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It is striking that cytokine expression in the absence of serum and apoptotic cells is equivalent to that of nonautoimmune mice. Here, we show that m\u03c6 from prediseased lupus-prone MRL\/MpJ (MRL\/+) or MRL\/MpJ-Tnfrsf6lpr (MRL\/lpr) mice also have reversible abnormalities in morphology, cytoskeletal organization, and adhesive properties. In the presence of serum, MRL m\u03c6 adhered in increased numbers to a variety of extracellular matrix proteins compared with m\u03c6 from two nonautoimmune strains. However, in the absence of serum, adhesion by MRL m\u03c6 was similar to that of nonautoimmune m\u03c6. Increased adhesion by MRL m\u03c6 was also observed in the presence of apoptotic, but not necrotic, cells. The morphology and actin-staining pattern of adherent MRL m\u03c6 were consistent with reduced activity of Rho, a cytoskeletal regulator. Indeed, MRL m\u03c6 cultured in the presence of serum had markedly decreased levels of active Rho compared with nonautoimmune m\u03c6. It is remarkable that when cultured in the absence of serum, MRL m\u03c6 displayed normal Rho activity and cytoskeletal morphology. Addition of a Rho inhibitor to normal m\u03c6 reproduced the morphologic and cytoskeletal abnormalities observed in MRL m\u03c6. Taken together, our findings support the hypothesis that m\u03c6 from MRL and other systemic lupus erythematosus-prone mice have an apoptotic, cell-dependent, autoimmune phenotype that affects a broad range of m\u03c6 functions, including cytokine gene expression and Rho-dependent cytoskeletal regulation.<\/jats:p>","DOI":"10.1189\/jlb.0604346","type":"journal-article","created":{"date-parts":[[2004,8,18]],"date-time":"2004-08-18T00:58:07Z","timestamp":1092790687000},"page":"971-984","source":"Crossref","is-referenced-by-count":11,"title":["Macrophages from lupus-prone MRL mice are characterized by abnormalities in Rho activity, cytoskeletal organization, and adhesiveness to extracellular matrix proteins"],"prefix":"10.1093","volume":"76","author":[{"given":"Angelika","family":"Longacre","sequence":"first","affiliation":[{"name":"Section of Nephrology, Department of Medicine, The University of Illinois at Chicago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason S","family":"Koh","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Department of Medicine, The University of Illinois at Chicago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin K-H","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Department of Medicine, The University of Illinois at Chicago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Gilligan","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Department of Medicine, The University of Illinois at Chicago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanli","family":"Fan","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Department of Medicine, The University of Illinois at Chicago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vimal A","family":"Patel","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Department of Medicine, The University of Illinois at Chicago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerrold S","family":"Levine","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Department of Medicine, The University of Illinois at Chicago"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,8,17]]},"reference":[{"key":"2023033023455272900_","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1385\/IR:27:1:71","article-title":"Diverse roles of integrins in human T lymphocyte biology","volume":"27","author":"Miyamoto","year":"2003","journal-title":"Immunol. 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