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Here we report the identification and analysis of a complete genome sequence of a novel polyomavirus, <jats:italic>Raja clavata<\/jats:italic> polyomavirus (RcPyV1), from a cartilaginous fish, the thornback skate (<jats:italic>Raja clavata<\/jats:italic>). The genome sequence was determined using a metagenomics approach with an aim to provide baseline viral data in cartilaginous fish in different ecosystems. The RcPyV1 genome (4,195 nucleotides) had typical organization of polyomavirus, including early antigens (small T; Large T) encoded on one strand and late viral proteins (VP1; VP2) on the complementary strand. Maximum-likelihood phylogenetic analysis of the large T-antigen revealed that RcPyV1 clusters with a polyomavirus obtained from another cartilaginous fish, the guitarfish polyomavirus 1 (GfPyV1). These two share\u2009~\u200956% pairwise identity in LT and VP1 protein sequences. These analyses support the hypothesis that cartilaginous fishes have a specific lineage of polyomaviruses.<\/jats:p>","DOI":"10.1186\/s12985-023-02149-1","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T14:03:14Z","timestamp":1692885794000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification and characterization of a polyomavirus in the thornback skate (Raja clavata)"],"prefix":"10.1186","volume":"20","author":[{"given":"Joana","family":"Abrantes","sequence":"first","affiliation":[]},{"given":"Arvind","family":"Varsani","sequence":"additional","affiliation":[]},{"given":"Paulo","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Catarina","family":"Maia","sequence":"additional","affiliation":[]},{"given":"In\u00eas","family":"Farias","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Ver\u00edssimo","sequence":"additional","affiliation":[]},{"given":"Fabiana","family":"Neves","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,8,24]]},"reference":[{"key":"2149_CR1","doi-asserted-by":"publisher","first-page":"e1005574","DOI":"10.1371\/journal.ppat.1005574","volume":"12","author":"CB Buck","year":"2016","unstructured":"Buck CB, Van Doorslaer K, Peretti A, Geoghegan EM, Tisza MJ, An P, Katz JP, Pipas JM, McBride AA, Camus AC, McDermott AJ, Dill JA, Delwart E, Ng TF, Farkas K, Austin C, Kraberger S, Davison W, Pastrana DV, Varsani A. 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