{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:30:17Z","timestamp":1762101017073},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2010,2,9]],"date-time":"2010-02-09T00:00:00Z","timestamp":1265673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Sophia Foundation for Medical Research","award":["#218"],"award-info":[{"award-number":["#218"]}]},{"name":"Stichting Kinderoncologie Rotterdam, the Histiocytosis Association of America and Stichting LCH Nederland"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,2,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mouse histiocytosis sarcoma virus infection induces a heterogeneous disease with characteristics of M\u03c6\/DC neoplasms involving Langerin+ DC, M\u03c6, and precursors.<\/jats:p>\n               <jats:p>Neoplastic diseases of macrophages (M\u03c6) and dendritic cells (DC), collectively called histiocytoses, are relatively rare. The etiology of most forms of histiocytosis is poorly understood, and the development of animal models is crucial for further research in this field. Previously, an animal model for malignant histiocytosis (MH), involving transformed histiocytic cells, has been generated by infecting mice with malignant histiocytosis sarcoma virus (MHSV). However, increased insight into the heterogeneity of M\u03c6 and DC, and the associated reappraisal of human proliferative diseases involving these cells inspired us to re-evaluate the mouse model. We analyzed spleen, bone marrow, and lymph nodes of susceptible mice at various time points after infection. From day 11 onwards, a heterogeneous population of cells, consisting of CD8\u03b1+ Langerin+ DC, ER-MP58+ CD11b+ myeloid precursor cells, CD169+ metallophilic M\u03c6, and CD71hi erythroblasts, was affected by viral transformation. In different mice, these subsets expanded at different rates in different organs, causing a variable disease profile in terminal stages. Cell lines, which were generated from MHSV-transformed tumors, showed a DC-like morphology and phenotype, and appeared to be arrested in different stages of maturation. Upon injection into healthy mice, different preferential homing patterns were observed for the various cell lines, and the cells acquired distinct phenotypes depending on the organ of homing. This indicates that these transformed cells adapt to their microenvironment by switching between precursor, DC\/Langerhans cell, and M\u03c6 phenotypes. Our results demonstrate that the MHSV model represents a heterogeneous neoplastic disease with characteristics of M\u03c6\/DC sarcomas.<\/jats:p>","DOI":"10.1189\/jlb.0609432","type":"journal-article","created":{"date-parts":[[2010,2,10]],"date-time":"2010-02-10T05:14:14Z","timestamp":1265778854000},"page":"949-958","source":"Crossref","is-referenced-by-count":5,"title":["Heterogeneity in a mouse model of histiocytosis: transformation of Langerin+ dendritic cells, macrophages, and precursors"],"prefix":"10.1093","volume":"87","author":[{"given":"Pieter J M","family":"Leenen","sequence":"first","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gitanjali I","family":"Bechan","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , Rotterdam, The Netherlands"},{"name":"Department of Pediatrics, Leiden University Medical Center , Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marleen","family":"Melis","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berlinda J H","family":"den Broeder","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"L\u00f6hler","sequence":"additional","affiliation":[{"name":"Heinrich-Pette-Institute of Experimental Virology and Immunology, University of Hamburg , Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R Maarten","family":"Egeler","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Leiden University Medical Center , Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,2,9]]},"reference":[{"key":"2023020611492716600_B1","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/nrc1632","article-title":"The Nikolas Symposia and histiocytosis","volume":"5","author":"Beverley","year":"2005","journal-title":"Nat. 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