{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:33:01Z","timestamp":1791127981374,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T00:00:00Z","timestamp":1229040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","award":["17790252"],"award-info":[{"award-number":["17790252"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myeloid DNAX activation protein 12 (DAP12)-associating lectin-1 (MDL-1), also known as C-type lectin domain family 5, member A, is a type II transmembrane protein belonging to the C-type lectin family and associates with DAP12 (also called KARAP or TYROBP). It has been reported that two isoforms of MDL-1\u2014long form (MDL-1L) and short form (MDL-1S)\u2014exist in mice. Previously, we observed the marked induction of MDL-1 mRNA expression during the pulmonary mycobacterial infection in mice. The data suggested that the MDL-1-expressing cells were involved in immune responses against mycobacterial infection; however, little is known about the function of MDL-1 as yet. In this study, we demonstrated the significant protein expression of MDL-1L and MDL-1S in mouse neutrophils and macrophages. MDL-1L was highly glycosylated by N-linked glycan and sialic acid. Interestingly, the expression pattern of MDL-1 was different between neutrophils and macrophages. MDL-1 expression was notably induced during the differentiation of the mouse myeloid cell line 32Dcl3 into neutrophils. Additionally, we observed that MDL-1 stimulation induced a significant amount of RANTES and macrophage-derived chemokine production in 32Dcl3 cells in cooperation with signaling through TLR. MDL-1 stimulation also up-regulated CD11b expression and maintained cell survival. Our findings indicate that MDL-1, therefore, plays an important role in immune defense as a result of an innate immunity, which involves neutrophils and macrophages.<\/jats:p>","DOI":"10.1189\/jlb.0508329","type":"journal-article","created":{"date-parts":[[2008,12,13]],"date-time":"2008-12-13T02:48:19Z","timestamp":1229136499000},"page":"508-517","source":"Crossref","is-referenced-by-count":37,"title":["Expression and functional role of MDL-1 (CLEC5A) in mouse myeloid lineage cells"],"prefix":"10.1093","volume":"85","author":[{"given":"Naoko","family":"Aoki","sequence":"first","affiliation":[{"name":"Department of Pathology , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Pathology , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoji","family":"Kimura","sequence":"additional","affiliation":[{"name":"School of Nursing, Asahikawa Medical College , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Nagato","sequence":"additional","affiliation":[{"name":"Department of Pathology , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Azumi","sequence":"additional","affiliation":[{"name":"Department of Pathology , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroya","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Pathology , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Pathology , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatoshi","family":"Tateno","sequence":"additional","affiliation":[{"name":"Department of Pathology , Asahikawa , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,12,12]]},"reference":[{"key":"2023032912435337400_","doi-asserted-by":"crossref","first-page":"9792","DOI":"10.1073\/pnas.96.17.9792","article-title":"Myeloid DAP12-associating lectin (MDL)-1 is a cell surface receptor involved in the activation of myeloid cells","volume":"96","author":"Bakker","year":"1999","journal-title":"Proc. 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