{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:39:52Z","timestamp":1772894392667,"version":"3.50.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":6061,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 HL71711"],"award-info":[{"award-number":["RO1 HL71711"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"DOD","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DAMD","award":["17-03-1-0004"],"award-info":[{"award-number":["17-03-1-0004"]}]},{"name":"Schmidt Biomedical Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,7,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Previous studies have shown that prostaglandin E2 (PGE2) release by splenic F4\/80+ cyclooxygenase (COX)-2+ macrophages (M\u00d8) isolated from mice, treated with mycobacterial components, plays a major role in the regulation of immune responses. However, splenic M\u00d8, isolated from untreated mice and treated in vitro with lipopolysaccharide and interferon-\u03b3, express COX-1 and COX-2 within 1 day but release only minimal amounts of PGE2 following elicitation with calcium ionophore A23187. For further characterization of in vivo requirements for development of PGE2-releasing M\u00d8 (PGE2-M\u00d8), C57Bl\/6 [wild-type (WT)], and interleukin (IL)-10-deficient (IL-10\u2212\/\u2212) mice were treated intraperitoneally with heat-killed Mycobacterium bovis bacillus Calmette-Guerin (HK-BCG). One day following injection, COX-2 was induced in splenic M\u00d8 of both mouse strains. However, PGE2 biosynthesis by these M\u00d8 was not increased. Thus, expression of COX-2 is not sufficient to induce PGE2 production in vivo or in vitro. In sharp contrast, 14 days after HK-BCG treatment, PGE2 release by COX-2+ splenic M\u00d8 increased as much as sevenfold, and a greater increase was seen in IL-10\u2212\/\u2212 cells than in WT cells. To further determine whether the 14-day splenic PGE2-M\u00d8 could be derived from bone marrow precursors, we established a chimera in which bone marrow cells were transfused from green fluorescent protein (GFP)-transgenic donors to WT mice. Donors and recipients were treated with HK-BCG simultaneously, and marrow transfusion was performed on Days 1 and 2. On Day 14 after BCG treatment, a significant number of spleen cells coexpressed COX-2 and GFP, indicating that bone marrow-derived COX-2+ M\u00d8 may be responsible for the increased PGE2 production.<\/jats:p>","DOI":"10.1189\/jlb.1205737","type":"journal-article","created":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T00:55:01Z","timestamp":1152147301000},"page":"590-598","source":"Crossref","is-referenced-by-count":11,"title":["Heat-killed BCG induces biphasic cyclooxygenase 2+ splenic macrophage formation\u2014role of IL-10 and bone marrow precursors"],"prefix":"10.1093","volume":"80","author":[{"given":"Yoshimi","family":"Shibata","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Florida Atlantic University , Boca Raton"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon","family":"Gabbard","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Florida Atlantic University , Boca Raton"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makiko","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Florida Atlantic University , Boca Raton"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoutaro","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Florida Atlantic University , Boca Raton"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mike","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Physiology, Brody School of Medicine at East Carolina University , Greenville, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihito","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Florida Atlantic University , Boca Raton"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth Ann","family":"Henriksen","sequence":"additional","affiliation":[{"name":"Department of Physiology, Brody School of Medicine at East Carolina University , Greenville, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quentin N","family":"Myrvik","sequence":"additional","affiliation":[{"name":"Caswel Beach , North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,7,5]]},"reference":[{"key":"2023033101031090700_jlb0590-cit-B1","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1084\/jem.181.2.775","article-title":"Prostaglandin-E2 is a potent inhibitor of human interleukin 12 production","volume":"181","author":"Van der Pouw Kraan","year":"1995","journal-title":"J. Exp. Med."},{"key":"2023033101031090700_jlb0590-cit-B2","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1002\/jlb.61.6.712","article-title":"Timing of prostaglandin exposure is critical for the inhibition of LPS- or IFN-\u03b3-induced macrophage NO synthesis by PGE2","volume":"61","author":"Harbrecht","year":"1997","journal-title":"J. Leukoc. Biol."},{"key":"2023033101031090700_jlb0590-cit-B3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0008-8749(87)90197-3","article-title":"Rat macrophage treatment with lipopolysaccharide leads to a reduction in respiratory burst product secretion and a decrease in NADPH oxidase affinity","volume":"108","author":"Johnson","year":"1987","journal-title":"Cell. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B4","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/S0006-2952(01)00586-X","article-title":"Regulation of TNF\u03b1 and interleukin-10 production by prostaglandins I(2) and E(2): studies with prostaglandin receptor-deficient mice and prostaglandin E-receptor subtype-selective synthetic agonists","volume":"61","author":"Shinomiya","year":"2001","journal-title":"Biochem. Pharmacol."},{"key":"2023033101031090700_jlb0590-cit-B5","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1189\/jlb.0605321","article-title":"Splenic PGE2-releasing macrophages regulate Th1 and Th2 immune responses in mice treated with heat-killed BCG","volume":"78","author":"Shibata","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"2023033101031090700_jlb0590-cit-B6","doi-asserted-by":"crossref","first-page":"6955","DOI":"10.4049\/jimmunol.173.11.6955","article-title":"Prostaglandin E2 promotes the survival of bone marrow-derived dendritic cells","volume":"173","author":"Vassiliou","year":"2004","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B7","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.4049\/jimmunol.175.3.1483","article-title":"Prostaglandin E2 induces FOXP3 gene expression and T regulatory cell function in human CD4+ T cells","volume":"175","author":"Baratelli","year":"2005","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B8","first-page":"75","article-title":"Eicosanoid production by peritoneal and splenic macrophages in mice depleted of bone marrow by 89Sr","volume":"127","author":"Shibata","year":"1987","journal-title":"Am. J. Pathol."},{"key":"2023033101031090700_jlb0590-cit-B9","doi-asserted-by":"crossref","first-page":"3897","DOI":"10.4049\/jimmunol.135.6.3897","article-title":"The effect of bone marrow depletion on prostaglandin E-producing suppressor macrophages in mouse spleen","volume":"135","author":"Shibata","year":"1985","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B10","doi-asserted-by":"crossref","first-page":"5743","DOI":"10.1128\/IAI.66.12.5743-5750.1998","article-title":"Mycobacterial dose defines the Th1\/Th2 nature of the immune response independently of whether immunization is administered by the intravenous, subcutaneous, or intradermal route","volume":"66","author":"Power","year":"1998","journal-title":"Infect. Immun."},{"key":"2023033101031090700_jlb0590-cit-B11","first-page":"539","article-title":"Establishment of stable, cell-mediated immunity that makes \u201csusceptible\u201d mice resistant to","volume":"257","author":"Bretscher","year":"1992","journal-title":"Leishmania major. Science"},{"key":"2023033101031090700_jlb0590-cit-B12","doi-asserted-by":"crossref","first-page":"3475","DOI":"10.1128\/iai.60.9.3475-3479.1992","article-title":"The Th1\/Th2-like switch in syphilitic infection: is it detrimental?","volume":"60","author":"Fitzgerald","year":"1992","journal-title":"Infect. Immun."},{"key":"2023033101031090700_jlb0590-cit-B13","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1126\/science.8098553","article-title":"A strategy for prophylactic vaccination against HIV","volume":"260","author":"Salk","year":"1993","journal-title":"Science"},{"key":"2023033101031090700_jlb0590-cit-B14","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1189\/jlb.70.6.849","article-title":"Modes of action of Freund's adjuvants in experimental models of autoimmune diseases","volume":"70","author":"Billiau","year":"2001","journal-title":"J. Leukoc. Biol."},{"key":"2023033101031090700_jlb0590-cit-B15","doi-asserted-by":"crossref","first-page":"108","DOI":"10.4049\/jimmunol.146.1.108","article-title":"Prostaglandin E2 inhibits production of Th1 lymphokines but not of Th2 lymphokines","volume":"146","author":"Betz","year":"1991","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B16","doi-asserted-by":"crossref","first-page":"32783","DOI":"10.1074\/jbc.M003505200","article-title":"Regulation of prostaglandin E2 biosynthesis by inducible membrane-associated prostaglandin E2 synthase that acts in concert with cyclooxygenase-2","volume":"275","author":"Murakami","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2023033101031090700_jlb0590-cit-B17","doi-asserted-by":"crossref","first-page":"5811","DOI":"10.4049\/jimmunol.168.11.5811","article-title":"Lipopolysaccharide-dependent prostaglandin E(2) production is regulated by the glutathione-dependent prostaglandin E(2) synthase gene induced by the Toll-like receptor 4\/MyD88\/NF-IL6 pathway","volume":"168","author":"Uematsu","year":"2002","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B18","first-page":"169","article-title":"Restoration of prostaglandin E2-producing splenic macrophages in 89Sr-treated mice with bone marrow from Corynebacterium parvum primed donors","volume":"2","author":"Shibata","year":"1989","journal-title":"Reg. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B19","doi-asserted-by":"crossref","first-page":"757","DOI":"10.4049\/jimmunol.170.2.757","article-title":"Mechanisms of enhanced macrophage-mediated prostaglandin E2 production and its suppressive role in Th1 activation in Th2-dominant BALB\/c mice","volume":"170","author":"Kuroda","year":"2003","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B20","doi-asserted-by":"crossref","first-page":"2674","DOI":"10.4049\/jimmunol.166.4.2674","article-title":"Il-10 is a central regulator of cyclooxygenase-2 expression and prostaglandin production","volume":"166","author":"Berg","year":"2001","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B21","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1189\/jlb.0504311","article-title":"Differential effects of IL-10 on prostaglandin H synthase-2 expression and prostaglandin E2 biosynthesis between spleen and bone marrow macrophages","volume":"77","author":"Shibata","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"2023033101031090700_jlb0590-cit-B22","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1182\/blood.V89.5.1621","article-title":"Regulation by interleukin-10 and interleukin-4 of cyclooxygenase-2 expression in human neutrophils","volume":"89","author":"Niiro","year":"1997","journal-title":"Blood"},{"key":"2023033101031090700_jlb0590-cit-B23","doi-asserted-by":"crossref","first-page":"3736","DOI":"10.1182\/blood.V85.12.3736.bloodjournal85123736","article-title":"Inhibition by interleukin-10 of inducible cyclooxygenase expression in lipopolysaccharide-stimulated monocytes: its underlying mechanism in comparison with interleukin-4","volume":"85","author":"Niiro","year":"1995","journal-title":"Blood"},{"key":"2023033101031090700_jlb0590-cit-B24","doi-asserted-by":"crossref","first-page":"6123","DOI":"10.1128\/IAI.69.10.6123-6130.2001","article-title":"Th1 adjuvant N-acetyl-D-glucosamine polymer up-regulates Th1 immunity but down-regulates Th2 immunity against a mycobacterial protein (MPB-59) in interleukin-10-knockout and wild-type mice","volume":"69","author":"Shibata","year":"2001","journal-title":"Infect. Immun."},{"key":"2023033101031090700_jlb0590-cit-B25","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0002-9440(10)63942-9","article-title":"Leukocytes expressing green fluorescent protein as novel reagents for adoptive cell transfer and bone marrow transplantation studies","volume":"158","author":"Manfra","year":"2001","journal-title":"Am. J. Pathol."},{"key":"2023033101031090700_jlb0590-cit-B26","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0014-5793(97)00313-X","article-title":"\u201cGreen mice\u201d as a source of ubiquitous green cells","volume":"407","author":"Okabe","year":"1997","journal-title":"FEBS Lett."},{"key":"2023033101031090700_jlb0590-cit-B27","doi-asserted-by":"crossref","first-page":"3316","DOI":"10.1182\/blood.V83.11.3316.3316","article-title":"Macrophage colony-stimulating factor-induced bone marrow macrophages do not synthesize or release prostaglandin E2","volume":"83","author":"Shibata","year":"1994","journal-title":"Blood"},{"key":"2023033101031090700_jlb0590-cit-B28","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1073\/pnas.91.6.2046","article-title":"Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation","volume":"91","author":"Vane","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033101031090700_jlb0590-cit-B29","doi-asserted-by":"crossref","first-page":"2878","DOI":"10.4049\/jimmunol.154.6.2878","article-title":"Prostaglandin E2 release triggered by phagocytosis of latex particles. A distinct association with prostaglandin synthase isozymes in bone marrow macrophages","volume":"154","author":"Shibata","year":"1995","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B30","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1042\/bj3320541","article-title":"Fatty acids induce release of Ca2+ from acidosomal stores and activate capacitative Ca2+ entry in","volume":"332","author":"Schaloske","year":"1998","journal-title":"Dictyostelium discoideum. Biochem. J."},{"key":"2023033101031090700_jlb0590-cit-B31","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1006\/excr.2000.4863","article-title":"Lack of cyclooxygenase-2 activity in HT-29 human colorectal carcinoma cells","volume":"256","author":"Hsi","year":"2000","journal-title":"Exp. Cell Res."},{"key":"2023033101031090700_jlb0590-cit-B32","doi-asserted-by":"crossref","first-page":"15069","DOI":"10.1073\/pnas.93.26.15069","article-title":"Peroxynitrite, the coupling product of nitric oxide and superoxide, activates prostaglandin biosynthesis","volume":"93","author":"Landino","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023033101031090700_jlb0590-cit-B33","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1146\/annurev.biochem.69.1.145","article-title":"Cyclooxygenases: structural, cellular, and molecular biology","volume":"69","author":"Smith","year":"2000","journal-title":"Annu. Rev. Biochem."},{"key":"2023033101031090700_jlb0590-cit-B34","doi-asserted-by":"crossref","first-page":"963","DOI":"10.4049\/jimmunol.163.2.963","article-title":"Lipopolysaccharide modulates cyclooxygenase-2 transcriptionally and posttranscriptionally in human macrophages independently from endogenous IL-1 \u03b2 and TNF-\u03b1","volume":"163","author":"Barrios-Rodiles","year":"1999","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B35","doi-asserted-by":"crossref","first-page":"4930","DOI":"10.4049\/jimmunol.158.10.4930","article-title":"Inhibition of prostaglandin endoperoxide synthase-2 expression in stimulated human monocytes by inhibitors of p38 mitogen-activated protein kinase","volume":"158","author":"Pouliot","year":"1997","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B36","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1006\/bbrc.1998.9287","article-title":"MAP kinase pathways as a route for regulatory mechanisms of IL-10 and IL-4 which inhibit COX-2 expression in human monocytes","volume":"250","author":"Niiro","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2023033101031090700_jlb0590-cit-B37","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1074\/jbc.274.1.264","article-title":"p38 mitogen-activated protein kinase regulates cyclooxygenase-2 mRNA stability and transcription in lipopolysaccharide-treated human monocytes","volume":"274","author":"Dean","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2023033101031090700_jlb0590-cit-B38","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1093\/intimm\/dxf038","article-title":"Molecular mechanisms of lipopolysaccharide-induced cyclooxygenase-2 expression in human neutrophils: involvement of the mitogen-activated protein kinase pathway and regulation by anti-inflammatory cytokines","volume":"14","author":"Nagano","year":"2002","journal-title":"Int. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B39","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.4049\/jimmunol.172.4.2147","article-title":"Novel regulatory mechanisms of CD40-induced prostanoid synthesis by IL-4 and IL-10 in human monocytes","volume":"172","author":"Inoue","year":"2004","journal-title":"J. Immunol."},{"key":"2023033101031090700_jlb0590-cit-B40","doi-asserted-by":"crossref","first-page":"3760","DOI":"10.1093\/emboj\/20.14.3760","article-title":"Interleukin-10 targets p38 MAPK to modulate ARE-dependent TNF mRNA translation and limit intestinal pathology","volume":"20","author":"Kontoyiannis","year":"2001","journal-title":"EMBO J."},{"key":"2023033101031090700_jlb0590-cit-B41","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1172\/JCI5257","article-title":"Selective activation and functional significance of p38\u03b1 mitogen-activated protein kinase in lipopolysaccharide-stimulated neutrophils","volume":"103","author":"Nick","year":"1999","journal-title":"J. Clin. Invest."},{"key":"2023033101031090700_jlb0590-cit-B42","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1046\/j.1365-2567.1996.d01-758.x","article-title":"Neutrophils are involved in the non-specific resistance to listeriosis induced by mycobacterial infections","volume":"89","author":"Leal","year":"1996","journal-title":"Immunology"}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1189%2Fjlb.1205737","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1189\/jlb.1205737","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1189\/jlb.1205737","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/80\/3\/590\/49644189\/jlb0590.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/80\/3\/590\/49644189\/jlb0590.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T02:15:35Z","timestamp":1680228935000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/80\/3\/590\/6922579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,5]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2006,7,5]]},"published-print":{"date-parts":[[2006,7,5]]}},"URL":"https:\/\/doi.org\/10.1189\/jlb.1205737","relation":{},"ISSN":["1938-3673","0741-5400"],"issn-type":[{"value":"1938-3673","type":"electronic"},{"value":"0741-5400","type":"print"}],"subject":[],"published-other":{"date-parts":[[2006,9]]},"published":{"date-parts":[[2006,7,5]]}}}