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A macrophage-specific c-fms promoter was used to express enhanced green fluorescent protein and a drug-inducible suicide gene that leads to Fas-mediated apoptosis in resting and cycling cells of the macrophage lineage. Transgenic mice were fertile, of normal weight, and showed no abnormal phenotype before drug exposure. The transgene was expressed constitutively in macrophages and dendritic cells (DC) but not significantly in T cells or B cells. Induction of the suicide gene led to depletion of 70\u201395% of macrophages and DC in nearly all tissues examined. Depletion reduced the ability to clear bacteria from the blood and led to increased bacterial growth in the liver. Depleted mice displayed several abnormalities, including splenomegaly, lymphadenopathy, thymic atrophy, extramedullary hematopoiesis, and development of peritoneal adhesions. This new, transgenic line will be useful in investigating the role of macrophages and DC.<\/jats:p>","DOI":"10.1189\/jlb.0903442","type":"journal-article","created":{"date-parts":[[2004,1,19]],"date-time":"2004-01-19T20:13:19Z","timestamp":1074543199000},"page":"612-623","source":"Crossref","is-referenced-by-count":275,"title":["Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene"],"prefix":"10.1093","volume":"75","author":[{"given":"Sandra H","family":"Burnett","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine , Lexington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward J","family":"Kershen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine , Lexington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine , Lexington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine , Lexington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan C","family":"Straley","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine , Lexington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan M","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine , Lexington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald A","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine , Lexington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,1,14]]},"reference":[{"key":"2023033100132236500_","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/S0171-2985(84)80144-8","article-title":"Protection of mice against infection with mouse hepatitis virus type 3 by injection of silica","volume":"166","author":"Schindler","year":"1984","journal-title":"Immunobiology"},{"key":"2023033100132236500_","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0022-1759(89)90180-4","article-title":"In vivo depletion of macrophages by desulfated iota-carrageenan in mice","volume":"124","author":"Ishizaka","year":"1989","journal-title":"J. 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