{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:43:29Z","timestamp":1790941409014,"version":"4.1.0"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2009,9,16]],"date-time":"2009-09-16T00:00:00Z","timestamp":1253059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Slit2, a potentially powerful anti-inflammatory agent, inhibits polarization and chemotaxis, but not random movement, of primary neutrophils towards diverse chemoattractants, in vitro and in vivo.<\/jats:p>\n               <jats:p>In inflammatory diseases, circulating neutrophils are recruited to sites of injury. Attractant signals are provided by many different chemotactic molecules, such that blockade of one may not prevent neutrophil recruitment effectively. The Slit family of secreted proteins and their transmembrane receptor, Robo, repel axonal migration during CNS development. Emerging evidence shows that by inhibiting the activation of Rho-family GTPases, Slit2\/Robo also inhibit migration of other cell types toward a variety of chemotactic factors in vitro and in vivo. The role of Slit2 in inflammation, however, has been largely unexplored. We isolated primary neutrophils from human peripheral blood and mouse bone marrow and detected Robo-1 expression. Using video-microscopic live cell tracking, we found that Slit2 selectively impaired directional migration but not random movement of neutrophils toward fMLP. Slit2 also inhibited neutrophil migration toward other chemoattractants, namely C5a and IL-8. Slit2 inhibited neutrophil chemotaxis by preventing chemoattractant-induced actin barbed end formation and cell polarization. Slit2 mediated these effects by suppressing inducible activation of Cdc42 and Rac2 but did not impair activation of other major kinase pathways involved in neutrophil migration. We further tested the effects of Slit2 in vivo using mouse models of peritoneal inflammation induced by sodium periodate, C5a, and MIP-2. In all instances, Slit2 reduced neutrophil recruitment effectively (P&amp;lt;0.01). Collectively, these data demonstrate that Slit2 potently inhibits chemotaxis but not random motion of circulating neutrophils and point to Slit2 as a potential new therapeutic for preventing localized inflammation.<\/jats:p>","DOI":"10.1189\/jlb.0609391","type":"journal-article","created":{"date-parts":[[2009,9,17]],"date-time":"2009-09-17T02:01:50Z","timestamp":1253152910000},"page":"1403-1415","source":"Crossref","is-referenced-by-count":81,"title":["The axonal repellent, Slit2, inhibits directional migration of circulating neutrophils"],"prefix":"10.1093","volume":"86","author":[{"given":"Soumitra","family":"Tole","sequence":"first","affiliation":[{"name":"The Hospital for Sick Children Research Institute , Toronto, Canada"},{"name":"Institute of Medical Science, University of Toronto , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya M","family":"Mukovozov","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children Research Institute , Toronto, Canada"},{"name":"Institute of Medical Science, University of Toronto , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children Research Institute , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A O","family":"Magalhaes","sequence":"additional","affiliation":[{"name":"Canadian Institutes of Health Research Group in Matrix Dynamics, University of Toronto , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yan","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children Research Institute , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Rui","family":"Crow","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children Research Institute , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children Research Institute , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun Xiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Canadian Institutes of Health Research Group in Matrix Dynamics, University of Toronto , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Durocher","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, National Research Council Canada , Montreal, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Glogauer","sequence":"additional","affiliation":[{"name":"Canadian Institutes of Health Research Group in Matrix Dynamics, University of Toronto , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A","family":"Robinson","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children Research Institute , Toronto, Canada"},{"name":"Institute of Medical Science, University of Toronto , Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,9,16]]},"reference":[{"key":"2023032912515446600_jlb1403-cit-b1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1186\/cc2881","article-title":"Bench-to-bedside review: acute respiratory distress syndrome\u2014how neutrophils migrate into the lumg","volume":"8","author":"Reutershan","year":"2004","journal-title":"Crit. 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Purif."},{"key":"2023032912515446600_jlb1403-cit-b24","doi-asserted-by":"crossref","first-page":"15705","DOI":"10.1021\/bi051292p","article-title":"Purification and characterization of a recombinant G-protein-coupled receptor, Saccharomyces cerevisiae Ste2p, transiently expressed in HEK203 EBNA1 cells","volume":"44","author":"Shi","year":"2005","journal-title":"Biochemistry"},{"key":"2023032912515446600_jlb1403-cit-b25","doi-asserted-by":"crossref","first-page":"E9","DOI":"10.1093\/nar\/30.2.e9","article-title":"High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells","volume":"30","author":"Durocher","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"2023032912515446600_jlb1403-cit-b26","doi-asserted-by":"crossref","first-page":"4272","DOI":"10.4049\/jimmunol.165.8.4272","article-title":"Divergent responses to LPS and bacteria in CD14-deficient murine macrophages","volume":"165","author":"Moore","year":"2000","journal-title":"J. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b27","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.cellimm.2003.09.010","article-title":"The chemokine CX3CL1 regulates NK cell activity in vivo","volume":"225","author":"Robinson","year":"2003","journal-title":"Cell. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b28","doi-asserted-by":"crossref","first-page":"6067","DOI":"10.4049\/jimmunol.165.11.6067","article-title":"A role for fractalkine and its receptor (CX3CR1) in cardiac allograft rejection","volume":"165","author":"Robinson","year":"2000","journal-title":"J. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b29","doi-asserted-by":"crossref","first-page":"5769","DOI":"10.4049\/jimmunol.178.9.5769","article-title":"Coronin function is required for chemotaxis and phagocytosis in human neutrophils","volume":"178","author":"Yan","year":"2007","journal-title":"J. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b30","doi-asserted-by":"crossref","first-page":"5908","DOI":"10.1128\/IAI.68.10.5908-5913.2000","article-title":"Modulation of neutrophil chemokine receptors by Staphylococcus aureus supernate","volume":"68","author":"Veldkamp","year":"2000","journal-title":"Infect. Immun."},{"key":"2023032912515446600_jlb1403-cit-b31","doi-asserted-by":"crossref","first-page":"7557","DOI":"10.4049\/jimmunol.176.12.7557","article-title":"Bacillus anthracis toxins inhibit human neutrophil NADPH oxidase activity","volume":"176","author":"Crawford","year":"2006","journal-title":"J. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b32","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1080\/15476910701385612","article-title":"Palladium attenuates the pro-inflammatory interactions of C5a, interleukin-8 and pneumolysin with human neutrophils","volume":"4","author":"Fickl","year":"2007","journal-title":"J. Immunotoxicol."},{"key":"2023032912515446600_jlb1403-cit-b33","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1189\/jlb.0207126","article-title":"Expression and translocation of fluorescent-tagged p21-activated kinase-binding domain and PH domain of protein kinase B during murine neutrophil chemotaxis","volume":"82","author":"Magalhaes","year":"2007","journal-title":"J. Leukoc. Biol."},{"key":"2023032912515446600_jlb1403-cit-b34","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1083\/jcb.200208179","article-title":"Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis","volume":"160","author":"Srinivasan","year":"2003","journal-title":"J. Cell Biol."},{"key":"2023032912515446600_jlb1403-cit-b35","doi-asserted-by":"crossref","first-page":"C526","DOI":"10.1152\/ajpcell.00219.2007","article-title":"Na+\/H+ exchange and pH regulation in the control of neutrophil chemokinesis and chemotaxis","volume":"294","author":"Hayashi","year":"2008","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2023032912515446600_jlb1403-cit-b36","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1016\/S0960-9822(02)01334-9","article-title":"Spatial and temporal analysis of Rac activation during live cell chemotaxis","volume":"12","author":"Gardiner","year":"2002","journal-title":"Curr. Biol."},{"key":"2023032912515446600_jlb1403-cit-b37","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1083\/jcb.150.4.785","article-title":"Two pathways through Cdc42 couple the N-formyl receptor to actin nucleation in permeabilized human neutrophils","volume":"150","author":"Glogauer","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023032912515446600_jlb1403-cit-b38","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1083\/jcb.134.2.389","article-title":"Coordinated regulation of platelet actin filament barbed ends by gelsolin and capping protein","volume":"134","author":"Barkalow","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023032912515446600_jlb1403-cit-b39","doi-asserted-by":"crossref","first-page":"13198","DOI":"10.1074\/jbc.274.19.13198","article-title":"Characterization of Rac and Cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases","volume":"274","author":"Benard","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2023032912515446600_jlb1403-cit-b40","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1084\/jem.20031636","article-title":"Chemotaxis inhibitory protein of Staphylcoccus aureus, a bacterial antiinflammatory agent","volume":"199","author":"De Haas","year":"2004","journal-title":"J. Exp. Med."},{"key":"2023032912515446600_jlb1403-cit-b41","doi-asserted-by":"crossref","first-page":"G1188","DOI":"10.1152\/ajpgi.2001.281.5.G1188","article-title":"Effects of CXC chemokines on neutrophil activation and sequestration in hepatic vasculature","volume":"281","author":"Bajt","year":"2001","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"2023032912515446600_jlb1403-cit-b42","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/S0014-5793(02)02904-6","article-title":"Temporal and spatial expression of two isoforms of the Dutt1\/Robo1 gene in mouse development","volume":"523","author":"Clark","year":"2002","journal-title":"FEBS Lett."},{"key":"2023032912515446600_jlb1403-cit-b43","doi-asserted-by":"crossref","first-page":"5652","DOI":"10.4049\/jimmunol.170.11.5652","article-title":"Rac1 deletion in mouse neutrophils has selective effects on neutrophil function","volume":"170","author":"Glogauer","year":"2003","journal-title":"J. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b44","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S0962-8924(01)02008-6","article-title":"How is actin polymerization nucleated in vivo?","volume":"11","author":"Condeelis","year":"2001","journal-title":"Trends Cell Biol."},{"key":"2023032912515446600_jlb1403-cit-b45","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0960-9822(01)00629-7","article-title":"Cofilin produces newly polymerized actin filaments that are preferred for dendritic nucleation by the Arp\/3 complex","volume":"12","author":"Ichetovkin","year":"2002","journal-title":"Curr. Biol."},{"key":"2023032912515446600_jlb1403-cit-b46","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ceb.2005.11.005","article-title":"Cofilin phosphatases and regulation of actin dynamics","volume":"18","author":"Huang","year":"2006","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023032912515446600_jlb1403-cit-b47","doi-asserted-by":"crossref","first-page":"5043","DOI":"10.4049\/jimmunol.169.9.5043","article-title":"Chemoattractant-stimulated Rac activation in wild-type and Rac2-deficient murine neutrophils: preferential activation of Rac1 and Rac2 gene dosage effect on neutrophil functions","volume":"169","author":"Li","year":"2002","journal-title":"J. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b48","doi-asserted-by":"crossref","first-page":"20983","DOI":"10.1016\/S0021-9258(19)36882-6","article-title":"Translocation of Rac correlates with NADPH oxidase activation. Evidence for equimolar translocation of oxidase components","volume":"268","author":"Quinn","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023032912515446600_jlb1403-cit-b49","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/S0898-6568(03)00074-3","article-title":"MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophils","volume":"15","author":"Coxon","year":"2003","journal-title":"Cell. Signal."},{"key":"2023032912515446600_jlb1403-cit-b50","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.1073\/pnas.052010699","article-title":"Neutrophils lacking phosphoinositide 3-kinase y show loss of directionality during N-formyl-Met-Leu-Phe-induced chemotaxis","volume":"99","author":"Hannigan","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023032912515446600_jlb1403-cit-b51","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1083\/jcb.200202114","article-title":"An intracellular signaling hierarchy determines direction of migration in opposing gradients","volume":"159","author":"Heit","year":"2002","journal-title":"J. CellBiol."},{"key":"2023032912515446600_jlb1403-cit-b52","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.4049\/jimmunol.160.4.1982","article-title":"p38 mitogen-activated protein kinase activation is required for human neutrophil function triggered by TNF-\u03b1 or fMLP stimulation","volume":"160","author":"Zu","year":"1998","journal-title":"J. Immunol."},{"key":"2023032912515446600_jlb1403-cit-b53","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0167-5273(02)00189-4","article-title":"Oxidative stress and neutrophil activation\u2014the two keystones of ischemia\/reperfusion injury","volume":"86","author":"Kaminski","year":"2002","journal-title":"Int. J. Cardiol."},{"key":"2023032912515446600_jlb1403-cit-b54","doi-asserted-by":"crossref","first-page":"5296","DOI":"10.1021\/bi972592c","article-title":"Glucosylation and ADP ribosylation of Rho proteins: effects of nucleotide binding, GTPase activity, and effector coupling","volume":"37","author":"Sehr","year":"1998","journal-title":"Biochemistry"},{"key":"2023032912515446600_jlb1403-cit-b55","doi-asserted-by":"crossref","first-page":"e38","DOI":"10.1371\/journal.pbio.0040038","article-title":"Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis","volume":"4","author":"Weiner","year":"2006","journal-title":"PLoS Biol."},{"key":"2023032912515446600_jlb1403-cit-b56","doi-asserted-by":"crossref","first-page":"e221","DOI":"10.1371\/journal.pbio.0050221","article-title":"An actin-based wave generator organizes cell motility","volume":"5","author":"Weiner","year":"2007","journal-title":"PLoS Biol."},{"key":"2023032912515446600_jlb1403-cit-b57","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1126\/science.1088485","article-title":"Hematopoietic cell regulation by Rac1 and Rac2 guanosine triphosphatases","volume":"302","author":"Gu","year":"2003","journal-title":"Science"},{"key":"2023032912515446600_jlb1403-cit-b58","doi-asserted-by":"crossref","first-page":"29141","DOI":"10.1074\/jbc.M100240200","article-title":"Characterization of Slit protein interactions with glypican-1","volume":"276","author":"Ronca","year":"2001","journal-title":"J. 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