{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:17:09Z","timestamp":1789514229611,"version":"build-2803163510"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T00:00:00Z","timestamp":1480291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["123403"],"award-info":[{"award-number":["123403"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Canadian Foundation for Innovation","award":["29838"],"award-info":[{"award-number":["29838"]}]},{"name":"McGill University Faculty of Medicine"},{"name":"Hematopoiesis and Lymphocyte Differentiation","award":["950-228977"],"award-info":[{"award-number":["950-228977"]}]},{"DOI":"10.13039\/501100001659","name":"German Research Association","doi-asserted-by":"publisher","award":["DFG FO 900\/1-1"],"award-info":[{"award-number":["DFG FO 900\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019683","name":"Cole Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019683","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"CIHR operating","doi-asserted-by":"publisher","award":["MOP-114972"],"award-info":[{"award-number":["MOP-114972"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Leaders Opportunity Fund infrastructure"},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"American Association of Immunologists Careers in Immunology Fellowship Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MYSM1 is a chromatin-binding histone deubiquitinase. MYSM1 mutations in humans result in lymphopenia whereas loss of Mysm1 in mice causes severe hematopoietic abnormalities, including an early arrest in B cell development. However, it remains unknown whether MYSM1 is required at later checkpoints in B cell development or for B cell\u2013mediated immune responses. We analyzed conditional mouse models Mysm1fl\/flTg.mb1-cre, Mysm1fl\/flTg.CD19-cre, and Mysm1fl\/flTg.CD21-cre with inactivation of Mysm1 at prepro-B, pre-B, and follicular B cell stages of development. We show that loss of Mysm1 at the prepro-B cell stage in Mysm1fl\/flTg.mb1-cre mice results in impaired B cell differentiation, with an \u223c2-fold reduction in B cell numbers in the lymphoid organs. Mysm1fl\/flTg.mb1-cre B cells also showed increased expression of activation markers and impaired survival and proliferation. In contrast, Mysm1 was largely dispensable from the pre-B cell stage onward, with Mysm1fl\/flTg.CD19-cre and Mysm1fl\/flTg.CD21-cre mice showing no alterations in B cell numbers and largely normal responses to stimulation. MYSM1, therefore, has an essential role in B cell lineage specification but is dispensable at later stages of development. Importantly, MYSM1 activity at the prepro-B cell stage of development is important for the normal programming of B cell responses to stimulation once they complete their maturation process<\/jats:p>","DOI":"10.1189\/jlb.1ab0415-177rr","type":"journal-article","created":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T02:50:55Z","timestamp":1480387855000},"page":"643-654","source":"Crossref","is-referenced-by-count":16,"title":["MYSM1-dependent checkpoints in B cell lineage differentiation and B cell\u2013mediated immune response"],"prefix":"10.1093","volume":"101","author":[{"given":"Michael","family":"F\u00f6rster","sequence":"first","affiliation":[{"name":"Department of Physiology, McGill University , Montreal, Quebec , Canada"},{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyo","family":"Farrington","sequence":"additional","affiliation":[{"name":"Department of Physiology, McGill University , Montreal, Quebec , Canada"},{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica C","family":"Petrov","sequence":"additional","affiliation":[{"name":"Department of Physiology, McGill University , Montreal, Quebec , Canada"},{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jad I","family":"Belle","sequence":"additional","affiliation":[{"name":"Department of Physiology, McGill University , Montreal, Quebec , Canada"},{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara C","family":"Mindt","sequence":"additional","affiliation":[{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"},{"name":"Department of Microbiology and Immunology, McGill University , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Witalis","sequence":"additional","affiliation":[{"name":"Department of Physiology, McGill University , Montreal, Quebec , Canada"},{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia U","family":"Duerr","sequence":"additional","affiliation":[{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"},{"name":"Department of Microbiology and Immunology, McGill University , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg H","family":"Fritz","sequence":"additional","affiliation":[{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"},{"name":"Department of Microbiology and Immunology, McGill University , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Nijnik","sequence":"additional","affiliation":[{"name":"Department of Physiology, McGill University , Montreal, Quebec , Canada"},{"name":"McGill University Research Centre on Complex Traits , Montreal, Quebec , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,11,28]]},"reference":[{"key":"2023011712061198200_B1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/nri3801","article-title":"The early history of B cells","volume":"15","author":"Cooper","year":"2015","journal-title":"Nat. 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