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MicroRNAs hold promise as noninvasive cancer biomarkers due to their inherent stability and resilience in tissues and bodily fluids. There is increasing evidence that specific microRNAs play a functional role at different steps of the metastatic cascade, behaving as signaling mediators to enable the colonization of a specific organ. Herein, we aimed to evaluate the biomarker performance of microRNAs previously reported as associated with prognosis for predicting BrC progression in liquid biopsies.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Selected microRNAs were assessed using a quantitative reverse transcription-polymerase chain reaction in a testing cohort of formalin-fixed paraffin-embedded primary (n\u2009=\u200916) and metastatic BrC tissues (n\u2009=\u200922). Then, miR-30b-5p and miR-200b-3p were assessed in a validation cohort #1 of formalin-fixed paraffin-embedded primary (n\u2009=\u200982) and metastatic BrC tissues (n\u2009=\u200993), whereas only miR-30b-5p was validated on a validation cohort #2 of liquid biopsies from BrC patients with localized (n\u2009=\u200920) and advanced (n\u2009=\u200925) disease. ROC curve was constructed to evaluate prognostic performance.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>MiR-30b-5p was differentially expressed in primary tumors and paired metastatic lesions, with bone metastases displaying significantly higher miR-30b-5p expression levels, paralleling the corresponding primary tumors. Interestingly, patients with advanced disease disclosed increased circulating miR-30b-5p expression compared to patients with localized BrC.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>MiR-30b-5p might identify BrC patients at higher risk of disease progression, thus, providing a useful clinical tool for patients\u2019 monitoring, entailing earlier and more effective treatment. Nonetheless, validation in larger multicentric cohorts is mandatory to confirm these findings.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12967-019-02193-y","type":"journal-article","created":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T11:02:39Z","timestamp":1577703759000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Overexpression of circulating MiR-30b-5p identifies advanced breast cancer"],"prefix":"10.1186","volume":"17","author":[{"given":"Helena","family":"Estev\u00e3o-Pereira","sequence":"first","affiliation":[]},{"given":"Jo\u00e3o","family":"Lobo","sequence":"additional","affiliation":[]},{"given":"Sofia","family":"Salta","sequence":"additional","affiliation":[]},{"given":"Maria","family":"Amorim","sequence":"additional","affiliation":[]},{"given":"Paula","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"Mariana","family":"Cantante","sequence":"additional","affiliation":[]},{"given":"Berta","family":"Reis","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds","family":"Antunes","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Castro","sequence":"additional","affiliation":[]},{"given":"Susana","family":"Palma de Sousa","sequence":"additional","affiliation":[]},{"given":"C\u00e9line S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[]},{"given":"Bruno M.","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Henrique","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4186-5345","authenticated-orcid":false,"given":"Carmen","family":"Jer\u00f3nimo","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,12,30]]},"reference":[{"issue":"6","key":"2193_CR1","doi-asserted-by":"publisher","first-page":"394","DOI":"10.3322\/caac.21492","volume":"68","author":"F Bray","year":"2018","unstructured":"Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. 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