{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:32:55Z","timestamp":1762144375846,"version":"3.37.3"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2005,5,1]],"date-time":"2005-05-01T00:00:00Z","timestamp":1114905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo a Pesquisas do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The phosphatidylinositol-3 kinase (PI3K) family of signaling enzymes plays a crucial role in leukocyte recruitment and activation and hence, likely regulates the induction and propagation phases of inflammation. However, little data have emerged showing a role for these processes in the resolution phase in models of in vivo inflammation. Here, we have evaluated the role of PI3K for the migration and survival of eosinophils in a model of allergic pleurisy in mice. Eosinophil accumulation in PI3K\u03b3-deficient mice was inhibited at 48 h, as compared with wild-type mice but not at earlier time-points (6 and 24 h). Experiments with adoptive transfer of bone marrow showed that PI3K\u03b3 in eosinophils but not in non-bone marrow-derived cells was required for their accumulation. Systemic treatment with PI3K inhibitors before antigen challenge prevented the recruitment of eosinophils. This was associated with decreased Akt phosphorylation, interleukin-5 production, and eosinophil release from the bone marrow. Treatment with PI3K inhibitors 24 h after antigen challenge markedly cleared the accumulated eosinophils, an effect associated with inhibition of Akt phosphorylation and an increased number of apoptotic events. Altogether, our data demonstrate an important role of PI3K\u03b3 for the maintenance of eosinophilic inflammation in vivo, whereas other isoforms of PI3K may be relevant for the recruitment process.<\/jats:p>","DOI":"10.1189\/jlb.0704386","type":"journal-article","created":{"date-parts":[[2005,4,28]],"date-time":"2005-04-28T22:28:06Z","timestamp":1114727286000},"page":"800-810","source":"Crossref","is-referenced-by-count":70,"title":["Phosphoinositide-3 kinases critically regulate the recruitment and survival of eosinophils in vivo: importance for the resolution of allergic inflammation"],"prefix":"10.1093","volume":"77","author":[{"given":"Vanessa","family":"Pinho","sequence":"first","affiliation":[{"name":"Immunopharmacology, Departamento de Bioqu\u00edmica e Imunologia, ICB, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle G","family":"Souza","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departamento de Bioqu\u00edmica e Imunologia, ICB, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele M","family":"Barsante","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departamento de Bioqu\u00edmica e Imunologia, ICB, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana P","family":"Hamer","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departamento de Bioqu\u00edmica e Imunologia, ICB, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta S","family":"De Freitas","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Instituto de Biologia, Universidade do Estado do Rio de Janeiro , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano G","family":"Rossi","sequence":"additional","affiliation":[{"name":"Respiratory Medicine Unit, MRC, Centre for Inflammation Research, Univerity of Edinburgh Medical School , Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro M","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departamento de Bioqu\u00edmica e Imunologia, ICB, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2005,5,1]]},"reference":[{"key":"2023033023021169200_","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1002\/bies.950160810","article-title":"Phosphatidylinositol 3-kinase","volume":"16","author":"Kapeller","year":"1994","journal-title":"Bioessays"},{"key":"2023033023021169200_","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1006\/excr.1999.4701","article-title":"Signaling by distinct classes of phosphoinositide 3-kinases","volume":"253","author":"Vanhaesebroeck","year":"1999","journal-title":"Exp. 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