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Chemokine receptors transduce intracellular signals by activating heterotrimeric G-proteins. Acting to limit and modulate heterotrimeric G-protein signaling is a family of proteins, termed regulator of G-protein signaling (RGS). Two of these proteins, RGS1 and RGS13, are well-expressed in germinal center B cells and many Burkitt\u2019s lymphoma cell lines. Reducing RGS13 and to a lesser extent RGS1 expression in a Burkitt\u2019s lymphoma cell line enhances responsiveness to two chemokines, CXC chemokine ligand 12 (CXCL12) and CXCL13, and reducing both mRNAs augments the responses more dramatically. The double knock-down (KD) cells respond better to restimulation with CXCL12 or CXCL13 after a primary stimulation with CXCL12 than do the control cells. The double-KD cells also exhibit a greater propensity to polarize and to develop multiple small lamellipodia. These results indicate that RGS1 and RGS13 act together to regulate chemokine receptor signaling in human germinal center B lymphocytes and provide evidence that they contribute significantly to the rapid desensitization of the signaling pathway.<\/jats:p>","DOI":"10.1189\/jlb.1105693","type":"journal-article","created":{"date-parts":[[2006,3,25]],"date-time":"2006-03-25T01:44:25Z","timestamp":1143251065000},"page":"1357-1368","source":"Crossref","is-referenced-by-count":62,"title":["RGS1 and RGS13 mRNA silencing in a human B lymphoma line enhances responsiveness to chemoattractants and impairs desensitization"],"prefix":"10.1093","volume":"79","author":[{"given":"Jang-Il","family":"Han","sequence":"first","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning-Na","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Uk","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H","family":"Kehrl","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,3,24]]},"reference":[{"key":"2023033015234075700_cit-1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0092-8674(00)81521-4","article-title":"The role of chemokines in regulating cell migration during humoral immune responses","volume":"99","author":"Melchers","year":"1999","journal-title":"Cell"},{"key":"2023033015234075700_cit-2","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/S0952-7915(00)00096-0","article-title":"Chemokines in tissue-specific and microenvironment-specific lymphocyte homing","volume":"12","author":"Campbell","year":"2000","journal-title":"Curr. 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