{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:02:39Z","timestamp":1760983359315,"version":"3.37.3"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2010,1,22]],"date-time":"2010-01-22T00:00:00Z","timestamp":1264118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100014226","name":"William Harvey Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,1,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Stimulus-specific role for \u03b15\u03b21 in the process of leukocyte recruitment; fibronectin levels are increased in the inflamed tissue so that interaction with \u03b15\u03b21 becomes functional.<\/jats:p>\n               <jats:p>The potential role of \u03b15\u03b21 (VLA-5) in leukocyte trafficking in zymosan-induced acute peritonitis was determined. In na\u00efve mice, \u223c98% of Gr1high cells (PMN) in bone marrow and circulation were \u03b15\u03b21-negative; these profiles were modestly affected by peritoneal injection of zymosan. In contrast, \u223c30% of Gr1high cells recruited by zymosan (24 h) to the peritoneal cavity expressed \u03b15\u03b21. With respect to F4\/80+ cells, \u223c60% of bone marrow and peripheral blood populations expressed \u03b15\u03b21, with \u223c90% positivity in resident cells of noninflamed peritoneum. Analysis of \u03b15\u03b21 expression revealed inflammation-dependent increased expression on Gr1high and F4\/80+ cells in bone marrow, blood, and peritoneal cavity. Blockade of \u03b15\u03b21, by an anti-\u03b15 mAb, attenuated zymosan-induced 24 h recruitment of Gr1high and F4\/80+ cells. At least one underlying mechanism of this action was reduction of cell adhesion and transmigration across microvascular vessels, as revealed by intravital microscopy. Confocal analyses indicated that deposition of fibronectin, the principal ligand for \u03b15\u03b21, was up-regulated significantly on and around the inflamed mesenteric microvasculature. These data suggest that the effects of \u03b15-blockade may be a result of inhibition of \u03b15\u03b21-dependent leukocyte adhesion to and migration along the fibronectin matrix. This is the first report that identifies a functional role for \u03b15\u03b21 in leukocyte trafficking during acute inflammation.<\/jats:p>","DOI":"10.1189\/jlb.1009670","type":"journal-article","created":{"date-parts":[[2010,1,23]],"date-time":"2010-01-23T03:03:14Z","timestamp":1264215794000},"page":"877-884","source":"Crossref","is-referenced-by-count":9,"title":["Inflammation-dependent \u03b15\u03b21 (very late antigen-5) expression on leukocytes reveals a functional role for this integrin in acute peritonitis"],"prefix":"10.1093","volume":"87","author":[{"given":"Andr\u00e9 L F","family":"Sampaio","sequence":"first","affiliation":[{"name":"Barts and The London School of Medicine, Queen Mary University of London , London, United Kingdom"},{"name":"FarManguinhos\u2013FIOCRUZ , Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grit","family":"Zahn","sequence":"additional","affiliation":[{"name":"Jerini AG , Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Leoni","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine, Queen Mary University of London , London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doerte","family":"Vossmeyer","sequence":"additional","affiliation":[{"name":"Jerini AG , Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Christner","sequence":"additional","affiliation":[{"name":"Jerini AG , Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F","family":"Marshall","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine, Queen Mary University of London , London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Perretti","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine, Queen Mary University of London , London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,1,22]]},"reference":[{"key":"2023020611491146000_B1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.it.2005.01.006","article-title":"Transmigration through venular walls: a key regulator of leukocyte phenotype and function","volume":"26","author":"Nourshargh","year":"2005","journal-title":"Trends Immunol."},{"key":"2023020611491146000_B2","first-page":"640","article-title":"Acute lung injury: the role of cytokines in the elicitation of neutrophils","volume":"42","author":"Strieter","year":"1994","journal-title":"J. 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