{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T20:10:01Z","timestamp":1772827801268,"version":"3.50.1"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"FCT - Funda\u00e7 \u00e3o para a Ci\u00eancia e a Tecnologia","award":["POCI-01-0145-FEDER-031402"],"award-info":[{"award-number":["POCI-01-0145-FEDER-031402"]}]},{"name":"FCT - Funda\u00e7 \u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BD\/147229\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147229\/2019"]}]},{"name":"FCT - Funda\u00e7 \u00e3o para a Ci\u00eancia e a Tecnologia","award":["CEECINST\/00091\/2018\/CP1500\/CT0011"],"award-info":[{"award-number":["CEECINST\/00091\/2018\/CP1500\/CT0011"]}]},{"name":"AO CMF","award":["AOCMFS-21-23A"],"award-info":[{"award-number":["AOCMFS-21-23A"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The vast and promising class of long non-coding RNAs (lncRNAs) has been under investigation for distinct therapeutic applications. Nevertheless, their role as molecular drivers of bone regeneration remains poorly studied. The lncRNA\n                      <jats:italic>H19<\/jats:italic>\n                      mediates osteogenic differentiation of Mesenchymal Stem\/Stromal Cells (MSCs) through the control of intracellular pathways. However, the effect of\n                      <jats:italic>H19<\/jats:italic>\n                      on the extracellular matrix (ECM) components is still largely unknown. This research study was designed to decode the\n                      <jats:italic>H19<\/jats:italic>\n                      -mediated ECM regulatory network, and to reveal how the decellularized siH19-engineered matrices influence MSC proliferation and fate. This is particularly relevant for diseases in which the ECM regulation and remodeling processes are disrupted, such as osteoporosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mass spectrometry-based quantitative proteomics analysis was used to identify ECM components, after oligonucleotides delivery to osteoporosis-derived hMSCs. Moreover, qRT-PCR, immunofluorescence and proliferation, differentiation and apoptosis assays were performed. Engineered matrices were decellularized, characterized by atomic force microscopy and repopulated with hMSC and pre-adipocytes. Clinical bone samples were characterized by histomorphometry analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our study provides an in-depth proteome-wide and matrisome-specific analysis of the ECM proteins controlled by the lncRNA\n                      <jats:italic>H19<\/jats:italic>\n                      . Using bone marrow-isolated MSC from patients with osteoporosis, we identified fibrillin-1 (FBN1), vitronectin (VTN) and collagen triple helix repeat containing 1 (CTHRC1), among others, as having different pattern levels following\n                      <jats:italic>H19<\/jats:italic>\n                      silencing. Decellularized siH19-engineered matrices are less dense and have a decreased collagen content compared with control matrices. Repopulation with na\u00efve MSCs promotes a shift towards the adipogenic lineage in detriment of the osteogenic lineage and inhibits proliferation. In pre-adipocytes, these siH19-matrices enhance lipid droplets formation. Mechanistically,\n                      <jats:italic>H19<\/jats:italic>\n                      is targeted by miR-29c, whose expression is decreased in osteoporotic bone clinical samples. Accordingly, miR-29c impacts MSC proliferation and collagen production, but does not influence ALP staining or mineralization, revealing that\n                      <jats:italic>H19<\/jats:italic>\n                      silencing and miR-29c mimics have complementary but not overlapping functions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our data suggest\n                      <jats:italic>H19<\/jats:italic>\n                      as a therapeutic target to engineer the bone ECM and to control cell behavior.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13287-023-03250-6","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T21:02:47Z","timestamp":1678222967000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Long non-coding RNA H19 regulates matrisome signature and impacts cell behavior on MSC-engineered extracellular matrices"],"prefix":"10.1186","volume":"14","author":[{"given":"Sara Reis","family":"Moura","sequence":"first","affiliation":[]},{"given":"Jaime","family":"Freitas","sequence":"additional","affiliation":[]},{"given":"Cl\u00e1udia","family":"Ribeiro-Machado","sequence":"additional","affiliation":[]},{"given":"Jorge","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Neves","sequence":"additional","affiliation":[]},{"given":"Helena","family":"Canh\u00e3o","sequence":"additional","affiliation":[]},{"given":"Ana Maria","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"M\u00e1rio Adolfo","family":"Barbosa","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2072-8587","authenticated-orcid":false,"given":"Maria In\u00eas","family":"Almeida","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,3,8]]},"reference":[{"key":"3250_CR1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-09163-0","volume-title":"Osteoporosis","author":"R Bartl","year":"2004","unstructured":"Bartl R, Frisch B. Osteoporosis. Berlin: Springer; 2004. https:\/\/doi.org\/10.1007\/978-3-662-09163-0."},{"key":"3250_CR2","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1007\/s11657-020-00871-9","volume":"16","author":"JA Kanis","year":"2021","unstructured":"Kanis JA, et al. SCOPE 2021: a new scorecard for osteoporosis in Europe. Arch Osteoporos. 2021;16:82.","journal-title":"Arch Osteoporos"},{"key":"3250_CR3","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1038\/nrrheum.2009.260","volume":"6","author":"N Harvey","year":"2010","unstructured":"Harvey N, Dennison E, Cooper C. Osteoporosis: impact on health and economics. Nat Rev Rheumatol. 2010;6:99\u2013105.","journal-title":"Nat Rev Rheumatol"},{"key":"3250_CR4","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1007\/s11657-013-0136-1","volume":"8","author":"E Hernlund","year":"2013","unstructured":"Hernlund E, et al. Osteoporosis in the European Union: medical management, epidemiology and economic burden. A report prepared in collaboration with the International Osteoporosis Foundation (IOF) and the European Federation of Pharmaceutical Industry Associations (EFPIA). Arch Osteoporos. 2013;8:136.","journal-title":"Arch Osteoporos"},{"key":"3250_CR5","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1038\/nrrheum.2009.139","volume":"5","author":"JA Kanis","year":"2009","unstructured":"Kanis JA, McCloskey EV, Johansson H, Oden A. Approaches to the targeting of treatment for osteoporosis. Nat Rev Rheumatol. 2009;5:425\u201331.","journal-title":"Nat Rev Rheumatol"},{"key":"3250_CR6","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1038\/s41413-019-0048-9","volume":"7","author":"AM Silva","year":"2019","unstructured":"Silva AM, et al. Long noncoding RNAs: a missing link in osteoporosis. Bone Res. 2019;7:10.","journal-title":"Bone Res"},{"key":"3250_CR7","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1503\/cmaj.070234","volume":"177","author":"A Cranney","year":"2007","unstructured":"Cranney A, Jamal SA, Tsang JF, Josse RG, Leslie WD. Low bone mineral density and fracture burden in postmenopausal women. CMAJ. 2007;177:575\u201380.","journal-title":"CMAJ"},{"key":"3250_CR8","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1007\/s00198-003-1489-x","volume":"14","author":"ML Bouxsein","year":"2003","unstructured":"Bouxsein ML. Bone quality: where do we go from here? Osteoporos Int. 2003;14:118\u201327.","journal-title":"Osteoporos Int"},{"key":"3250_CR9","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1093\/abbs\/gmaa025","volume":"52","author":"D Zhang","year":"2020","unstructured":"Zhang D, et al. Osteoporosis-decreased extracellular matrix stiffness impairs connexin 43-mediated gap junction intercellular communication in osteocytes. Acta Biochim Biophys Sin. 2020;52:517\u201326.","journal-title":"Acta Biochim Biophys Sin"},{"key":"3250_CR10","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1007\/s00223-018-0508-z","volume":"104","author":"R Ozasa","year":"2019","unstructured":"Ozasa R, et al. Osteoporosis changes collagen\/apatite orientation and young\u2019s modulus in vertebral cortical bone of rat. Calcif Tissue Int. 2019;104:449\u201360.","journal-title":"Calcif Tissue Int"},{"key":"3250_CR11","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1016\/j.bone.2012.08.129","volume":"51","author":"AM Rodrigues","year":"2012","unstructured":"Rodrigues AM, et al. Low osteocalcin\/collagen type I bone gene expression ratio is associated with hip fragility fractures. Bone. 2012;51:981\u20139.","journal-title":"Bone"},{"key":"3250_CR12","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/s12920-015-0149-2","volume":"8","author":"L De-Ugarte","year":"2015","unstructured":"De-Ugarte L, et al. MiRNA profiling of whole trabecular bone: identification of osteoporosis-related changes in MiRNAs in human hip bones. BMC Med Genomics. 2015;8:75.","journal-title":"BMC Med Genomics"},{"key":"3250_CR13","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.bone.2008.08.120","volume":"44","author":"B Hopwood","year":"2009","unstructured":"Hopwood B, Tsykin A, Findlay DM, Fazzalari NL. Gene expression profile of the bone microenvironment in human fragility fracture bone. Bone. 2009;44:87\u2013101.","journal-title":"Bone"},{"key":"3250_CR14","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/S8756-3282(99)00279-3","volume":"26","author":"H Kafantari","year":"2000","unstructured":"Kafantari H, Kounadi E, Fatouros M, Milonakis M, Tzaphlidou M. Structural alterations in rat skin and bone collagen fibrils induced by ovariectomy. Bone. 2000;26:349\u201353.","journal-title":"Bone"},{"key":"3250_CR15","doi-asserted-by":"publisher","first-page":"20180793","DOI":"10.1098\/rsif.2018.0793","volume":"16","author":"R M\u00fcller","year":"2019","unstructured":"M\u00fcller R, et al. Analysis of microscopic bone properties in an osteoporotic sheep model: a combined biomechanics, FE and ToF-SIMS study. J R Soc Interface. 2019;16:20180793.","journal-title":"J R Soc Interface"},{"key":"3250_CR16","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/s11914-012-0101-8","volume":"10","author":"JS Nyman","year":"2012","unstructured":"Nyman JS, Makowski AJ. The contribution of the extracellular matrix to the fracture resistance of bone. Curr Osteoporos Rep. 2012;10:169\u201377.","journal-title":"Curr Osteoporos Rep"},{"key":"3250_CR17","doi-asserted-by":"publisher","first-page":"C111","DOI":"10.1152\/ajpcell.00120.2019","volume":"318","author":"PH Schlesinger","year":"2020","unstructured":"Schlesinger PH, et al. Cellular and extracellular matrix of bone, with principles of synthesis and dependency of mineral deposition on cell membrane transport. Am J Physiol Cell Physiol. 2020;318:C111\u201324.","journal-title":"Am J Physiol Cell Physiol"},{"key":"3250_CR18","doi-asserted-by":"crossref","unstructured":"Al-Bari AA, Al Mamun, A. Current advances in regulation of bone homeostasis. FASEB Bioadv 2, 668\u2013679 (2020).","DOI":"10.1096\/fba.2020-00058"},{"key":"3250_CR19","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.jsbmb.2013.09.008","volume":"142","author":"SH Tella","year":"2014","unstructured":"Tella SH, Gallagher JC. Prevention and treatment of postmenopausal osteoporosis. J Steroid Biochem Mol Biol. 2014;142:155\u201370.","journal-title":"J Steroid Biochem Mol Biol"},{"key":"3250_CR20","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1007\/s11914-013-0135-6","volume":"11","author":"M Bethel","year":"2013","unstructured":"Bethel M, Chitteti BR, Srour EF, Kacena MA. The changing balance between osteoblastogenesis and adipogenesis in aging and its impact on hematopoiesis. Curr Osteoporos Rep. 2013;11:99\u2013106.","journal-title":"Curr Osteoporos Rep"},{"key":"3250_CR21","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1038\/cdd.2015.168","volume":"23","author":"Q Chen","year":"2016","unstructured":"Chen Q, et al. Fate decision of mesenchymal stem cells: adipocytes or osteoblasts? Cell Death Differ. 2016;23:1128\u201339.","journal-title":"Cell Death Differ"},{"key":"3250_CR22","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1186\/s13287-019-1498-0","volume":"10","author":"L Han","year":"2019","unstructured":"Han L, et al. The shift in the balance between osteoblastogenesis and adipogenesis of mesenchymal stem cells mediated by glucocorticoid receptor. Stem Cell Res Ther. 2019;10:377.","journal-title":"Stem Cell Res Ther"},{"key":"3250_CR23","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1159\/000489053","volume":"1","author":"J Li","year":"2018","unstructured":"Li J, Zuo B, Zhang L, Dai L, Zhang X. Osteoblast versus adipocyte: bone marrow microenvironment-guided epigenetic control. Case Rep Orthop Res. 2018;1:2\u201318.","journal-title":"Case Rep Orthop Res"},{"key":"3250_CR24","doi-asserted-by":"publisher","first-page":"746","DOI":"10.1016\/j.bone.2015.05.026","volume":"81","author":"MQ Hassan","year":"2015","unstructured":"Hassan MQ, Tye CE, Stein GS, Lian JB. Non-coding RNAs: epigenetic regulators of bone development and homeostasis. Bone. 2015;81:746\u201356.","journal-title":"Bone"},{"key":"3250_CR25","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.584118","volume":"11","author":"S Patil","year":"2020","unstructured":"Patil S, Dang K, Zhao X, Gao Y, Qian A. Role of LncRNAs and CircRNAs in bone metabolism and osteoporosis. Front Genet. 2020;11: 584118.","journal-title":"Front Genet"},{"key":"3250_CR26","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1093\/nsr\/nwu008","volume":"1","author":"X-D Fu","year":"2014","unstructured":"Fu X-D. Non-coding RNA: a new frontier in regulatory biology. Natl Sci Rev. 2014;1:190\u2013204.","journal-title":"Natl Sci Rev"},{"key":"3250_CR27","doi-asserted-by":"publisher","first-page":"3909","DOI":"10.3390\/ijms22083909","volume":"22","author":"C Aurilia","year":"2021","unstructured":"Aurilia C, et al. The involvement of long non-coding RNAs in bone. Int J Mol Sci. 2021;22:3909.","journal-title":"Int J Mol Sci"},{"key":"3250_CR28","doi-asserted-by":"publisher","first-page":"20121","DOI":"10.1038\/srep20121","volume":"6","author":"W-C Liang","year":"2016","unstructured":"Liang W-C, et al. H19 activates Wnt signaling and promotes osteoblast differentiation by functioning as a competing endogenous RNA. Sci Rep. 2016;6:20121.","journal-title":"Sci Rep"},{"key":"3250_CR29","doi-asserted-by":"publisher","first-page":"4944","DOI":"10.1111\/jcmm.15040","volume":"24","author":"G Li","year":"2020","unstructured":"Li G, et al. Long non-coding RNA-H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA-149\/SDF-1 axis. J Cell Mol Med. 2020;24:4944\u201355.","journal-title":"J Cell Mol Med"},{"key":"3250_CR30","doi-asserted-by":"publisher","first-page":"3481","DOI":"10.1002\/stem.2225","volume":"33","author":"Y Huang","year":"2015","unstructured":"Huang Y, Zheng Y, Jia L, Li W. Long noncoding RNA H19 promotes osteoblast differentiation via TGF-\u03b21\/Smad3\/HDAC signaling pathway by deriving miR-675. Stem Cells. 2015;33:3481\u201392.","journal-title":"Stem Cells"},{"key":"3250_CR31","doi-asserted-by":"publisher","first-page":"7715","DOI":"10.7150\/thno.58410","volume":"11","author":"J Behera","year":"2021","unstructured":"Behera J, Kumar A, Voor MJ, Tyagi N. Exosomal lncRNA-H19 promotes osteogenesis and angiogenesis through mediating Angpt1\/Tie2-NO signaling in CBS-heterozygous mice. Theranostics. 2021;11:7715\u201334.","journal-title":"Theranostics"},{"key":"3250_CR32","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.bone.2017.12.013","volume":"108","author":"J Wu","year":"2018","unstructured":"Wu J, et al. Long non-coding RNA H19 mediates mechanical tension-induced osteogenesis of bone marrow mesenchymal stem cells via FAK by sponging miR-138. Bone. 2018;108:62\u201370.","journal-title":"Bone"},{"key":"3250_CR33","doi-asserted-by":"publisher","first-page":"7435","DOI":"10.1002\/jcp.26589","volume":"233","author":"Y Wang","year":"2018","unstructured":"Wang Y, et al. Long noncoding RNA H19 mediates LCoR to impact the osteogenic and adipogenic differentiation of mBMSCs in mice through sponging miR-188. J Cell Physiol. 2018;233:7435\u201346.","journal-title":"J Cell Physiol"},{"key":"3250_CR34","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.bone.2018.03.017","volume":"111","author":"B Li","year":"2018","unstructured":"Li B, et al. Overexpression of DNMT1 leads to hypermethylation of H19 promoter and inhibition of Erk signaling pathway in disuse osteoporosis. Bone. 2018;111:82\u201391.","journal-title":"Bone"},{"key":"3250_CR35","doi-asserted-by":"publisher","first-page":"7","DOI":"10.18632\/oncotarget.6589","volume":"7","author":"MI Almeida","year":"2016","unstructured":"Almeida MI, et al. miR-195 in human primary mesenchymal stromal\/stem cells regulates proliferation, osteogenesis and paracrine effect on angiogenesis. Oncotarget. 2016;7:7\u201322.","journal-title":"Oncotarget"},{"key":"3250_CR36","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","volume":"55","author":"SA Bustin","year":"2009","unstructured":"Bustin SA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55:611\u201322.","journal-title":"Clin Chem"},{"key":"3250_CR37","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2020.115303","volume":"134","author":"SR Moura","year":"2020","unstructured":"Moura SR, et al. miR-99a in bone homeostasis: regulating osteogenic lineage commitment and osteoclast differentiation. Bone. 2020;134: 115303.","journal-title":"Bone"},{"key":"3250_CR38","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1111\/j.1699-0463.1988.tb05320.x","volume":"96","author":"HJG Gundersen","year":"1988","unstructured":"Gundersen HJG, et al. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS. 1988;96:379\u201394.","journal-title":"APMIS"},{"key":"3250_CR39","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1111\/j.1365-2818.1987.tb02837.x","volume":"147","author":"HJG Gundersen","year":"1987","unstructured":"Gundersen HJG, Jensen EB. The efficiency of systematic sampling in stereology and its prediction*. J Microsc. 1987;147:229\u201363.","journal-title":"J Microsc"},{"key":"3250_CR40","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/s12881-020-0948-y","volume":"21","author":"G Xiaoling","year":"2020","unstructured":"Xiaoling G, Shuaibin L, Kailu L. MicroRNA-19b-3p promotes cell proliferation and osteogenic differentiation of BMSCs by interacting with lncRNA H19. BMC Med Genet. 2020;21:11.","journal-title":"BMC Med Genet"},{"key":"3250_CR41","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2021.111171","volume":"527","author":"T Li","year":"2021","unstructured":"Li T, Jiang H, Li Y, Zhao X, Ding H. Estrogen promotes lncRNA H19 expression to regulate osteogenic differentiation of BMSCs and reduce osteoporosis via miR-532-3p\/SIRT1 axis. Mol Cell Endocrinol. 2021;527: 111171.","journal-title":"Mol Cell Endocrinol"},{"key":"3250_CR42","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1111\/os.12321","volume":"9","author":"B Li","year":"2017","unstructured":"Li B, et al. LncRNA-H19 modulates Wnt\/\u03b2-catenin signaling by targeting Dkk4 in Hindlimb unloaded rat. Orthop Surg. 2017;9:319\u201327.","journal-title":"Orthop Surg"},{"key":"3250_CR43","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.matbio.2015.09.004","volume":"52\u201354","author":"S Smaldone","year":"2016","unstructured":"Smaldone S, Ramirez F. Fibrillin microfibrils in bone physiology. Matrix Biol. 2016;52\u201354:191\u20137.","journal-title":"Matrix Biol"},{"key":"3250_CR44","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1083\/jcb.201003089","volume":"190","author":"H Nistala","year":"2010","unstructured":"Nistala H, et al. Fibrillin-1 and -2 differentially modulate endogenous TGF-\u03b2 and BMP bioavailability during bone formation. J Cell Biol. 2010;190:1107\u201321.","journal-title":"J Cell Biol"},{"key":"3250_CR45","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1007\/s00441-011-1167-9","volume":"344","author":"S Smaldone","year":"2011","unstructured":"Smaldone S, Carta L, Ramirez F. Establishment of fibrillin-deficient osteoprogenitor cell lines identifies molecular abnormalities associated with extracellular matrix perturbation of osteogenic differentiation. Cell Tissue Res. 2011;344:511\u20137.","journal-title":"Cell Tissue Res"},{"key":"3250_CR46","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1002\/dvg.22022","volume":"50","author":"JR Cook","year":"2012","unstructured":"Cook JR, et al. Generation of Fbn1 conditional null mice implicates the extracellular microfibrils in osteoprogenitor recruitment. Genesis. 2012;50:635\u201341.","journal-title":"Genesis"},{"key":"3250_CR47","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1177\/002215549704500805","volume":"45","author":"DR Keene","year":"1997","unstructured":"Keene DR, et al. Fibrillin-1 in human cartilage: developmental expression and formation of special banded fibers. J Histochem Cytochem. 1997;45:1069\u201382.","journal-title":"J Histochem Cytochem"},{"key":"3250_CR48","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1002\/iub.1203","volume":"65","author":"DI Leavesley","year":"2013","unstructured":"Leavesley DI, et al. Vitronectin\u2013master controller or micromanager? IUBMB Life. 2013;65:807\u201318.","journal-title":"IUBMB Life"},{"key":"3250_CR49","doi-asserted-by":"publisher","first-page":"6590","DOI":"10.1167\/iovs.14-15054","volume":"55","author":"S Chow","year":"2014","unstructured":"Chow S, Di Girolamo N. Vitronectin: a migration and wound healing factor for human corneal epithelial cells. Invest Ophthalmol Vis Sci. 2014;55:6590\u2013600.","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"3250_CR50","doi-asserted-by":"publisher","first-page":"3507","DOI":"10.1242\/jcs.114.19.3507","volume":"114","author":"AK Scaffidi","year":"2001","unstructured":"Scaffidi AK, Moodley YP, Weichselbaum M, Thompson PJ, Knight DA. Regulation of human lung fibroblast phenotype and function by vitronectin and vitronectin integrins. J Cell Sci. 2001;114:3507\u201316.","journal-title":"J Cell Sci"},{"key":"3250_CR51","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1038\/cdd.2017.153","volume":"25","author":"S-K Min","year":"2018","unstructured":"Min S-K, Kang HK, Jung SY, Jang DH, Min B-M. A vitronectin-derived peptide reverses ovariectomy-induced bone loss via regulation of osteoblast and osteoclast differentiation. Cell Death Differ. 2018;25:268\u201381.","journal-title":"Cell Death Differ"},{"key":"3250_CR52","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1016\/S1357-2725(99)00005-9","volume":"31","author":"I Schvartz","year":"1999","unstructured":"Schvartz I, Seger D, Shaltiel S. Vitronectin. Int J Biochem Cell Biol. 1999;31:539\u201344.","journal-title":"Int J Biochem Cell Biol"},{"key":"3250_CR53","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1186\/s13287-020-01682-y","volume":"11","author":"U Goyal","year":"2020","unstructured":"Goyal U, Ta M. A novel role of vitronectin in promoting survival of mesenchymal stem cells under serum deprivation stress. Stem Cell Res Ther. 2020;11:181.","journal-title":"Stem Cell Res Ther"},{"key":"3250_CR54","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1155\/S1110724304306017","volume":"2004","author":"RM Salasznyk","year":"2004","unstructured":"Salasznyk RM, Williams WA, Boskey A, Batorsky A, Plopper GE. Adhesion to vitronectin and collagen I promotes osteogenic differentiation of human mesenchymal stem cells. J Biomed Biotechnol. 2004;2004:24\u201334.","journal-title":"J Biomed Biotechnol"},{"key":"3250_CR55","doi-asserted-by":"publisher","first-page":"2426","DOI":"10.3390\/ijms22052426","volume":"22","author":"A Myngbay","year":"2021","unstructured":"Myngbay A, Manarbek L, Ludbrook S, Kunz J. The role of collagen triple helix repeat-containing 1 protein (CTHRC1) in rheumatoid arthritis. Int J Mol Sci. 2021;22:2426.","journal-title":"Int J Mol Sci"},{"key":"3250_CR56","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1016\/j.modgep.2006.03.008","volume":"6","author":"T Durmus","year":"2006","unstructured":"Durmus T, et al. Expression analysis of the novel gene collagen triple helix repeat containing-1 (Cthrc1). Gene Expr Patterns. 2006;6:935\u201340.","journal-title":"Gene Expr Patterns"},{"key":"3250_CR57","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003174","volume":"3","author":"H Kimura","year":"2008","unstructured":"Kimura H, et al. Cthrc1 is a positive regulator of osteoblastic bone formation. PLoS ONE. 2008;3: e3174.","journal-title":"PLoS ONE"},{"key":"3250_CR58","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1007\/s10735-017-9729-0","volume":"48","author":"C Wang","year":"2017","unstructured":"Wang C, et al. CTHRC1 promotes osteogenic differentiation of periodontal ligament stem cells by regulating TAZ. J Mol Hist. 2017;48:311\u20139.","journal-title":"J Mol Hist"},{"key":"3250_CR59","doi-asserted-by":"publisher","first-page":"5874","DOI":"10.1073\/pnas.0801130105","volume":"105","author":"S Sengupta","year":"2008","unstructured":"Sengupta S, et al. MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins. Proc Natl Acad Sci U S A. 2008;105:5874\u20138.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3250_CR60","doi-asserted-by":"publisher","first-page":"13027","DOI":"10.1073\/pnas.0805038105","volume":"105","author":"E van Rooij","year":"2008","unstructured":"van Rooij E, et al. Dysregulation of microRNAs after myocardial infarction reveals a role of miR-29 in cardiac fibrosis. Proc Natl Acad Sci U S A. 2008;105:13027\u201332.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3250_CR61","doi-asserted-by":"publisher","first-page":"5258","DOI":"10.3390\/cancers13215258","volume":"13","author":"SR Moura","year":"2021","unstructured":"Moura SR, et al. Circulating microRNAs correlate with multiple myeloma and skeletal osteolytic lesions. Cancers. 2021;13:5258.","journal-title":"Cancers"},{"key":"3250_CR62","doi-asserted-by":"publisher","first-page":"D543","DOI":"10.1093\/nar\/gkab1038","volume":"50","author":"Y Perez-Riverol","year":"2022","unstructured":"Perez-Riverol Y, et al. The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences. Nucleic Acids Res. 2022;50:D543\u201352.","journal-title":"Nucleic Acids Res"}],"container-title":["Stem Cell Research &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-023-03250-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13287-023-03250-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-023-03250-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T08:43:59Z","timestamp":1702025039000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-023-03250-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,8]]},"references-count":62,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["3250"],"URL":"https:\/\/doi.org\/10.1186\/s13287-023-03250-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-1862429\/v1","asserted-by":"object"}]},"ISSN":["1757-6512"],"issn-type":[{"value":"1757-6512","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,8]]},"assertion":[{"value":"18 July 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 December 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 March 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethics Committee of the Centro Hospitalar Universit\u00e1rio S\u00e3o Jo\u00e3o; Approval Reference number 70\/18.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"37"}}