{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T11:46:14Z","timestamp":1767008774743,"version":"3.37.3"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/51602\/2011"],"award-info":[{"award-number":["SFRH\/BD\/51602\/2011"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"VENOMICS","award":["278346","278346","278346","278346","278346"],"award-info":[{"award-number":["278346","278346","278346","278346","278346"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s12934-016-0618-0","type":"journal-article","created":{"date-parts":[[2017,1,3]],"date-time":"2017-01-03T20:58:37Z","timestamp":1483477117000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli"],"prefix":"10.1186","volume":"16","author":[{"given":"Ana Filipa","family":"Sequeira","sequence":"first","affiliation":[]},{"given":"Jeremy","family":"Turchetto","sequence":"additional","affiliation":[]},{"given":"Natalie J.","family":"Saez","sequence":"additional","affiliation":[]},{"given":"Fanny","family":"Peysson","sequence":"additional","affiliation":[]},{"given":"Laurie","family":"Ramond","sequence":"additional","affiliation":[]},{"given":"Yoan","family":"Duhoo","sequence":"additional","affiliation":[]},{"given":"Marilyne","family":"Bl\u00e9mont","sequence":"additional","affiliation":[]},{"given":"V\u00e2nia O.","family":"Fernandes","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds T.","family":"Gama","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds M. A.","family":"Ferreira","sequence":"additional","affiliation":[]},{"given":"Catarina I. P. I.","family":"Guerreiro","sequence":"additional","affiliation":[]},{"given":"Nicolas","family":"Gilles","sequence":"additional","affiliation":[]},{"given":"Herv\u00e9","family":"Darbon","sequence":"additional","affiliation":[]},{"given":"Carlos M. G. A.","family":"Fontes","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9667-0196","authenticated-orcid":false,"given":"Renaud","family":"Vincentelli","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2017,1,17]]},"reference":[{"key":"618_CR1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1586\/epr.09.45","volume":"6","author":"P Escoubas","year":"2009","unstructured":"Escoubas P, King GF. Venomics as a drug discovery platform. Expert Rev Proteom. 2009;6:221\u20134.","journal-title":"Expert Rev Proteom."},{"key":"618_CR2","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/nrd1197","volume":"2","author":"RJ Lewis","year":"2003","unstructured":"Lewis RJ, Garcia ML. Therapeutic potential of venom peptides. Nat Rev Drug Discov. 2003;2:790\u2013802.","journal-title":"Nat Rev Drug Discov"},{"key":"618_CR3","first-page":"23","volume":"2","author":"MF Fernandes-Pedrosa","year":"2013","unstructured":"Fernandes-Pedrosa MF, F\u00e9lix-Silva J, Menezes Y. Toxins from venomous animals: gene cloning, protein expression and biotechnological applications. Integr View Mol Recognit Toxinologu Anal Proced Biomed Appl. 2013;2:23\u201372.","journal-title":"Integr View Mol Recognit Toxinologu Anal Proced Biomed Appl."},{"key":"618_CR4","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/B978-0-12-385120-8.00003-6","volume":"498","author":"M Welch","year":"2011","unstructured":"Welch M, Villalobos A, Gustafsson C, Minshull J. Designing genes for successful protein expression. Methods Enzymol. 2011;498:43\u201366.","journal-title":"Methods Enzymol"},{"key":"618_CR5","first-page":"2047","volume-title":"Escherichia coli Salmonella typhimurium: cellular and molecular biology","author":"A Henaut","year":"1996","unstructured":"Henaut A, Danchin A. Analysis and predictions from Escherichia coli sequences. In: Neidhart FC, Curtiss RI, Ingraham J, Lin E, Brooks Low K, editors. Escherichia coli Salmonella typhimurium: cellular and molecular biology. Washington DC: ASM Press; 1996. p. 2047."},{"key":"618_CR6","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0378-1119(03)00829-1","volume":"320","author":"A Fuglsang","year":"2003","unstructured":"Fuglsang A. The effective number of codons for individual amino acids: some codons are more optimal than others. Gene. 2003;320:185\u201390.","journal-title":"Gene"},{"key":"618_CR7","doi-asserted-by":"crossref","first-page":"S467","DOI":"10.1098\/rsif.2008.0520.focus","volume":"6","author":"M Welch","year":"2009","unstructured":"Welch M, Villalobos A, Gustafsson C, Minshull J. You\u2019re one in a googol: optimizing genes for protein expression. J R Soc Interface. 2009;6:S467\u201376.","journal-title":"J R Soc Interface"},{"key":"618_CR8","doi-asserted-by":"crossref","unstructured":"Saez NJ, Nozach H, Blemont M, Vincentelli R. High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli. J Vis Exp. 2014;(89):e51464.","DOI":"10.3791\/51464"},{"key":"618_CR9","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/978-1-62703-691-7_3","volume":"1091","author":"NJ Saez","year":"2014","unstructured":"Saez NJ, Vincentelli R. High-throughput expression screening and purification of recombinant proteins in E. coli. Methods Mol Biol. 2014;1091:33\u201353.","journal-title":"Methods Mol Biol"},{"key":"618_CR10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s40409-015-0018-7","volume":"21","author":"H Clement","year":"2015","unstructured":"Clement H, Flores V, Diego-Garcia E, Corrales-Garcia L, Villegas E, Corzo G. A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide. J Venom Anim Toxins Incl Trop Dis. 2015;21:19.","journal-title":"J Venom Anim Toxins Incl Trop Dis."},{"key":"618_CR11","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1475-2859-12-37","volume":"12","author":"H Nozach","year":"2013","unstructured":"Nozach H, Fruchart-Gaillard C, Fenaille F, Beau F, Ramos OHP, Douzi B, et al. High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli. Microb Cell Fact. 2013;12:37.","journal-title":"Microb Cell Fact"},{"key":"618_CR12","first-page":"63","volume":"5","author":"SJ Costa","year":"2014","unstructured":"Costa SJ, Almeida A, Castro A, Domingues L. Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system. Front Microbiol. 2014;5:63.","journal-title":"Front Microbiol."},{"key":"618_CR13","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1006\/abio.1994.1060","volume":"216","author":"TD Parks","year":"1994","unstructured":"Parks TD, Leuther KK, Howard ED, Johnston SA, Dougherty WG. Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase. Anal Biochem. 1994;216:413\u20137.","journal-title":"Anal Biochem"},{"key":"618_CR14","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1002\/j.1460-2075.1988.tb02942.x","volume":"7","author":"WG Dougherty","year":"1988","unstructured":"Dougherty WG, Carrington JC, Cary SM, Parks TD. Biochemical and mutational analysis of a plant virus polyprotein cleavage site. EMBO J. 1988;7:1281\u20137.","journal-title":"EMBO J"},{"key":"618_CR15","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.jmb.2006.10.085","volume":"366","author":"I Karbat","year":"2007","unstructured":"Karbat I, Turkov M, Cohen L, Kahn R, Gordon D, Gurevitz M, et al. X-ray structure and mutagenesis of the scorpion depressant toxin LqhIT2 reveals key determinants crucial for activity and anti-insect selectivity. J Mol Biol. 2007;366:586\u2013601.","journal-title":"J Mol Biol"},{"key":"618_CR16","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.jbiotec.2006.01.015","volume":"124","author":"G Wu","year":"2006","unstructured":"Wu G, Wolf JB, Ibrahim AF, Vadasz S, Gunasinghe M, Freeland SJ. Simplified gene synthesis: a one-step approach to PCR-based gene construction. J Biotechnol. 2006;124:496\u2013503.","journal-title":"J Biotechnol"},{"key":"618_CR17","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1111\/j.1574-6976.2008.00109.x","volume":"32","author":"A-S Xiong","year":"2008","unstructured":"Xiong A-S, Peng R-H, Zhuang J, Gao F, Li Y, Cheng Z-M, et al. Chemical gene synthesis: strategies, softwares, error corrections, and applications. FEMS Microbiol Rev. 2008;32:522\u201340.","journal-title":"FEMS Microbiol Rev"},{"key":"618_CR18","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1101\/gr.143000","volume":"10","author":"JL Hartley","year":"2000","unstructured":"Hartley JL, Temple GF, Brasch MA. DNA cloning using in vitro site-specific recombination. Genome Res. 2000;10:1788\u201395.","journal-title":"Genome Res"},{"key":"618_CR19","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ymeth.2011.08.010","volume":"55","author":"R Vincentelli","year":"2011","unstructured":"Vincentelli R, Cimino A, Geerlof A, Kubo A, Satou Y, Cambillau C. High-throughput protein expression screening and purification in Escherichia coli. Methods. 2011;55:65\u201372.","journal-title":"Methods"},{"key":"618_CR20","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pep.2005.01.016","volume":"41","author":"FW Studier","year":"2005","unstructured":"Studier FW. Protein production by auto-induction in high density shaking cultures. Protein Expr Purif. 2005;41:207\u201334.","journal-title":"Protein Expr Purif"},{"key":"618_CR21","doi-asserted-by":"crossref","first-page":"e63865","DOI":"10.1371\/journal.pone.0063865","volume":"8","author":"JK Klint","year":"2013","unstructured":"Klint JK, Senff S, Saez NJ, Seshadri R, Lau HY, Bende NS, et al. Production of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli. PLoS ONE. 2013;8:e63865.","journal-title":"PLoS ONE"},{"key":"618_CR22","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1124\/mol.115.098178","volume":"88","author":"FC Cardoso","year":"2015","unstructured":"Cardoso FC, Dekan Z, Rosengren KJ, Erickson A, Vetter I, Deuis JR, et al. Identification and characterization of ProTx-III [\u03bc-TRTX-Tp1a], a new voltage-gated sodium channel inhibitor from venom of the tarantula thrixopelma pruriens. Mol Pharmacol. 2015;88:291\u2013303.","journal-title":"Mol Pharmacol"},{"key":"618_CR23","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.3390\/md10071605","volume":"10","author":"R Anangi","year":"2012","unstructured":"Anangi R, Rash LD, Mobli M, King GF. Functional expression in Escherichia coli of the disulfide-rich sea anemone peptide APETx2, a potent blocker of acid-sensing ion channel 3. Mar Drugs. 2012;10:1605\u201318.","journal-title":"Mar Drugs."},{"key":"618_CR24","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1038\/ncomms5350","volume":"5","author":"NS Bende","year":"2014","unstructured":"Bende NS, Dziemborowicz S, Mobli M, Herzig V, Gilchrist J, Wagner J, et al. A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a. Nat Commun. 2014;5:4350.","journal-title":"Nat Commun."},{"key":"618_CR25","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1111\/febs.13189","volume":"282","author":"NS Bende","year":"2015","unstructured":"Bende NS, Dziemborowicz S, Herzig V, Ramanujam V, Brown GW, Bosmans F, et al. The insecticidal spider toxin SFI1 is a knottin peptide that blocks the pore of insect voltage-gated sodium channels via a large b-hairpin loop. FEBS J. 2015;282:904\u201320.","journal-title":"FEBS J"},{"key":"618_CR26","first-page":"3","volume":"6","author":"E Meng","year":"2011","unstructured":"Meng E, Cai TF, Li WY, Zhang H, Liu YB, Peng K, et al. Functional expression of spider neurotoxic peptide Huwentoxin-I in E. coli. PLoS ONE. 2011;6:3\u20138.","journal-title":"PLoS ONE"},{"key":"618_CR27","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1124\/mol.111.072207","volume":"80","author":"NJ Saez","year":"2011","unstructured":"Saez NJ, Mobli M, Bieri M, Chassagnon IR, Malde AK, Gamsjaeger R, et al. A dynamic pharmacophore drives the interaction between Psalmotoxin-1 and the putative drug target acid-sensing ion channel 1a. Mol Pharmacol. 2011;80:796\u2013808.","journal-title":"Mol Pharmacol"},{"key":"618_CR28","doi-asserted-by":"crossref","first-page":"17534","DOI":"10.1073\/pnas.1306285110","volume":"110","author":"S Yang","year":"2013","unstructured":"Yang S, Xiao Y, Kang D, Liu J, Li Y, Undheim E, et al. Discovery of a selective NaV1.7 inhibitor from centipede venom with analgesic efficacy exceeding morphine in rodent pain models. Proc Natl Acad Sci USA. 2013;110:17534\u20139.","journal-title":"Proc Natl Acad Sci USA"},{"key":"618_CR29","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.jbiotec.2005.08.006","volume":"121","author":"S Berg Van Den","year":"2006","unstructured":"Van Den Berg S, L\u00f6fdahl P\u00c5, H\u00e4rd T, Berglund H. Improved solubility of TEV protease by directed evolution. J Biotechnol. 2006;121:291\u20138.","journal-title":"J Biotechnol"},{"key":"618_CR30","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/S0006-291X(02)00574-0","volume":"294","author":"RB Kapust","year":"2002","unstructured":"Kapust RB, T\u00f6zs\u00e9r J, Copeland TD, Waugh DS. The P1\u2032 specificity of tobacco etch virus protease. Biochem Biophys Res Commun. 2002;294:949\u201355.","journal-title":"Biochem Biophys Res Commun."},{"key":"618_CR31","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.tibtech.2008.10.007","volume":"27","author":"MJ Czar","year":"2009","unstructured":"Czar MJ, Anderson JC, Bader JS, Peccoud J. Gene synthesis demystified. Trends Biotechnol. 2009;27:63\u201372.","journal-title":"Trends Biotechnol"},{"key":"618_CR32","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1128\/jb.177.6.1497-1504.1995","volume":"177","author":"H Dong","year":"1995","unstructured":"Dong H, Nilsson L, Kurland CG. Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction. J Bacteriol. 1995;177:1497\u2013504.","journal-title":"J Bacteriol"}],"container-title":["Microbial Cell Factories"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-016-0618-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,17]],"date-time":"2019-09-17T00:02:34Z","timestamp":1568678554000},"score":1,"resource":{"primary":{"URL":"http:\/\/microbialcellfactories.biomedcentral.com\/articles\/10.1186\/s12934-016-0618-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,17]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["618"],"URL":"https:\/\/doi.org\/10.1186\/s12934-016-0618-0","relation":{},"ISSN":["1475-2859"],"issn-type":[{"type":"electronic","value":"1475-2859"}],"subject":[],"published":{"date-parts":[[2017,1,17]]},"article-number":"4"}}