{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:29:12Z","timestamp":1788870552932,"version":"build-2803163510"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Novartis Stiftung f\u00fcr Therapeutische Forschung for financial"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Phytohemagglutinin (PHA) elicited expression of peroxisome proliferator-activated receptor \u03b3 (PPAR\u03b3) in primary human T cells via the PPAR\u03b33 promoter, as shown by reverse transcription-polymerase chain reaction. Electrophoretic mobility shift assay demonstrated no correlation between PPAR\u03b3 expression and its activation. However, addition of specific PPAR\u03b3 agonists such as ciglitazone or 15-deoxy-\u039412,14-prostaglandin J2 (15d-PGJ2) for 1 h following PHA pretreatment provoked PPAR\u03b3 activation verified by supershift analysis. Taking the proapoptotic properties of PPAR\u03b3 into consideration, we analyzed induction of apoptosis in activated T cells in response to PPAR\u03b3 agonists. Cells exposed to PPAR\u03b3 agonists alone revealed minor cell death compared with controls, whereas treatment with 15d-PGJ2 or ciglitazone for 4 h subsequent to PHA stimulation significantly increased cell demise, which was attenuated by the pan-caspase inhibitor zVAD, pointing to apoptosis as the underlying mechanism. These data may be relevant for pathophysiological conditions accompanied with lymphopenia of T cells under conditions such as sepsis.<\/jats:p>","DOI":"10.1189\/jlb.1002487","type":"journal-article","created":{"date-parts":[[2003,4,24]],"date-time":"2003-04-24T15:20:31Z","timestamp":1051197631000},"page":"665-672","source":"Crossref","is-referenced-by-count":20,"title":["Activation-induced PPAR\u03b3 expression sensitizes primary human T cells toward apoptosis"],"prefix":"10.1093","volume":"73","author":[{"given":"Anja","family":"Tautenhahn","sequence":"first","affiliation":[{"name":"University of Kaiserslautern, Institute of Cell Biology , Erwin-Schroedinger-Strasse , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Br\u00fcne","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern, Institute of Cell Biology , Erwin-Schroedinger-Strasse , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"von Knethen","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern, Institute of Cell Biology , Erwin-Schroedinger-Strasse , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,5,1]]},"reference":[{"key":"2023033019044515900_jlb0665-cit-B1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2174\/1566524024605671","article-title":"Regulation of T cell apoptosis during the immune response","volume":"2","author":"Baumann","year":"2002","journal-title":"Curr. 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