{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T16:49:13Z","timestamp":1776703753453,"version":"3.51.2"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2008,4,21]],"date-time":"2008-04-21T00:00:00Z","timestamp":1208736000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EA055944"],"award-info":[{"award-number":["EA055944"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA101944"],"award-info":[{"award-number":["CA101944"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA114931"],"award-info":[{"award-number":["CA114931"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA095128"],"award-info":[{"award-number":["CA095128"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Induction of active tumor-specific immunity in patients with chronic lymphocytic leukemia (CLL) and other hematologic malignancies is compromised by the deficit of endogenous dendritic cells (DCs). In attempt to develop improved vaccination strategies for patients with CLL and other tumors with poorly identified rejection antigens, we tested the ability of ex vivo-generated DCs to cross-present the antigens expressed by CLL cells and to induce CLL-specific, functional CTL responses. Monocyte-derived DCs from CLL patients were induced to mature using a \u201cstandard\u201d cytokine cocktail (in IL-1\u03b2, TNF-\u03b1, IL-6, and PGE2) or using an \u03b1-type 1-polarized DC (\u03b1DC1) cocktail (in IL-1\u03b2, TNF-\u03b1, IFN-\u03b1, IFN-\u03b3, and polyinosinic:polycytidylic acid) and were loaded with \u03b3-irradiated, autologous CLL cells. \u03b1DC1 from CLL patients expressed substantially higher levels of multiple costimulatory molecules (CD83, CD86, CD80, CD11c, and CD40) than standard DCs (sDCs) and immature DCs, and their expression of CCR7 showed intermediate level. \u03b1DC1 secreted substantially higher (10\u201360 times) levels of IL-12p70 than sDCs. Although \u03b1DC1 and sDCs showed similar uptake of CLL cells, \u03b1DC1 induced much higher numbers (range, 2.4\u201338 times) of functional CD8+ T cells against CLL cells. The current demonstration that autologous tumor-loaded \u03b1DC1 are potent inducers of CLL-specific T cells helps to develop improved immunotherapies of CLL.<\/jats:p>","DOI":"10.1189\/jlb.1107737","type":"journal-article","created":{"date-parts":[[2008,4,22]],"date-time":"2008-04-22T00:35:43Z","timestamp":1208824543000},"page":"319-325","source":"Crossref","is-referenced-by-count":63,"title":["Type 1-polarized dendritic cells loaded with autologous tumor are a potent immunogen against chronic lymphocytic leukemia"],"prefix":"10.1093","volume":"84","author":[{"given":"Je-Jung","family":"Lee","sequence":"first","affiliation":[{"name":"Departments of Surgery and Medicine, University of Pittsburgh, University of Pittsburgh Cancer Institute , Pittsburgh, Pennsylvania , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth A","family":"Foon","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Medicine, University of Pittsburgh, University of Pittsburgh Cancer Institute , Pittsburgh, Pennsylvania , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robbie B","family":"Mailliard","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Medicine, University of Pittsburgh, University of Pittsburgh Cancer Institute , Pittsburgh, Pennsylvania , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravikumar","family":"Muthuswamy","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Medicine, University of Pittsburgh, University of Pittsburgh Cancer Institute , Pittsburgh, Pennsylvania , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawel","family":"Kalinski","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Medicine, University of Pittsburgh, University of Pittsburgh Cancer Institute , Pittsburgh, Pennsylvania , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,4,21]]},"reference":[{"key":"2023032712564693200_","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1182\/blood.V78.8.1901.1901","article-title":"B-cell chronic lymphocytic leukemia: present status and future directions. 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