{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:25:35Z","timestamp":1791221135956,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2003,7,15]],"date-time":"2003-07-15T00:00:00Z","timestamp":1058227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001742","name":"The United States\u2013Israel Binational Science Foundation","doi-asserted-by":"publisher","award":["1999012"],"award-info":[{"award-number":["1999012"]}],"id":[{"id":"10.13039\/501100001742","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Proteinase 3 (PR3), the target autoantigen of antineutrophil cytoplasmic antibodies in the autoimmune vasculitis, Wegener\u2019s granulomatosis, is a serine proteinase stored in granules of human neutrophils. As previously shown, PR3 is expressed also on the plasma membrane of unactivated neutrophils, and this expression increases in primed or stimulated cells. The current study demonstrates that membrane-bound PR3 colocalizes with the adhesion molecule CD11b\/CD18 (\u03b22 integrin). Immunoprecipitation experiments using plasma membranes of phorbol 12-myristate 13-acetate (PMA)-stimulated neutrophils revealed coimmunoprecipitation of PR3 with CD11b\/CD18, indicating their location in the same complex. PR3 was also detected in TritonX-100-insoluble cytoskeleton of plasma membranes isolated from unactivated and activated neutrophils. Release of cytoskeletal PR3 by salt treatment implied electrostatic interaction with the enzyme. The serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF) augmented membrane expression of PR3 in unactivated and PMA-stimulated neutrophils. PMSF significantly reduced adhesion of neutrophils to fibrinogen-coated plates and their NADPH oxidase activity. Moreover, the addition of exogenous PR3 (1\u20135 \u03bcg\/ml) augmented the CD11b\/CD18-dependent adhesion of neutrophils. Taken together, these results implicate the \u03b22 integrin of neutrophils in their membrane association with PR3 and suggest a role of PR3 in the modulation of cell adhesion.<\/jats:p>","DOI":"10.1189\/jlb.1202624","type":"journal-article","created":{"date-parts":[[2003,9,10]],"date-time":"2003-09-10T01:25:10Z","timestamp":1063157110000},"page":"551-557","source":"Crossref","is-referenced-by-count":45,"title":["Interaction of proteinase 3 with CD11b\/CD18 (\u03b22integrin) on the cell membrane of human neutrophils"],"prefix":"10.1093","volume":"74","author":[{"given":"A","family":"David","sequence":"first","affiliation":[{"name":"Department Biochemistry, Tel Aviv University , Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Kacher","sequence":"additional","affiliation":[{"name":"Department Biochemistry, Tel Aviv University , Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"Specks","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Mayo Clinic , Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Aviram","sequence":"additional","affiliation":[{"name":"Department Biochemistry, Tel Aviv University , Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,7,15]]},"reference":[{"key":"2023033100470463000_jlb0551-cit-B1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1189\/jlb.69.2.177","article-title":"Proteinase 3, Wegener\u2019s autoantigen: from gene to antigen","volume":"69","author":"Van der Geld","year":"2001","journal-title":"J. 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