{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:13:13Z","timestamp":1762157593088,"version":"3.37.3"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2004,2,13]],"date-time":"2004-02-13T00:00:00Z","timestamp":1076630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000026","name":"National Institute of Drug Abuse","doi-asserted-by":"crossref","award":["DA07908","DA16828"],"award-info":[{"award-number":["DA07908","DA16828"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cannabinoids exhibit broad immune modulating activity by targeting many cell types within the immune system, including T cells, which exhibit sensitivity, as evidenced by altered activation, proliferation, and cytokine expression. As a result of the critical role calcium plays in T cell function coupled with previous findings demonstrating disruption of the calcium-regulated transcription factor, nuclear factor of activated T cells, by cannabinoid treatment the objective of the present investigation was to perform an initial characterization of the role of the cannabinoid receptors in the regulation of the intracellular calcium concentration ([Ca2+]i) by \u03949-tetrahydrocannabinol (\u03949-THC) in T lymphocytes. Here, we demonstrate that \u03949-THC robustly elevates [Ca2+]i in purified murine splenic T cells and in the human peripheral blood acute lymphoid leukemia (HPB-ALL) human T cell line but only minimally elevates [Ca2+]i in Jurkat E6-1 (dysfunctional cannabinoid receptor 2-expressing) human T cells. Removal of extracellular calcium severely attenuated the \u03949-THC-mediated rise in [Ca2+]i in murine splenic T cells and HPB-ALL cells. Pretreatment with cannabinoid receptor antagonists, SR144528 and\/or SR141716A, led to an attenuation of \u03949-THC-mediated elevation in [Ca2+]i in splenic T cells and HPB-ALL cells but not in Jurkat E6-1 cells. Furthermore, pretreatment of HPB-ALL cells with SR144528 antagonized the small rise in [Ca2+]i elicited by \u03949-THC in the absence of extracellular calcium. These findings suggest that \u03949-THC induces an influx of extracellular calcium in resting T cells in a cannabinoid receptor-dependent manner.<\/jats:p>","DOI":"10.1189\/jlb.1203638","type":"journal-article","created":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T01:34:04Z","timestamp":1076981644000},"page":"884-892","source":"Crossref","is-referenced-by-count":30,"title":["Cannabinoid receptor-mediated regulation of intracellular calcium by \u03949-tetrahydrocannabinol in resting T cells"],"prefix":"10.1093","volume":"75","author":[{"given":"Gautham K","family":"Rao","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University , East Lansing"},{"name":"National Food Safety and Toxicology Center, Michigan State University , East Lansing"},{"name":"Center for Integrative Toxicology, Michigan State University , East Lansing"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University , East Lansing"},{"name":"National Food Safety and Toxicology Center, Michigan State University , East Lansing"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert E","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University , East Lansing"},{"name":"National Food Safety and Toxicology Center, Michigan State University , East Lansing"},{"name":"Center for Integrative Toxicology, Michigan State University , East Lansing"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,2,13]]},"reference":[{"key":"2023033100122694500_","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/S0167-5699(98)01300-0","article-title":"Cannabinoid receptors and immunity","volume":"19","author":"Klein","year":"1998","journal-title":"Immunol. 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