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In pigs, <jats:italic>H. suis<\/jats:italic> can induce gastritis and may play a role in gastric ulcer disease, possibly in association with <jats:italic>Fusobacterium gastrosuis<\/jats:italic>. In the present study, gastric samples from 71 slaughtered pigs and 14 hunted free range wild boars were tested for the presence of DNA of <jats:italic>F. gastrosuis<\/jats:italic> and gastric <jats:italic>Helicobacter<\/jats:italic> species associated with pigs, dogs cats and humans, using species-specific PCR assays, followed by sequencing of the amplicon. These gastric samples were also histopathologically evaluated. Almost all the pigs presented gastritis (95.8%). <jats:italic>Helicobacter<\/jats:italic> spp<jats:italic>.<\/jats:italic> were detected in 78.9% and <jats:italic>F. gastrosuis<\/jats:italic> in 35.2% of the animals. <jats:italic>H. suis<\/jats:italic> was the most frequently identified <jats:italic>Helicobacter<\/jats:italic> species (57.7% of the animals), followed by a <jats:italic>H. pylori<\/jats:italic>-like species (50.7%) and less often <jats:italic>H. salomonis<\/jats:italic> and <jats:italic>H. felis<\/jats:italic> (each in 2.8% of the animals). <jats:italic>H. suis<\/jats:italic> was most often detected in the glandular (distal) part of the stomach (<jats:italic>pars oesophagea<\/jats:italic> 9.9%, oxyntic mucosa 35.2%, antral mucosa 40.8%), while the <jats:italic>H. pylori<\/jats:italic>-like species was mainly found in the non-glandular (proximal) part of the stomach (<jats:italic>pars oesophagea<\/jats:italic> 39.4%, oxyntic mucosa 14.1%, antral mucosa 4.2%). The great majority of wild boars were also affected with gastritis (71.4%) and <jats:italic>Helicobacter<\/jats:italic> spp<jats:italic>.<\/jats:italic> and <jats:italic>F. gastrosuis<\/jats:italic> were detected in 64.3% and 42.9% of the animals, respectively. <jats:italic>H. bizzozeronii<\/jats:italic> and <jats:italic>H. salomonis<\/jats:italic> were the most frequently detected <jats:italic>Helicobacter<\/jats:italic> species, while a <jats:italic>H. pylori-<\/jats:italic>like species and <jats:italic>H. suis<\/jats:italic> were only occasionally identified. These findings suggest that these microorganisms can colonize the stomach of both porcine species and may be associated with gastric pathology. This should, however, be confirmed through bacterial isolation. This is the first description of the presence of <jats:italic>F. gastrosuis<\/jats:italic> DNA in the stomach of wild boars and a <jats:italic>H. pylori-<\/jats:italic>like species in the <jats:italic>pars oesophagea<\/jats:italic> of the porcine stomach.<\/jats:p>","DOI":"10.1186\/s13567-022-01101-5","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T18:02:35Z","timestamp":1665252155000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations"],"prefix":"10.1186","volume":"53","author":[{"given":"Francisco","family":"Cortez Nunes","sequence":"first","affiliation":[]},{"given":"Teresa","family":"Letra Mateus","sequence":"additional","affiliation":[]},{"given":"Emily","family":"Taillieu","sequence":"additional","affiliation":[]},{"given":"S\u00edlvia","family":"Teixeira","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Carolino","sequence":"additional","affiliation":[]},{"given":"Alexandra","family":"Rema","sequence":"additional","affiliation":[]},{"given":"Sofie","family":"De Bruyckere","sequence":"additional","affiliation":[]},{"given":"F\u00e1tima","family":"G\u00e4rtner","sequence":"additional","affiliation":[]},{"given":"Freddy","family":"Haesebrouck","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2305-1631","authenticated-orcid":false,"given":"Irina","family":"Amorim","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2022,10,8]]},"reference":[{"key":"1101_CR1","doi-asserted-by":"publisher","first-page":"3311","DOI":"10.1128\/JCM.39.9.3311-3315.2001","volume":"39","author":"YK Choi","year":"2001","unstructured":"Choi YK, Han JH, Joo HS (2001) Identification of novel Helicobacter species in pig stomachs by PCR and partial sequencing. 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