{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:51:28Z","timestamp":1791283888919,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2006,12,18]],"date-time":"2006-12-18T00:00:00Z","timestamp":1166400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>VCAM-1 is a cell surface molecule, which has been shown to mediate leukocyte adhesion to the endothelium and subsequent transmigration. Although VCAM-1 regulates adhesion through its interaction with VLA-4, VLA-4 does not play a role in VCAM-1-dependent diapedesis, an observation suggesting the presence of a second ligand for VCAM-1. We now report a novel interaction between VCAM-1 and secreted protein acidic and rich in cysteine (SPARC), which induces actin cytoskeletal rearrangement and intercellular gaps, physiological processes known to be important for leukocyte transmigration. The binding of leukocyte-derived SPARC to VCAM-1 was demonstrated to be necessary for leukocyte transmigration through endothelial monolayers (diapedesis) in vitro, and furthermore, SPARC null mice have abnormalities in leukocyte recruitment to the inflamed peritoneum in vivo. These findings provide new insight into the mechanisms of transendothelial leukocyte migration and suggest a potential, targetable interaction for therapeutic intervention.<\/jats:p>","DOI":"10.1189\/jlb.1105664","type":"journal-article","created":{"date-parts":[[2006,12,19]],"date-time":"2006-12-19T01:34:41Z","timestamp":1166492081000},"page":"748-756","source":"Crossref","is-referenced-by-count":61,"title":["SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration"],"prefix":"10.1093","volume":"81","author":[{"given":"Kimberly A","family":"Kelly","sequence":"first","affiliation":[{"name":"Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R","family":"Allport","sequence":"additional","affiliation":[{"name":"Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy M","family":"Yu","sequence":"additional","affiliation":[{"name":"Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumita","family":"Sinh","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Massachusetts General Hospital , Charlestown, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E Helene","family":"Sage","sequence":"additional","affiliation":[{"name":"Hope Heart Program, Benaroya Research Institute at Virginia Mason , Seattle, Washington , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E","family":"Gerszten","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Massachusetts General Hospital , Charlestown, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Weissleder","sequence":"additional","affiliation":[{"name":"Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,12,18]]},"reference":[{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/0092-8674(91)90279-8","article-title":"Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity","volume":"67","author":"Butcher","year":"1991","journal-title":"Cell"},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S1471-4906(03)00117-0","article-title":"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response","volume":"24","author":"Muller","year":"2003","journal-title":"Trends Immunol."},{"key":"2023033007394479100_","first-page":"815","article-title":"Alternative splicing of human VCAM-1 in activated vascular endothelium","volume":"138","author":"Cybulsky","year":"1991","journal-title":"Am. J. Pathol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1172\/JCI11871","article-title":"A major role for VCAM-1, but not ICAM-1, in early atherosclerosis","volume":"107","author":"Cybulsky","year":"2001","journal-title":"J. Clin. Invest."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/j.1399-0012.1994.tb00257.x","article-title":"Endothelial activation and chronic allograft rejection","volume":"8","author":"Orosz","year":"1994","journal-title":"Clin. Transplant."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1083\/jcb.128.6.1243","article-title":"The integrin VLA-4 supports tethering and rolling in flow on VCAM-1","volume":"128","author":"Alon","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1083\/jcb.142.5.1381","article-title":"Endothelial cell E- and P-selectin and vascular cell adhesion molecule-1 function as signaling receptors","volume":"142","author":"Lorenzon","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1084\/jem.178.2.449","article-title":"PECAM-1 is required for transendothelial migration of leukocytes","volume":"178","author":"Muller","year":"1993","journal-title":"J. Exp. Med."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"25790","DOI":"10.1016\/S0021-9258(19)74459-7","article-title":"Specific interaction of SPARC with endothelial cells is mediated through a carboxyl-terminal sequence containing a calcium-binding EF hand","volume":"268","author":"Yost","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"3448","DOI":"10.1073\/pnas.91.8.3448","article-title":"SPARC (secreted protein acidic and rich in cysteine) regulates endothelial cell shape and barrier function","volume":"91","author":"Goldblum","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1189\/jlb.0904554","article-title":"Active participation of endothelial cells in inflammation","volume":"77","author":"Cook-Mills","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"6550","DOI":"10.4049\/jimmunol.164.12.6550","article-title":"Lymphocyte migration through monolayers of endothelial cell lines involves VCAM-1 signaling via endothelial cell NADPH oxidase","volume":"164","author":"Matheny","year":"2000","journal-title":"J. Immunol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1002\/jcb.240570218","article-title":"SPARC mediates focal adhesion disassembly in endothelial cells through a follistatin-like region and the Ca(2+)-binding EF-hand","volume":"57","author":"Murphy-Ullrich","year":"1995","journal-title":"J. Cell. Biochem."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1083\/jcb.109.1.341","article-title":"SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix","volume":"109","author":"Sage","year":"1989","journal-title":"J. Cell Biol."},{"key":"2023033007394479100_","first-page":"2674","article-title":"SPARC deficiency leads to early-onset cataractogenesis","volume":"39","author":"Norose","year":"1998","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.4049\/jimmunol.142.7.2257","article-title":"Endothelial-leukocyte adhesion molecule-1-dependent and leukocyte (CD11\/CD18)-dependent mechanisms contribute to polymorphonuclear leukocyte adhesion to cytokine-activated human vascular endothelium","volume":"142","author":"Luscinskas","year":"1989","journal-title":"J. Immunol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.4049\/jimmunol.149.6.2163","article-title":"In vitro inhibitory effect of IL-8 and other chemoattractants on neutrophil-endothelial adhesive interactions","volume":"149","author":"Luscinskas","year":"1992","journal-title":"J. Immunol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1189\/jlb.71.5.821","article-title":"Neutrophils from MMP-9- or neutrophil elastase-deficient mice show no defect in transendothelial migration under flow in vitro","volume":"71","author":"Allport","year":"2002","journal-title":"J. Leukoc. Biol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1161\/01.RES.0000155722.17881.dd","article-title":"Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle","volume":"96","author":"Kelly","year":"2005","journal-title":"Circ. Res."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","DOI":"10.1002\/0471143030.cb1011s17","article-title":"Purification of SPARC\/osteonectin.","author":"Sage","year":"2003"},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"4365","DOI":"10.4049\/jimmunol.158.9.4365","article-title":"L-selectin shedding does not regulate human neutrophil attachment, rolling, or transmigration across human vascular endothelium in vitro","volume":"158","author":"Allport","year":"1997","journal-title":"J. Immunol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1084\/jem.192.3.439","article-title":"BLTR mediates leukotriene B4-induced chemotaxis and adhesion plays a dominant role in eosinophil accumulation in a murine model of peritonitis","volume":"192","author":"Tager","year":"2000","journal-title":"J. Exp. Med."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/S0945-053X(00)00133-5","article-title":"SPARC, a matricellular protein: at the crossroads of cell-matrix communication","volume":"19","author":"Brekken","year":"2001","journal-title":"Matrix Biol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1084\/jem.20030202","article-title":"Leukocyte, rather than tumor-produced SPARC, determines stroma and collagen type IV deposition in mammary carcinoma","volume":"198","author":"Sangaletti","year":"2003","journal-title":"J. Exp. Med."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"5123","DOI":"10.1158\/0008-5472.CAN-04-1102","article-title":"Secreted protein acidic and rich in cysteine produced by human melanoma cells modulates polymorphonuclear leukocyte recruitment and antitumor cytotoxic capacity","volume":"65","author":"Alvarez","year":"2005","journal-title":"Cancer Res."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1002\/(SICI)1097-0045(19970801)32:3<214::AID-PROS8>3.0.CO;2-K","article-title":"Serum levels of endothelial and neural cell adhesion molecules in prostate cancer","volume":"32","author":"Lynch","year":"1997","journal-title":"Prostate"},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1172\/JCI12609","article-title":"The de-adhesive activity of matricellular proteins: is intermediate cell adhesion an adaptive state?","volume":"107","author":"Murphy-Ullrich","year":"2001","journal-title":"J. Clin. Invest."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.4049\/jimmunol.167.4.2323","article-title":"Real-time imaging of vascular endothelial-cadherin during leukocyte transmigration across endothelium","volume":"167","author":"Shaw","year":"2001","journal-title":"J. Immunol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1083\/jcb.148.1.203","article-title":"Monocytes induce reversible focal changes in vascular endothelial cadherin complex during transendothelial migration under flow","volume":"148","author":"Allport","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1182\/blood-2004-12-4942","article-title":"ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-\u03b1-activated vascular endothelium under flow","volume":"106","author":"Yang","year":"2005","journal-title":"Blood"},{"key":"2023033007394479100_","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1172\/JCI16804","article-title":"Enhanced growth of tumors in SPARC null mice is associated with changes in the ECM","volume":"111","author":"Brekken","year":"2003","journal-title":"J. Clin. Invest."},{"key":"2023033007394479100_","first-page":"1231","article-title":"The role of lymphocytes and macrophages in human breast tumorigenesis: an immunohistochemical and morphometric study","volume":"22","author":"Ben-Hur","year":"2002","journal-title":"Anticancer Res."}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1189%2Fjlb.1105664","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/81\/3\/748\/49644514\/jlb0748.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/81\/3\/748\/49644514\/jlb0748.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T17:10:24Z","timestamp":1736701824000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/81\/3\/748\/6975580"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12,18]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2006,12,18]]},"published-print":{"date-parts":[[2007,3,1]]}},"URL":"https:\/\/doi.org\/10.1189\/jlb.1105664","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2007,3]]},"published":{"date-parts":[[2006,12,18]]}}}