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The purpose of this study was to investigate the role of TSP-1 in the induction of the Th17 ocular surface response to DS. TSP-1KO and WT mice were subjected to DS5 and DS10), and parameters of ocular surface disease, including corneal barrier function, conjunctival CD4+ T cell infiltration, and GC density, were evaluated. TSP-1KO mice subjected to DS had less corneal barrier disruption, reduced loss of PAS+ GC, and decreased CD4+ T cell infiltration in the conjunctiva. In contrast to WT, TSP-1KO mice failed to up-regulate MMP-3 and MMP-9 mRNA transcripts in the cornea and IL-17A mRNA transcripts in the conjunctiva. RAG-1KO recipients of adoptively transferred CD4+ T cells isolated from TSP-1KO mice subjected to DS5 showed milder dry-eye phenotype and less conjunctival inflammation than recipients of CD4+ T cells from DS5 WT control. Reconstitution of TSP-1KO mice with WT DCs prior to DS reversed the resistance of the TSP-1KO to DS-induced immunopathology. In conclusion, DC-derived TSP-1 is critical for generating the Th17 ocular surface response to DS.<\/jats:p>","DOI":"10.1189\/jlb.1012524","type":"journal-article","created":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T21:33:33Z","timestamp":1377639213000},"page":"1293-1301","source":"Crossref","is-referenced-by-count":29,"title":["Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress"],"prefix":"10.1093","volume":"94","author":[{"given":"Niral B","family":"Gandhi","sequence":"first","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitao","family":"Su","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"},{"name":"Shenyang He Eye Hospital , Shenyang, Liaoning Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene A","family":"Volpe","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia S A","family":"Pelegrino","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salman A","family":"Rahman","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Quan","family":"Li","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen C","family":"Pflugfelder","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cintia S","family":"de Paiva","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,8,27]]},"reference":[{"key":"2023032711174056000_","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1097\/01.ico.0000214802.40313.fa","article-title":"Dysfunctional tear syndrome: a Delphi approach to treatment recommendations","volume":"25","author":"Behrens","year":"2006","journal-title":"Cornea"},{"key":"2023032711174056000_","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.4049\/jimmunol.176.7.3950","article-title":"Desiccating stress induces T cell-mediated Sjogren's syndrome-like lacrimal keratoconjunctivitis","volume":"176","author":"Niederkorn","year":"2006","journal-title":"J. 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