{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T15:21:22Z","timestamp":1772292082479,"version":"3.50.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2004,10,26]],"date-time":"2004-10-26T00:00:00Z","timestamp":1098748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["NC6987-3"],"award-info":[{"award-number":["NC6987-3"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004240","name":"Academy of Sciences of the Czech Republic","doi-asserted-by":"publisher","award":["A1065203"],"award-info":[{"award-number":["A1065203"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004240","name":"Academy of Sciences of the Czech Republic","doi-asserted-by":"publisher","award":["KSK 5052113"],"award-info":[{"award-number":["KSK 5052113"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004240","name":"Academy of Sciences of the Czech Republic","doi-asserted-by":"publisher","award":["Z5004920"],"award-info":[{"award-number":["Z5004920"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004240","name":"Academy of Sciences of the Czech Republic","doi-asserted-by":"publisher","award":["S5004010"],"award-info":[{"award-number":["S5004010"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Agency of the Czech Republic","doi-asserted-by":"publisher","award":["GA202\/02\/0804"],"award-info":[{"award-number":["GA202\/02\/0804"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","award":["ME565"],"award-info":[{"award-number":["ME565"]}],"id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,10,26]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We show that common heterochromatin antigenic protein markers [HP1\u03b1, -\u03b2, -\u03b3 and mono-, di-, and trimethylated histone H3 lysine 9 (H3K9)], although present in human blood progenitor CD34+ cells, differentiated lymphocytes, and monocytes, are absent in neutrophil granulocytes and to large extent, in eosinophils. Monomethylated and in particular, dimethylated H3K9 are present to variable degrees in the granulocytes of chronic myeloid leukemia (CML) patients, without being accompanied by HP1 proteins. In patients with an acute phase of CML and in acute myeloid leukemia patients, strong methylation of H3K9 and all isoforms of HP1 are detected. In chronic forms of CML, no strong correlations among the level of histone methylation, disease progression, and modality of treatment were observed. Histone methylation was found even in \u201ccured\u201d patients without Philadelphia chromosome (Ph) resulting from +(9;22)(q34;q11) BCR\/ABL translocation, suggesting an incomplete process of developmentally regulated chromatin remodeling in the granulocytes of these patients. Similarly, reprogramming of leukemia HL-60 cells to terminal differentiation by retinoic acid does not eliminate H3K9 methylation and the presence of HP1 isoforms from differentiated granulocytes. Thus, our study shows for the first time that histone H3 methylation may be changed dramatically during normal cell differentiation. The residual histone H3 methylation in myeloid leukemia cells suggests an incomplete chromatin condensation that may be linked to the leukemia cell proliferation and may be important for the prognosis of disease treatment and relapse.<\/jats:p>","DOI":"10.1189\/jlb.0704388","type":"journal-article","created":{"date-parts":[[2004,10,27]],"date-time":"2004-10-27T01:47:52Z","timestamp":1098841672000},"page":"100-111","source":"Crossref","is-referenced-by-count":32,"title":["Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities"],"prefix":"10.1093","volume":"77","author":[{"given":"Emilie","family":"Luk\u00e1\u0161ov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Biophysics, Academy of Sciences of the Czech Republic , Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zden\u011bk","family":"Ko\u0159istek","sequence":"additional","affiliation":[{"name":"Department of Internal Haematooncology , Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Falk","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, Academy of Sciences of the Czech Republic , Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanislav","family":"Kozubek","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, Academy of Sciences of the Czech Republic , Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergei","family":"Grigoryev","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine , Hershey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Kozubek","sequence":"additional","affiliation":[{"name":"Faculty of Informatics, Masaryk University , Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladan","family":"Ond\u0159ej","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, Academy of Sciences of the Czech Republic , Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Kroupov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Masaryk Universit Hospital , Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,10,26]]},"reference":[{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1073\/pnas.50.6.1026","article-title":"Repressed and active chromatin isolated from interphase lymphocytes","volume":"50","author":"Frenster","year":"1963","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1126\/science.1064027","article-title":"Requirement of heterochromatin for cohesion at centromeres","volume":"294","author":"Bernard","year":"2001","journal-title":"Science."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0092-8674(01)00542-6","article-title":"Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability","volume":"107","author":"Peters","year":"2001","journal-title":"Cell"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/ncb739","article-title":"Recruitment of cohesin to heterochromatic regions by Swi6\/HP1 in fission yeast","volume":"4","author":"Nonaka","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1073\/pnas.232688099","article-title":"RNA interference machinery regulates chromosome dynamics during mitosis and meiosis in fission yeast","volume":"100","author":"Hall","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1093\/genetics\/146.4.1221","article-title":"A recombinationally repressed region between mat2 and mat3 loci shares homology to centromeric repeats and regulates directionality of mating-type switching in fusion yeast","volume":"146","author":"Grewal","year":"1997","journal-title":"Genetics"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1101\/gad.14.9.1021","article-title":"Sir2 links chromatin silencing, metabolism, and aging","volume":"14","author":"Guarente","year":"2000","journal-title":"Genes Dev."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1038\/35065132","article-title":"Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins","volume":"410","author":"Lachner","year":"2001","journal-title":"Nature"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1038\/35065138","article-title":"Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromodomain","volume":"410","author":"Bannister","year":"2001","journal-title":"Nature"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/S1097-2765(03)00479-9","article-title":"Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains","volume":"12","author":"Rice","year":"2003","journal-title":"Mol. Cell"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1126\/science.1078572","article-title":"Maintenance of stable heterochromatin domains by dynamic HP1 binding","volume":"299","author":"Cheutin","year":"2003","journal-title":"Science"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1126\/science.1078694","article-title":"Modulation of heterochromatin protein 1 dynamics in primary mammalian cells","volume":"299","author":"Festenstein","year":"2003","journal-title":"Science"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/35040083","article-title":"Nuclear compartmentalization and gene activity","volume":"1","author":"Francastel","year":"2000","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"5626","DOI":"10.1074\/jbc.274.9.5626","article-title":"MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member","volume":"274","author":"Grigoryev","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"5540","DOI":"10.1093\/emboj\/cdg520","article-title":"Formation of facultative heterochromatin in the absence of HP1","volume":"22","author":"Gilbert","year":"2003","journal-title":"EMBO J."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/ng789","article-title":"Histone H3 lysine 9 methylation is an epigenetic imprint of facultative heterochromatin","volume":"30","author":"Peters","year":"2002","journal-title":"Nat. Genet."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/0092-8674(85)90267-3","article-title":"Assembly and propagation of repressed and depressed chromosomal states","volume":"42","author":"Weintraub","year":"1985","journal-title":"Cell"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1242\/jcs.103.4.889","article-title":"Histone H1 and transcription: still an enigma?","volume":"103","author":"Zlatanova","year":"1992","journal-title":"J. Cell Sci."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1126\/science.2740916","article-title":"Histone H5 in the control of DNA synthesis and cell proliferation","volume":"245","author":"Sun","year":"1989","journal-title":"Science"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"6434","DOI":"10.1073\/pnas.92.14.6434","article-title":"Mice develop normally without the H1(0) linker histone","volume":"92","author":"Sirotkin","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0092-8674(95)90051-9","article-title":"Linker histones are not essential and affect chromatin condensation in vivo","volume":"82","author":"Shen","year":"1995","journal-title":"Cell"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/S0092-8674(00)80120-8","article-title":"Linker histone H1 regulates specific gene expression but not global transcription in vivo","volume":"86","author":"Shen","year":"1996","journal-title":"Cell"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"3082","DOI":"10.1074\/jbc.273.5.3082","article-title":"Chromatin structure in granulocytes. A link between tight compaction and accumulation of a heterochromatin-associated protein (MENT)","volume":"273","author":"Grigoryev","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"6455","DOI":"10.1128\/MCB.23.18.6455-6468.2003","article-title":"Insulation of the chicken \u03b2-globin chromosomal domain from a chromatin-condensing protein, MENT","volume":"23","author":"Istomina","year":"2003","journal-title":"Mol. Cell. Biol."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"11949","DOI":"10.1073\/pnas.211322798","article-title":"Distinctive higher-order chromatin structure at mammalian centromeres","volume":"98","author":"Gilbert","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s00412-002-0182-8","article-title":"Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals","volume":"111","author":"Cowell","year":"2002","journal-title":"Chromosoma"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/S0962-8924(01)02001-3","article-title":"Re-SET-ing heterochromatin by histone methyltransferases","volume":"11","author":"Jenuwein","year":"2001","journal-title":"Trends Cell Biol."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"4742","DOI":"10.1073\/pnas.93.10.4742","article-title":"The control of hematopoiesis and leukemia: from basic biology to the clinic","volume":"93","author":"Sachs","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1006\/excr.1998.4210","article-title":"Retinoic acid induction of nuclear envelope-limited chromatin sheets in HL-60","volume":"245","author":"Olins","year":"1998","journal-title":"Exp. Cell Res."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1006\/excr.2001.5269","article-title":"Nuclear envelope and chromatin compositional differences comparing undifferentiated and retinoic acid- and phorbol ester-treated HL-60 cells","volume":"268","author":"Olins","year":"2001","journal-title":"Exp. Cell Res."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.4049\/jimmunol.162.6.3491","article-title":"Differential regulation of 4E-BP1 and 4E-BP2, two repressors of translation initiation, during human myeloid cell differentiation","volume":"162","author":"Grolleau","year":"1999","journal-title":"J. Immunol."},{"key":"2023033023030753500_","volume-title":"Current Protocols in Immunology","author":"Coligan","year":"1991"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/(SICI)1097-0320(19990801)36:4<279::AID-CYTO2>3.0.CO;2-G","article-title":"High resolution cytometry of FISH dots in interphase cell nuclei","volume":"36","author":"Kozubek","year":"1999","journal-title":"Cytometry"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/1097-0320(20010901)45:1<1::AID-CYTO1138>3.0.CO;2-M","article-title":"Combined confocal and wide-field high resolution cytometry of fluorescent in situ hybridization-stained cells","volume":"45","author":"Kozubek","year":"2001","journal-title":"Cytometry"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1038\/35065138","article-title":"Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain","volume":"410","author":"Bannister","year":"2001","journal-title":"Nature"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/nature722","article-title":"Structure of HP1 chromodomain bound to histone H3 methylated at lysine 9","volume":"416","author":"Nielsen","year":"2002","journal-title":"Nature"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1172\/JCI119480","article-title":"Aortic endothelial cells regulate proliferation of human monocytes in vitro via a mechanism synergistic with macrophage colony-stimulating factor. Convergence at the cyclin E\/p27(Kip1) regulatory checkpoint","volume":"99","author":"Antonov","year":"1997","journal-title":"J. Clin. Invest."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S1534-5807(02)00135-1","article-title":"Conserved organization of centromeric chromatin in flies and humans","volume":"2","author":"Blower","year":"2002","journal-title":"Dev. Cell"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"7266","DOI":"10.1073\/pnas.130189697","article-title":"Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro","volume":"97","author":"Yoda","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1096\/fasebj.8.15.8001738","article-title":"Histone H1 zero: a major player in cell differentiation?","volume":"8","author":"Zlatanova","year":"1994","journal-title":"FASEB J."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/S0014-5793(04)00258-3","article-title":"Keeping fingers crossed: heterochromatin spreading through interdigitation of nucleosome arrays","volume":"564","author":"Grigoryev","year":"2004","journal-title":"FEBS Lett."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/S0962-8924(00)89038-8","article-title":"Chromatin organization reviewed","volume":"5","author":"Woodcock","year":"1995","journal-title":"Trends Cell Biol."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S1097-2765(03)00010-8","article-title":"Structural and dynamic functions establish chromatin domains","volume":"11","author":"Ishii","year":"2003","journal-title":"Mol. Cell"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/ng1146","article-title":"Tissue-specific nuclear architecture and gene expression regulated by SATB1","volume":"34","author":"Cai","year":"2003","journal-title":"Nat. Genet."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"2554","DOI":"10.1016\/S0006-3495(98)77963-9","article-title":"Chromatin fiber structure: morphology, molecular determinants, structural transitions","volume":"74","author":"Zlatanov","year":"1998","journal-title":"Biophys. J."},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1182\/blood.V58.1.158.158","article-title":"Evidence for a multistep pathogenesis of chronic myelogenous leukemia","volume":"58","author":"Fialkow","year":"1981","journal-title":"Blood"},{"key":"2023033023030753500_","first-page":"1163","article-title":"Further evidence for the existence of a clonal Ph-negative stage in some cases of Ph-positive chronic myelocytic leukemia","volume":"7","author":"Raskind","year":"1993","journal-title":"Leukemia"},{"key":"2023033023030753500_","first-page":"587","volume-title":"Subcellular Biochemistry","author":"Ye","year":"1998"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1182\/blood.V90.2.803","article-title":"Neutrophil secondary-granule deficiency as a hallmark of all-trans retinoic acid\u2014induced differentiation of acute promyelocytic leukemia cells","volume":"90","author":"Miyauchi","year":"1997","journal-title":"Blood"},{"key":"2023033023030753500_","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil extracellular traps kill bacteria","volume":"303","author":"Brinkmann","year":"2004","journal-title":"Science"}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1189%2Fjlb.0704388","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/77\/1\/100\/49653074\/jlb0100.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/77\/1\/100\/49653074\/jlb0100.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T18:25:19Z","timestamp":1682792719000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/77\/1\/100\/6926465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10,26]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2004,10,26]]},"published-print":{"date-parts":[[2004,10,26]]}},"URL":"https:\/\/doi.org\/10.1189\/jlb.0704388","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2005,1]]},"published":{"date-parts":[[2004,10,26]]}}}