{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:18:51Z","timestamp":1759331931276,"version":"3.37.3"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2005,7,8]],"date-time":"2005-07-08T00:00:00Z","timestamp":1120780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GR 534","SFB 547\/B8"],"award-info":[{"award-number":["GR 534","SFB 547\/B8"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,7,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In Wegener's granulomatosis (WG), a pathogenetic role has been proposed for circulating anti-neutrophil-cytoplasmic antibodies (ANCA) targeting proteinase 3 (PR3). Disease activation in WG appears to be triggered by bacterial infections. In the present study, we characterized the effect of anti-PR3 antibodies on in vitro activation of isolated monocytes and neutrophils by the bacterial cell-wall components lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Although sole incubation of monocytes and neutrophils with monoclonal anti-PR3 antibodies induced the release of minor quantities of the chemokine interleukin-8 (IL-8), preincubation with anti-PR3 antibodies, but not with isotype-matched control immunogloblin G (IgG), resulted in a markedly enhanced IL-8 liberation upon LPS challenge. The priming response was evident after 2 h of preincubation with anti-PR3 and peaked after 6 h. The anti-PR3-related priming was also observed for tumor necrosis factor \u03b1 (TNF-\u03b1) and IL-6 synthesis. Comparable priming occurred when leukocytes were preincubated with ANCA-IgG derived from WG serum but not with normal IgG. The priming effect of the anti-PR3 antibody pretreatment was reproduced for LTA challenge of monocytes and neutrophils but not for leukocyte stimulation with TNF-\u03b1. Flow cytometric analysis revealed an increase in monocyte and neutrophil membrane CD14 expression during the anti-PR3 priming. We conclude that cytoplasmic ANCA specifically prime CD14-dependent monocytes and neutrophils for activation. The resulting enhanced responsiveness to bacterial pathogens may contribute to the development and maintenance of inflammatory lesions during active WG.<\/jats:p>","DOI":"10.1189\/jlb.0902442","type":"journal-article","created":{"date-parts":[[2005,7,9]],"date-time":"2005-07-09T00:25:34Z","timestamp":1120868734000},"page":"992-1000","source":"Crossref","is-referenced-by-count":24,"title":["Anti-proteinase 3 antibodies (c-ANCA) prime CD14-dependent leukocyte activation"],"prefix":"10.1093","volume":"78","author":[{"given":"Katja","family":"Hattar","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"van B\u00fcrck","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Bickenbach","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Grandel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Maus","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"},{"name":"Hannover Medical School, Department of Internal Medicine , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Lohmeyer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Csernok","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Medical University of L\u00fcbeck , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Hartung","sequence":"additional","affiliation":[{"name":"Department of Biochemical Pharmacology, University of Konstanz , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Seeger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich","family":"Grimminger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf","family":"Sibelius","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Justus-Liebig-University , Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2005,7,8]]},"reference":[{"key":"2023033022373283500_","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/S0272-6386(12)80874-2","article-title":"Antineutrophil cytoplasmic autoantibody-associated diseases: a pathologist's perspective","volume":"18","author":"Jennette","year":"1991","journal-title":"Am. 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