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The rise of antibiotic selective pressure together with ongoing limitations on their use, demands new strategies to tackle this pathology. The pertinence of using bacteriophages as an alternative is being explored, and in this work, the efficacy of phage vB_EcoM_FJ1 (FJ1) in reducing the load of ETEC EC43-Ph (serotype O9:H9 expressing the enterotoxin STa and two adhesins F5 and F41) was assessed. Foreseeing the oral application on piglets, FJ1 was encapsulated on calcium carbonate and alginate microparticles, thus preventing phage release under adverse conditions of the simulated gastric fluid (pH 3.0) and allowing phage availability in simulated intestinal fluid (pH 6.5). A single dose of encapsulated FJ1, provided to IPEC-1 cultured cells (from intestinal epithelium of piglets) previously infected by EC43, provided bacterial reductions of about 99.9% after 6\u00a0h. Although bacteriophage-insensitive mutants (BIMs) have emerged from treatment, the consequent fitness costs associated with this new phenotype were demonstrated, comparatively to the originating strain. The higher competence of the pig complement system to decrease BIMs\u2019 viability, the lower level of colonization of IPEC-1 cells observed with these mutants, and the increased survival rates and health index recorded in infected <jats:italic>Galleria mellonella<\/jats:italic> larvae supported this observation. Most of all, FJ1 established a proof-of-concept of the efficiency of phages to fight against ETEC in piglet intestinal cells.<\/jats:p>","DOI":"10.1186\/s13567-023-01157-x","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T14:09:02Z","timestamp":1679494142000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of phage vB_EcoM_FJ1 on the reduction of ETEC O9:H9 infection in a neonatal pig cell line"],"prefix":"10.1186","volume":"54","author":[{"given":"Alice","family":"Ferreira","sequence":"first","affiliation":[]},{"given":"Daniela","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Carina","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Maria Elisa","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"S\u00f3nia","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Castro","sequence":"additional","affiliation":[]},{"given":"Dalila","family":"Mil-Homens","sequence":"additional","affiliation":[]},{"given":"Isidro","family":"Garc\u00eda-Meni\u00f1o","sequence":"additional","affiliation":[]},{"given":"Azucena","family":"Mora","sequence":"additional","affiliation":[]},{"given":"Mariana","family":"Henriques","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6477-5345","authenticated-orcid":false,"given":"Ana","family":"Oliveira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"key":"1157_CR1","doi-asserted-by":"publisher","first-page":"885253","DOI":"10.3389\/fimmu.2022.885253","volume":"13","author":"K Kim","year":"2022","unstructured":"Kim K, Song M, Liu Y, Ji P (2022) Enterotoxigenic Escherichia coli infection of weaned pigs: intestinal challenges and nutritional intervention to enhance disease resistance. 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ALS declares that it has no competing interest and allows the publication of the data described herein related to the phage belonging to the phage library of the company.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"26"}}