{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:45:06Z","timestamp":1787021106820,"version":"build-2736575974"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2003,11,11]],"date-time":"2003-11-11T00:00:00Z","timestamp":1068508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Charles Wolfson Charitable Trust"},{"DOI":"10.13039\/501100000328","name":"Leukaemia Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000328","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000289","name":"Cancer Research\u2013UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, Portugal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"A University of London Trust Postgraduate Studentship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cyclin D2 affects B cell proliferation and differentiation in vivo. It is rate-limiting for B cell receptor (BCR)-dependent proliferation of B cells, and cyclin D2\u2212\/\u2212 mice lack CD5+(B1) B lymphocytes. We show here that the bone marrow (BM) of cyclin D2\u2212\/\u2212 mice contains half the numbers of Sca1+B220+ B cell progenitors but normal levels of Sca1+ progenitor cells of other lineages. In addition, clonal analysis of BM from the cyclin D2\u2212\/\u2212 and cyclin D2+\/+ mice confirmed that there were fewer B cell progenitors (B220+) in the cyclin D2\u2212\/\u2212 mice. In addition, the colonies from cyclin D2\u2212\/\u2212 mice were less mature (CD19lo) than those from cyclin D2+\/+ mice (CD19Hi). The number of mature B2 B cells in vivo is the same in cyclin D2\u2212\/\u2212 and cyclin D2+\/+ animals. Lack of cyclin D2 protein may be compensated by cyclin D3, as cyclin-dependent kinase (cdk)6 coimmunoprecipitates with cyclin D3 but not cyclin D1 from BM mononuclear cells of cyclin D2\u2212\/\u2212 mice. It is active, as endogenous retinoblastoma protein is phosphorylated at the cdk6\/4-cyclin D-specific sites, S807\/811. We conclude that cyclin D2 is rate-limiting for the production of B lymphoid progenitor cells whose proliferation does not depend on BCR signaling.<\/jats:p>","DOI":"10.1189\/jlb.0803363","type":"journal-article","created":{"date-parts":[[2003,11,17]],"date-time":"2003-11-17T20:12:45Z","timestamp":1069099965000},"page":"1139-1143","source":"Crossref","is-referenced-by-count":20,"title":["<i>Cyclin D2<\/i>\n                    controls B cell progenitor numbers"],"prefix":"10.1093","volume":"74","author":[{"given":"Azim","family":"Mohamedali","sequence":"first","affiliation":[{"name":"Department of Haematological Medicine, Leukaemia Sciences, Guy\u2019s, King\u2019s, St. Thomas\u2019 School of Medicine, Rayne Institute , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00eas","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Cancer Research\u2013UK Labs and Section of Cancer Cell Biology, Imperial College School of Medicine at Hammersmith Hospital , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas C","family":"Lea","sequence":"additional","affiliation":[{"name":"Department of Haematological Medicine, Leukaemia Sciences, Guy\u2019s, King\u2019s, St. Thomas\u2019 School of Medicine, Rayne Institute , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Glassford","sequence":"additional","affiliation":[{"name":"Cancer Research\u2013UK Labs and Section of Cancer Cell Biology, Imperial College School of Medicine at Hammersmith Hospital , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lolita","family":"Banerji","sequence":"additional","affiliation":[{"name":"Cancer Research\u2013UK Labs and Section of Cancer Cell Biology, Imperial College School of Medicine at Hammersmith Hospital , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghulam J","family":"Mufti","sequence":"additional","affiliation":[{"name":"Department of Haematological Medicine, Leukaemia Sciences, Guy\u2019s, King\u2019s, St. Thomas\u2019 School of Medicine, Rayne Institute , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric W-F","family":"Lam","sequence":"additional","affiliation":[{"name":"Cancer Research\u2013UK Labs and Section of Cancer Cell Biology, Imperial College School of Medicine at Hammersmith Hospital , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N Shaun B","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Haematological Medicine, Leukaemia Sciences, Guy\u2019s, King\u2019s, St. Thomas\u2019 School of Medicine, Rayne Institute , London , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,11,11]]},"reference":[{"key":"2023033100222903600_","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1126\/science.7528940","article-title":"Functional isolation and characterization of human hematopoietic stem cells","volume":"267","author":"Berardi","year":"1995","journal-title":"Science"},{"key":"2023033100222903600_","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/nm0296-190","article-title":"Evidence that hematopoiesis may be a stochastic process in vivo","volume":"2","author":"Abkowitz","year":"1996","journal-title":"Nat. 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