{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:29:18Z","timestamp":1790908158859,"version":"4.1.0"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2005,7,20]],"date-time":"2005-07-20T00:00:00Z","timestamp":1121817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Medical Research Council and Bioscience and Environmental Research Council of the Academy of Finland","award":["80666"],"award-info":[{"award-number":["80666"]}]},{"name":"Medical Research Council and Bioscience and Environmental Research Council of the Academy of Finland","award":["201967"],"award-info":[{"award-number":["201967"]}]},{"name":"Medical Research Council and Bioscience and Environmental Research Council of the Academy of Finland","award":["202505"],"award-info":[{"award-number":["202505"]}]},{"name":"Medical Research Council and Bioscience and Environmental Research Council of the Academy of Finland","award":["205644"],"award-info":[{"award-number":["205644"]}]},{"DOI":"10.13039\/100007797","name":"University of Helsinki","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007797","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Sigrid Juselius Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,7,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Salmonella enterica serovar typhimurium (S. typhimurium) is an intracellular pathogen causing localized gastroenteritis in humans. Macrophages (M\u03c6s) and dendritic cells (DCs) play an important role in innate immunity against Salmonella. In this report, we have compared the consequences of infection of human M\u03c6s and DCs with wild-type S. typhimurium and an isogenic PgtE-defective strain. PgtE is an outer membrane protein hypothesized to have a role in intracellular survival of Salmonella. We observed that DCs undergo full maturation in response to Salmonella infection, as indicated by up-regulation of cell-surface marker proteins CD80, CD83, CD86, and human leukocyte antigen class II. CC chemokine ligand 5 (CCL5), CXC chemokine ligand 10, tumor necrosis factor \u03b1, interleukin (IL)-12, and IL-18 gene expression and protein production were readily induced by Salmonella-infected M\u03c6s and DCs. CCL20 was preferentially produced by M\u03c6s, whereas DCs secreted higher levels of CCL19 as compared with M\u03c6s. DCs and M\u03c6s infected with S. typhimurium also produced high levels of interferon-\u03b3 (IFN-\u03b3). Cytokine neutralization and stimulation experiments suggest that the production was partly regulated by Salmonella-induced type I IFNs, IL-12, and IL-18. DC cytokine production induced by Salmonella was much higher as compared with the responses induced by Salmonella lipopolysaccharide or flagellin. M\u03c6s and DCs were capable of internalizing and harboring Salmonella for several days. S. enterica PgtE provided no survival advantage for the bacteria in human M\u03c6s or DCs. Our results demonstrate that although M\u03c6s and DCs share similar functions, they may have different roles during Salmonella infection as a result of differential production of certain chemokines and cytokines.<\/jats:p>","DOI":"10.1189\/jlb.1204721","type":"journal-article","created":{"date-parts":[[2005,7,20]],"date-time":"2005-07-20T20:24:18Z","timestamp":1121891058000},"page":"909-920","source":"Crossref","is-referenced-by-count":51,"title":["Activation, cytokine production, and intracellular survival of bacteria in\n                    <i>Salmonella<\/i>\n                    -infected human monocyte-derived macrophages and dendritic cells"],"prefix":"10.1093","volume":"78","author":[{"given":"Taija E","family":"Pietil\u00e4","sequence":"first","affiliation":[{"name":"Department of Viral Diseases and Immunology, National Public Health Institute , Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ville","family":"Veckman","sequence":"additional","affiliation":[{"name":"Department of Viral Diseases and Immunology, National Public Health Institute , Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e4ivi","family":"Kyll\u00f6nen","sequence":"additional","affiliation":[{"name":"General Microbiology, Faculty of Biosciences, University of Helsinki , Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaarina","family":"L\u00e4hteenm\u00e4ki","sequence":"additional","affiliation":[{"name":"General Microbiology, Faculty of Biosciences, University of Helsinki , Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo K","family":"Korhonen","sequence":"additional","affiliation":[{"name":"General Microbiology, Faculty of Biosciences, University of Helsinki , Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilkka","family":"Julkunen","sequence":"additional","affiliation":[{"name":"Department of Viral Diseases and Immunology, National Public Health Institute , Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,7,20]]},"reference":[{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1146\/annurev.med.52.1.259","article-title":"Salmonella: a model for bacterial pathogenesis","volume":"52","author":"Ohl","year":"2001","journal-title":"Annu. Rev. Med."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1016\/S1286-4579(01)01478-2","article-title":"The role of M cells in Salmonella infection","volume":"3","author":"Jepson","year":"2001","journal-title":"Microbes Infect."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1078\/0171-2985-00094","article-title":"Dendritic cells shuttle microbes across gut epithelial monolayers","volume":"204","author":"Rescigno","year":"2001","journal-title":"Immunobiology"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"14650","DOI":"10.1073\/pnas.97.26.14650","article-title":"Entry and survival of Salmonella typhimurium in dendritic cells and presentation of recombinant antigens do not require macrophage-specific virulence factors","volume":"97","author":"Niedergang","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1128\/iai.57.5.1517-1523.1989","article-title":"Nucleotide sequence of the plasminogen activator gene of Yersinia pestis: relationship to ompT of Escherichia coli and gene E of","volume":"57","author":"Sodeinde","year":"1989","journal-title":"Salmonella typhimurium. Infect. Immun."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1126\/science.1439793","article-title":"A surface protease and the invasive character of plague","volume":"258","author":"Sodeinde","year":"1992","journal-title":"Science"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1462-5822.2004.00483.x","article-title":"Antiprotease inactivation by Salmonella enterica released from infected macrophages","volume":"7","author":"L\u00e4hteenm\u00e4ki","year":"2005","journal-title":"Cell. Microbiol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"4077","DOI":"10.1128\/JB.182.14.4077-4086.2000","article-title":"A PhoP-regulated outer membrane protease of Salmonella enterica serovar typhimurium promotes resistance to \u03b1-helical antimicrobial peptides","volume":"182","author":"Guina","year":"2000","journal-title":"J. Bacteriol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1046\/j.1365-2958.2003.03313.x","article-title":"Unravelling the biology of macrophage infection by gene expression profiling of intracellular","volume":"47","author":"Eriksson","year":"2003","journal-title":"Salmonella enterica. Mol. Microbiol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1111\/j.1365-2958.2005.04553.x","article-title":"Co-regulation of Salmonella enterica genes required for virulence and resistance to antimicrobial peptides by SlyA and PhoP\/PhoQ","volume":"56","author":"Navarre","year":"2005","journal-title":"Mol. Microbiol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/j.1574-695X.2000.tb01445.x","article-title":"Salmonella infection of bone marrow-derived macrophages and dendritic cells: influence on antigen presentation and initiating an immune response","volume":"27","author":"Yrlid","year":"2000","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1146\/annurev.immunol.18.1.767","article-title":"Immunobiology of dendritic cells","volume":"18","author":"Banchereau","year":"2000","journal-title":"Annu. Rev. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/S1286-4579(01)01479-4","article-title":"Cytokines in host defense against","volume":"3","author":"Eckmann","year":"2001","journal-title":"Salmonella. Microbes Infect."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"2561","DOI":"10.1073\/pnas.041618998","article-title":"Defective localization of the NADPH phagocyte oxidase to Salmonella-containing phagosomes in tumor necrosis factor p55 receptor-deficient macrophages","volume":"98","author":"Vazquez-Torres","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"6133","DOI":"10.4049\/jimmunol.170.12.6133","article-title":"Salmonella typhimurium strains carrying independent mutations display similar virulence phenotypes yet are controlled by distinct host defense mechanisms","volume":"170","author":"Raupach","year":"2003","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S1286-4579(99)80073-2","article-title":"Host cytokine response and resistance to Salmonella infection","volume":"1","author":"Lalmanach","year":"1999","journal-title":"Microbes Infect."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1002\/(SICI)1521-4141(199904)29:04<1107::AID-IMMU1107>3.0.CO;2-0","article-title":"Salmonella efficiently enter and survive within cultured CD11c+ dendritic cells initiating cytokine expression","volume":"29","author":"Marriott","year":"1999","journal-title":"Eur. J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1189\/jlb.69.4.583","article-title":"Genetic background of attenuated Salmonella typhimurium has profound influence on infection and cytokine patterns in human dendritic cells","volume":"69","author":"Dreher","year":"2001","journal-title":"J. Leukoc. Biol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1189\/jlb.72.4.743","article-title":"Salmonella virulence factor SipB induces activation and release of IL-18 in human dendritic cells","volume":"72","author":"Dreher","year":"2002","journal-title":"J. Leukoc. Biol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1046\/j.1365-2958.2003.03817.x","article-title":"Lack of O-antigen is essential for plasminogen activation by Yersinia pestis and Salmonella enterica","volume":"51","author":"Kukkonen","year":"2004","journal-title":"Mol. Microbiol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1189\/jlb.1003461","article-title":"Streptococcus pyogenes and Lactobacillus rhamnosus differentially induce maturation and production of Th1-type cytokines and chemokines in human monocyte-derived dendritic cells","volume":"75","author":"Veckman","year":"2004","journal-title":"J. Leukoc. Biol."},{"key":"2023033022354810900_","first-page":"443","article-title":"Raising antibodies to human leukocyte interferon","volume":"83","author":"Mogensen","year":"1975","journal-title":"Acta Pathol. Microbiol. Scand. [B]"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"7322","DOI":"10.4049\/jimmunol.162.12.7322","article-title":"Virus infection activates IL-1 \u03b2 and IL-18 production in human macrophages by a caspase-1-dependent pathway","volume":"162","author":"Pirhonen","year":"1999","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"5294","DOI":"10.1021\/bi00591a005","article-title":"Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease","volume":"18","author":"Chirgwin","year":"1979","journal-title":"Biochemistry"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"2633","DOI":"10.1021\/bi00709a025","article-title":"Ribonucleic acid isolated by cesium chloride centrifugation","volume":"13","author":"Glisin","year":"1974","journal-title":"Biochemistry"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1006\/viro.2000.0542","article-title":"Influenza A and sendai viruses induce differential chemokine gene expression and transcription factor activation in human macrophages","volume":"276","author":"Matikainen","year":"2000","journal-title":"Virology"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.4049\/jimmunol.158.3.1033","article-title":"Identification through bioinformatics of two new macrophage proinflammatory human chemokines: MIP-3\u03b1 and MIP-3\u03b2","volume":"158","author":"Rossi","year":"1997","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.4049\/jimmunol.158.5.2363","article-title":"Regulation of IFN-\u03b1\/\u03b2, MxA, 2\u2032,5\u2032-oligoadenylate synthetase, and HLA gene expression in influenza A-infected human lung epithelial cells","volume":"158","author":"Ronni","year":"1997","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.1073\/pnas.88.10.4143","article-title":"Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor","volume":"88","author":"Gubler","year":"1991","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"4274","DOI":"10.4049\/jimmunol.156.11.4274","article-title":"Cloning of the cDNA for human IFN-\u03b3-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein","volume":"156","author":"Ushio","year":"1996","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1042\/bj3030831","article-title":"Role of N-glycosylation in the synthesis, dimerization and secretion of human interferon-\u03b3","volume":"303","author":"Sareneva","year":"1994","journal-title":"Biochem. J."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1002\/eji.1830260736","article-title":"Interferon-\u03b3-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-\u03b3 production","volume":"26","author":"Micallef","year":"1996","journal-title":"Eur. J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"3594","DOI":"10.1128\/iai.65.9.3594-3599.1997","article-title":"Interleukin-12 (IL-12) and IL-18 synergistically induce the fungicidal activity of murine peritoneal exudate cells against Cryptococcus neoformans through production of \u03b3 interferon by natural killer cells","volume":"65","author":"Zhang","year":"1997","journal-title":"Infect. Immun."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.4049\/jimmunol.160.12.6032","article-title":"Influenza A virus-induced IFN-\u03b1\/\u03b2 and IL-18 synergistically enhance IFN-\u03b3 gene expression in human T cells","volume":"160","author":"Sareneva","year":"1998","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1002\/1521-4141(200107)31:7<2236::AID-IMMU2236>3.0.CO;2-G","article-title":"IFN-\u03b1 and IL-18 synergistically enhance IFN-\u03b3 production in human NK cells: differential regulation of Stat4 activation and IFN-\u03b3 gene expression by IFN-\u03b1 and IL-12","volume":"31","author":"Matikainen","year":"2001","journal-title":"Eur. J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1126\/science.282.5396.2085","article-title":"Defective LPS signaling in C3H\/HeJ and C57BL\/10ScCr mice: mutations in Tlr4 gene","volume":"282","author":"Poltorak","year":"1998","journal-title":"Science"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/35074106","article-title":"The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5","volume":"410","author":"Hayashi","year":"2001","journal-title":"Nature"},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1084\/jem.175.4.1111","article-title":"Killing of virulent Mycobacterium tuberculosis by reactive nitrogen intermediates produced by activated murine macrophages","volume":"175","author":"Chan","year":"1992","journal-title":"J. Exp. Med."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1128\/IAI.69.2.800-809.2001","article-title":"Fate of Mycobacterium tuberculosis within murine dendritic cells","volume":"69","author":"Bodnar","year":"2001","journal-title":"Infect. Immun."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/84219","article-title":"Lymphocyte traffic control by chemokines","volume":"2","author":"Moser","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1084\/jem.188.2.373","article-title":"Selective recruitment of immature and mature dendritic cells by distinct chemokines expressed in different anatomic sites","volume":"188","author":"Dieu","year":"1998","journal-title":"J. Exp. Med."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1002\/(SICI)1521-4141(199905)29:05<1617::AID-IMMU1617>3.0.CO;2-3","article-title":"Distinct patterns and kinetics of chemokine production regulate dendritic cell function","volume":"29","author":"Sallusto","year":"1999","journal-title":"Eur. J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1006\/mpat.2002.0497","article-title":"Migration of Salmonella typhimurium\u2014harboring bone marrow-derived dendritic cells towards the chemokines CCL19 and CCL21","volume":"32","author":"Cheminay","year":"2002","journal-title":"Microb. Pathog."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1462-5822.2003.00336.x","article-title":"Immunity to Salmonella from a dendritic point of view","volume":"6","author":"Sundquist","year":"2004","journal-title":"Cell. Microbiol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1128\/IAI.71.8.4382-4388.2003","article-title":"Human alveolar macrophages infected by virulent bacteria expressing SipB are a major source of active interleukin-18","volume":"71","author":"Obregon","year":"2003","journal-title":"Infect. Immun."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"5165","DOI":"10.4049\/jimmunol.170.10.5165","article-title":"The Toll-like receptor 5 stimulus bacterial flagellin induces maturation and chemokine production in human dendritic cells","volume":"170","author":"Means","year":"2003","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S1359-6101(98)00007-0","article-title":"IFN-\u03b3 expression in macrophages and its possible biological significance","volume":"9","author":"Gessani","year":"1998","journal-title":"Cytokine Growth Factor Rev."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"5448","DOI":"10.4049\/jimmunol.166.9.5448","article-title":"Differential production of IL-12, IFN-\u03b1, and IFN-\u03b3 by mouse dendritic cell subsets","volume":"166","author":"Hochrein","year":"2001","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"6742","DOI":"10.4049\/jimmunol.171.12.6742","article-title":"Salmonella rapidly kill dendritic cells via a caspase-1-dependent mechanism","volume":"171","author":"van der Velden","year":"2003","journal-title":"J. Immunol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1016\/S1286-4579(01)01493-9","article-title":"Salmonella and apoptosis: to live or let die?","volume":"3","author":"Knodler","year":"2001","journal-title":"Microbes Infect."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1128\/JB.183.8.2445-2453.2001","article-title":"Adhesion of type 1-fimbriated Escherichia coli to abiotic surfaces leads to altered composition of outer membrane proteins","volume":"183","author":"Otto","year":"2001","journal-title":"J. Bacteriol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1128\/JB.186.1.226-234.2004","article-title":"Inactivation of ompX causes increased interactions of type 1 fimbriated Escherichia coli with abiotic surfaces","volume":"186","author":"Otto","year":"2004","journal-title":"J. Bacteriol."},{"key":"2023033022354810900_","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1146\/annurev.mi.49.100195.001323","article-title":"Nonopsonic phagocytosis of microorganisms","volume":"49","author":"Ofek","year":"1995","journal-title":"Annu. Rev. Microbiol."}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1189%2Fjlb.1204721","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/78\/4\/909\/49646789\/jlb0909.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/78\/4\/909\/49646789\/jlb0909.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T18:55:08Z","timestamp":1706381708000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/78\/4\/909\/6922436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7,20]]},"references-count":53,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2005,7,20]]},"published-print":{"date-parts":[[2005,7,20]]}},"URL":"https:\/\/doi.org\/10.1189\/jlb.1204721","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2005,10]]},"published":{"date-parts":[[2005,7,20]]}}}