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Although it feeds preferentially on reptiles, blood meal analyses and detection of <jats:italic>Leishmania<\/jats:italic> (<jats:italic>Leishmania<\/jats:italic>) <jats:italic>infantum<\/jats:italic> DNA in wild-caught <jats:italic>S.<\/jats:italic><jats:italic>minuta<\/jats:italic> suggest that occasional feeding may occur on mammals, including humans. Therefore, it is currently suspected as a potential vector of human pathogens.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A recently established <jats:italic>S.<\/jats:italic><jats:italic>minuta<\/jats:italic> colony was allowed to feed on three reptile species (i.e. lizard <jats:italic>Podarcis<\/jats:italic><jats:italic>siculus<\/jats:italic> and geckos <jats:italic>Tarentola<\/jats:italic><jats:italic>mauritanica<\/jats:italic> and <jats:italic>Hemidactylus<\/jats:italic><jats:italic>turcicus<\/jats:italic>) and three mammal species (i.e. mouse, rabbit and human). Sand fly mortality and fecundity were studied in blood-fed females, and the results were compared with <jats:italic>Phlebotomus<\/jats:italic><jats:italic>papatasi<\/jats:italic>, vector of <jats:italic>Leishmania<\/jats:italic> (<jats:italic>L<\/jats:italic>.) <jats:italic>major<\/jats:italic>. Blood meal volumes were measured by haemoglobinometry.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>Sergentomyia<\/jats:italic><jats:italic>minuta<\/jats:italic> fed readily on three reptile species tested, neglected the mouse and the rabbit but took a blood meal on human. However, the percentage of females engorged on human volunteer was low in cage (3%) and feeding on human blood resulted in extended defecation times, higher post-feeding mortality and lower fecundity. The average volumes of blood ingested by females fed on human and gecko were 0.97\u00a0\u00b5l and 1.02\u00a0\u00b5l, respectively. <jats:italic>Phlebotomus<\/jats:italic><jats:italic>papatasi<\/jats:italic> females readily fed on mouse, rabbit and human volunteer; a lower percentage of females (23%) took blood meal on the <jats:italic>T.<\/jats:italic><jats:italic>mauritanica<\/jats:italic> gecko; reptilian blood increased mortality post-feeding but did not affect <jats:italic>P.<\/jats:italic><jats:italic>papatasi<\/jats:italic> fecundity.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Anthropophilic behaviour of <jats:italic>S.<\/jats:italic><jats:italic>minuta<\/jats:italic> was experimentally demonstrated; although sand fly females prefer reptiles as hosts, they were attracted to the human volunteer and took a relatively high volume of blood. Their feeding times were longer than in sand fly species regularly feeding on mammals and their physiological parameters suggest that <jats:italic>S.<\/jats:italic><jats:italic>minuta<\/jats:italic> is not adapted well for digestion of mammalian blood. Nevertheless, the ability to bite humans highlights the necessity of further studies on <jats:italic>S.<\/jats:italic><jats:italic>minuta<\/jats:italic> vector competence to elucidate its potential role in circulation of <jats:italic>Leishmania<\/jats:italic> and phleboviruses pathogenic to humans.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-023-05758-5","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T18:09:46Z","timestamp":1681409386000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Experimental feeding of Sergentomyia minuta on reptiles and mammals: comparison with Phlebotomus papatasi"],"prefix":"10.1186","volume":"16","author":[{"given":"Lucie","family":"Ticha","sequence":"first","affiliation":[]},{"given":"Vera","family":"Volfova","sequence":"additional","affiliation":[]},{"given":"Jairo Alfonso","family":"Mendoza-Roldan","sequence":"additional","affiliation":[]},{"given":"Marcos Antonio","family":"Bezerra-Santos","sequence":"additional","affiliation":[]},{"given":"Carla","family":"Maia","sequence":"additional","affiliation":[]},{"given":"Jovana","family":"Sadlova","sequence":"additional","affiliation":[]},{"given":"Domenico","family":"Otranto","sequence":"additional","affiliation":[]},{"given":"Petr","family":"Volf","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,4,13]]},"reference":[{"key":"5758_CR1","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1111\/j.1365-2915.2012.01034.x","volume":"27","author":"M Maroli","year":"2013","unstructured":"Maroli M, Feliciangeli MD, Bichaud L, Charrel RN, Gradoni L. 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J Med Entomol. 2014;49:967\u201370.","journal-title":"J Med Entomol"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-023-05758-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13071-023-05758-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-023-05758-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T18:10:09Z","timestamp":1681409409000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-023-05758-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,13]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["5758"],"URL":"https:\/\/doi.org\/10.1186\/s13071-023-05758-5","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,13]]},"assertion":[{"value":"3 February 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 March 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 April 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Mice, rabbits and leopard geckos were maintained and handled in the animal facility of Charles University in Prague in accordance with institutional guidelines and Czech legislation (Act No. 246\/1992 and 359\/2012 coll. on Protection of Animals against Cruelty in present statutes at large), which complies with all relevant European Union and international guidelines for experimental animals. All experiments were approved by the Committee on the Ethics of Laboratory Experiments of the Charles University, Prague, and were performed under permissions of no. MSMT-8604\/2019-6 and MSMT-11459\/2019-4 of the Czech Ministry of Education of the Czech Republic. Investigators are certified for experimentation with animals by the Ministry of Agriculture of the Czech Republic. Protocols for reptile collection were authorized by the Ministry for Environment, Land and Sea Protection of Italy (approval number 0073267\/2019), the\u00a0<i>Societas<\/i><i>Herpetologica<\/i><i>Italica<\/i>\u00a0and the\u00a0<i>Istituto<\/i><i>Superiore<\/i><i>per<\/i><i>la<\/i><i>Protezione<\/i><i>e<\/i><i>la<\/i><i>Ricerca<\/i><i>Ambientale<\/i>\u00a0(approval number 71216). Reptile handling and maintenance were authorized by the ethical committee of the Department of Veterinary Medicine of the University of Bari, Italy (Prot. Uniba 14\/2022).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Authors declare that there are no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"126"}}