{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:41:40Z","timestamp":1790458900661,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2005,4,21]],"date-time":"2005-04-21T00:00:00Z","timestamp":1114041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004008","name":"Istituto Superiore di Sanit\u00e0","doi-asserted-by":"publisher","award":["#50F\/G"],"award-info":[{"award-number":["#50F\/G"]}],"id":[{"id":"10.13039\/501100004008","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004008","name":"Istituto Superiore di Sanit\u00e0","doi-asserted-by":"publisher","award":["5303"],"award-info":[{"award-number":["5303"]}],"id":[{"id":"10.13039\/501100004008","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,4,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The only available vaccine against tuberculosis is Mycobacterium bovis Bacillus Calmette Gu\u00e9rin (BCG), although its efficacy in preventing pulmonary tuberculosis is controversial. Early interactions between dendritic cells (DC) and BCG or Mycobacterium tuberculosis (Mtb) are thought to be critical for mounting a protective antimycobacterial immune response. Recent studies have shown that BCG and Mtb target the DC-specific C-type lectin intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) to infect DC and inhibit their immunostimulatory function. This would occur through the interaction of the mycobacterial mannosylated lipoarabinomannan to DC-SIGN, which would prevent DC maturation and induce the immunosuppressive cytokine interleukin (IL)-10 synthesis. Here, we confirm that DC-SIGN is expressed in DC derived from monocytes cultured in granulocyte macrophage-colony stimulating factor (GM-CSF) and IL-4 and show that it is not expressed in DC derived from monocytes cultured in GM-CSF and interferon-\u03b1 (IFN-\u03b1). We also demonstrate that DC-SIGN\u2013 DC cultured in GM-CSF and IFN-\u03b1 are able to phagocytose BCG and to undergo a maturation program as well as DC-SIGN+ DC cultured in IL-4 and GM-CSF. We also show that BCG causes the impairment of IL-12 and the induction of IL-10 secretion by DC, irrespective of DC-SIGN expression. Finally, we demonstrate that the capacity to stimulate a mixed leukocyte reaction of na\u00efve T lymphocytes is not altered by the treatment of both DC populations with BCG. These data suggest that DC-SIGN cannot be considered as the unique DC receptor for BCG internalization, and it is more interesting that the mycobacteria-induced immunosuppression cannot be attributed to the engagement of a single receptor.<\/jats:p>","DOI":"10.1189\/jlb.0105037","type":"journal-article","created":{"date-parts":[[2005,4,22]],"date-time":"2005-04-22T00:25:42Z","timestamp":1114129542000},"page":"106-113","source":"Crossref","is-referenced-by-count":54,"title":["<i>Mycobacterium bovis<\/i>Bacillus Calmette-Gu\u00e9rin infects DC-SIGN\u2013 dendritic cell and causes the inhibition of IL-12 and the enhancement of IL-10 production"],"prefix":"10.1093","volume":"78","author":[{"given":"Maria Cristina","family":"Gagliardi","sequence":"first","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaela","family":"Teloni","sequence":"additional","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Giannoni","sequence":"additional","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Pardini","sequence":"additional","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Sargentini","sequence":"additional","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Brunori","sequence":"additional","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanfranco","family":"Fattorini","sequence":"additional","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Nisini","sequence":"additional","affiliation":[{"name":"Dipartimento Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanit\u00e0 , Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,4,21]]},"reference":[{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1146\/annurev.immunol.19.1.93","article-title":"Immunology of tuberculosis","volume":"19","author":"Flynn","year":"2001","journal-title":"Annu. Rev. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/35095558","article-title":"How can immunology contribute to the control of tuberculosis?","volume":"1","author":"Kaufmann","year":"2001","journal-title":"Nat. Rev. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1378\/chest.117.5.1455","article-title":"Phthisiology at the dawn of the new century","volume":"117","author":"Lauzardo","year":"2000","journal-title":"Chest"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1084\/jem.163.2.436","article-title":"Dendritic cells with antigen-presenting capability reside in airway epithelium, lung parenchyma, and visceral pleura","volume":"163","author":"Sertl","year":"1986","journal-title":"J. Exp. Med."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1126\/science.290.5489.92","article-title":"Dynamics of T lymphocyte responses: intermediates, effectors, and memory cells","volume":"290","author":"Lanzavecchia","year":"2000","journal-title":"Science"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0092-8674(01)00455-X","article-title":"Regulation of T cell immunity by dendritic cells","volume":"106","author":"Lanzavecchia","year":"2001","journal-title":"Cell"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1084\/jem.20021964","article-title":"A dangerous liaison between two major killers: Mycobacterium tuberculosis and HIV target dendritic cells through DC-SIGN","volume":"197","author":"Kaufmann","year":"2003","journal-title":"J. Exp. Med."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1126\/science.1062060","article-title":"Sensing pathogens and tuning immune responses","volume":"293","author":"Pulendran","year":"2001","journal-title":"Science"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1164\/ajrccm.157.3.9708002","article-title":"The host immune response to tuberculosis","volume":"157","author":"Schluger","year":"1998","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"3848","DOI":"10.1016\/j.vaccine.2004.07.009","article-title":"Bacillus Calmette-Guerin shares with virulent Mycobacterium tuberculosis the capacity to subvert monocyte differentiation into dendritic cell: implication for its efficacy as a vaccine preventing tuberculosis","volume":"22","author":"Gagliardi","year":"2004","journal-title":"Vaccine"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"4385","DOI":"10.1128\/IAI.72.8.4385-4392.2004","article-title":"Mycobacterium tuberculosis diverts \u03b1 interferon-induced monocyte differentiation from dendritic cells into immunoprivileged macrophage-like host cells","volume":"72","author":"Mariotti","year":"2004","journal-title":"Infect. Immun."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1002\/1521-4141(200211)32:11<3050::AID-IMMU3050>3.0.CO;2-K","article-title":"Mycobacterium tuberculosis subverts the differentiation of human monocyte into dendritic cell","volume":"32","author":"Mariotti","year":"2002","journal-title":"Eur. J. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1084\/jem.20021229","article-title":"Mycobacteria target DC-SIGN to suppress dendritic cell function","volume":"197","author":"Geijtenbeek","year":"2003","journal-title":"J. Exp. Med."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1084\/jem.20021468","article-title":"DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells","volume":"197","author":"Tailleux","year":"2003","journal-title":"J. Exp. 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Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/S0952-7915(97)80153-7","article-title":"Origin, maturation and antigen presenting function of dendritic cells","volume":"9","author":"Cella","year":"1997","journal-title":"Curr. Opin. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1084\/jem.179.4.1109","article-title":"Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte\/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor \u03b1","volume":"179","author":"Sallusto","year":"1994","journal-title":"J. Exp. 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Biol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.abb.2004.04.019","article-title":"IL-4 modulates transcriptional control of the mannose receptor in mouse FSDC dendritic cells","volume":"428","author":"Egan","year":"2004","journal-title":"Arch. Biochem. Biophys."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/S0092-8674(00)80694-7","article-title":"DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells","volume":"100","author":"Geijtenbeek","year":"2000","journal-title":"Cell"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"6841","DOI":"10.1128\/JVI.76.13.6841-6844.2002","article-title":"C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans","volume":"76","author":"Alvarez","year":"2002","journal-title":"J. Virol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1517\/14728222.6.4.423","article-title":"The role of DC-SIGN and DC-SIGNR in HIV and Ebola virus infection: can potential therapeutics block virus transmission and dissemination?","volume":"6","author":"Baribaud","year":"2002","journal-title":"Expert Opin. Ther. Targets"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/S1074-7613(02)00447-8","article-title":"Human cytomegalovirus binding to DC-SIGN is required for dendritic cell infection and target cell trans-infection","volume":"17","author":"Halary","year":"2002","journal-title":"Immunity"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"4498","DOI":"10.1073\/pnas.0831128100","article-title":"L-SIGN (CD 209L) is a liver-specific capture receptor for hepatitis C virus","volume":"100","author":"Gardner","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.4049\/jimmunol.172.2.1186","article-title":"The dendritic cell receptor DC-SIGN discriminates among species and life cycle forms of Leishmania","volume":"172","author":"Colmenares","year":"2004","journal-title":"J. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.4049\/jimmunol.170.4.1635","article-title":"Cutting edge: carbohydrate profiling identifies new pathogens that interact with dendritic cell-specific ICAM-3-grabbing nonintegrin on dendritic cells","volume":"170","author":"Appelmelk","year":"2003","journal-title":"J. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1002\/immu.200310029","article-title":"The C-type lectin DC-SIGN (CD209) is an antigen-uptake receptor for Candida albicans on dendritic cells","volume":"33","author":"Cambi","year":"2003","journal-title":"Eur. J. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1084\/jem.20041061","article-title":"Helicobacter pylori modulates the T helper cell 1\/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN","volume":"200","author":"Bergman","year":"2004","journal-title":"J. Exp. Med."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1023\/A:1025084413767","article-title":"Murine macrophages cultured with IL-4 acquire a phenotype similar to that of epithelioid cells from granulomatous inflammation","volume":"27","author":"Cipriano","year":"2003","journal-title":"Inflammation"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.4049\/jimmunol.170.3.1174","article-title":"IFN-\u03b1\u03b2 released by Mycobacterium tuberculosis-infected human dendritic cells induces the expression of CXCL10: selective recruitment of NK and activated T cells","volume":"170","author":"Lande","year":"2003","journal-title":"J. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/S0171-2985(11)80441-9","article-title":"Immunohistochemical analysis of cell composition and in situ cytokine expression in HIV- and non-HIV-associated tuberculous lymphadenitis","volume":"191","author":"Muller","year":"1994","journal-title":"Immunobiology"},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.4049\/jimmunol.170.4.1939","article-title":"Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells","volume":"170","author":"Tailleux","year":"2003","journal-title":"J. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.4049\/jimmunol.168.5.2118","article-title":"The dendritic cell-specific adhesion receptor DC-SIGN internalizes antigen for presentation to T cells","volume":"168","author":"Engering","year":"2002","journal-title":"J. Immunol."},{"key":"2023033022484310000_","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0165-2478(01)00190-0","article-title":"Inhibition of IL-12 production in human dendritic cells matured in the presence of Bacillus Calmette-Guerin or lipoarabinomannan","volume":"77","author":"Johansson","year":"2001","journal-title":"Immunol. Lett."}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/78\/1\/106\/49647155\/jlb0106.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/78\/1\/106\/49647155\/jlb0106.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T04:36:53Z","timestamp":1706157413000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/78\/1\/106\/6922464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,21]]},"references-count":44,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2005,4,21]]},"published-print":{"date-parts":[[2005,4,21]]}},"URL":"https:\/\/doi.org\/10.1189\/jlb.0105037","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2005,7]]},"published":{"date-parts":[[2005,4,21]]}}}