{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T10:46:44Z","timestamp":1775645204156,"version":"3.50.1"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2008,6,24]],"date-time":"2008-06-24T00:00:00Z","timestamp":1214265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monocytes of bone marrow (BM) origin are circulating precursors that replenish dendritic cells and macrophage populations in peripheral tissues during homeostasis. The eye provides a unique range of varying tissue microenvironments in which to compare the different turnover rates of monocyte-derived cells. This was investigated in the present study using radiation chimeras, whereby BM from Cx3cr1+\/gfp mice was used to rescue myeloablated wild-type (WT) BALB\/c mice (conventional chimeras). The use of Cx3cr1+\/gfp mice as BM donors allowed the clear visualization of newly recruited monocyte-derived cells. Following BM reconstitution, mice were killed at 2, 4, 6, and 8 weeks, and wholemount ocular tissues were processed for immunohistochemistry and confocal microscopy. \u201cReverse\u201d chimeras (WT into Cx3cr1+\/gfp) were also created to act as a further method of cross-referencing cell turnover rates. In conventional chimeras, Cx3cr1+\/gfp cells began repopulating the uveal tract (iris, ciliary body, choroid) 2 weeks post-transplantation with close to complete replenishment by 8 weeks. By contrast, the earliest recruitment of Cx3cr1+\/gfp cells into the host retina occurred at 4 weeks. In reverse chimeras, a steady accumulation of host Cx3cr1+\/gfp macrophages in the subretinal space of Cx3cr1+\/gfp adult mice suggests that these cells arise from long-term resident microglia and not newly recruited WT donor cells. In summary, chimeric mouse models, in which lineage-specific cells carry a fluorescent reporter, have been used in the present study to visualize the turnover of monocyte-derived cells in different tissue compartments of the eye. These data provide valuable insights into differential monocyte turnover rates within a single complex organ.<\/jats:p>","DOI":"10.1189\/jlb.0308166","type":"journal-article","created":{"date-parts":[[2008,6,25]],"date-time":"2008-06-25T02:58:31Z","timestamp":1214362711000},"page":"721-729","source":"Crossref","is-referenced-by-count":53,"title":["Differential turnover rates of monocyte-derived cells in varied ocular tissue microenvironments"],"prefix":"10.1093","volume":"84","author":[{"given":"Jelena","family":"Kezic","sequence":"first","affiliation":[{"name":"School of Anatomy and Human Biology, The University of Western Australia , Perth, Western Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul G","family":"McMenamin","sequence":"additional","affiliation":[{"name":"School of Anatomy and Human Biology, The University of Western Australia , Perth, Western Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,6,24]]},"reference":[{"key":"2023032712464753600_","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/eye.1997.49","article-title":"The distribution of immune cells in the uveal tract of the normal eye","volume":"11","author":"McMenamin","year":"1997","journal-title":"Eye"},{"key":"2023032712464753600_","first-page":"2423","article-title":"Morphologic and ultrastructural examination of I-A+ cells in the murine iris","volume":"32","author":"Knisely","year":"1991","journal-title":"Invest. 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