 Z. LPA1 receptor-mediated thromboxane A2 release is responsible for lysophosphatidic acid-induced vascular smooth muscle contraction. FASEB J. 2017;31:1547\u201355. https:\/\/doi.org\/10.1096\/fj.201600735R.","journal-title":"FASEB J"},{"key":"4384_CR74","doi-asserted-by":"publisher","first-page":"988","DOI":"10.1016\/j.theriogenology.2014.11.032","volume":"83","author":"A Esteller-Vico","year":"2015","unstructured":"Esteller-Vico A, Liu IKM, Vaughan B, Steffey EP, Brosnan RJ. Effects of vascular elastosis on uterine blood flow and perfusion in anesthetized mares. Theriogenology. 2015;83:988\u201394.","journal-title":"Theriogenology"},{"key":"4384_CR75","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.anireprosci.2015.11.012","volume":"164","author":"A Esteller-Vico","year":"2016","unstructured":"Esteller-Vico A, Liu IKM, Vaughan B, Steffey EP, Brosnan RJ. Effects of estradiol on uterine perfusion in anesthetized cyclic mares affected with uterine vascular elastosis. Anim Reprod Sci. 2016;164:57\u201363.","journal-title":"Anim Reprod Sci"},{"key":"4384_CR76","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1111\/j.2042-3306.1991.tb02752.x","volume":"23","author":"SW Ricketts","year":"1991","unstructured":"Ricketts SW, Alonso S. The effect of age and parity on the development of equine chronic endometrial disease. Equine Vet J. 1991;23:189\u201392. https:\/\/doi.org\/10.1111\/j.2042-3306.1991.tb02752.x.","journal-title":"Equine Vet J"},{"key":"4384_CR77","unstructured":"Ebert A, Schoon D, Schoon HA. Age-related endometrial alterations in mares\u2014biopsy findings of the last 20 years. In Leipziger Blaue Hefte, 7th Leipzig Veterinary Congress, 8th International Conference on Equine Reproductive Medicine; Rackwitz R, Pees M, Aschenbach, JR, G\u00e4bel G, Eds.; Lehmanns Media GmbH: Berlin, Germany, 2014; 2: 230\u2013232."}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-024-04384-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-024-04384-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-024-04384-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T05:03:56Z","timestamp":1734584636000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-024-04384-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,19]]},"references-count":77,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["4384"],"URL":"https:\/\/doi.org\/10.1186\/s12917-024-04384-2","relation":{},"ISSN":["1746-6148"],"issn-type":[{"type":"electronic","value":"1746-6148"}],"subject":[],"published":{"date-parts":[[2024,12,19]]},"assertion":[{"value":"28 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All material collection procedures were approved by the Local Ethics Committee for Experiments on Animals in Olsztyn, Poland (Agreements No. 51\/2011).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"571"}}