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Although FR-targeted therapies aimed at eliminating activated macrophages have shown promise for treating inflammatory diseases, little work has been performed to evaluate whether other hematopoietic cells might also express FR-\u03b2. Analysis of peripheral blood cells with a mAb to human FR-\u03b2 reveals that only monocytes express FR-\u03b2. Molecular characterization of these circulating monocytes further demonstrates that solely the classic\/proinflammatory subset (CD14highCD16\u2212) expresses the FR and that only CD14highCD16\u2212 FR-\u03b2+ monocytes also display the ability to bind folate-linked molecules. Confirmation that this subset of monocytes indeed constitutes the proinflammatory subpopulation was obtained by demonstrating coexpression of FR-\u03b2 with other proinflammatory markers, including CCR2 and HLA-DR. Synovial monocytes from the joints of patients with RA were also shown to express FR-\u03b2. As inhibition of the chemotaxis of proinflammatory monocytes into sites of inflammation has been explored frequently as a means of controlling autoimmune diseases, demonstration that FR-\u03b2 is uniquely expressed on this proinflammatory subpopulation offers a new strategy to suppress migration of inflammatory monocytes into sites of inflammation.<\/jats:p>","DOI":"10.1189\/jlb.2ab0713-372r","type":"journal-article","created":{"date-parts":[[2014,7,12]],"date-time":"2014-07-12T06:09:40Z","timestamp":1405145380000},"page":"563-570","source":"Crossref","is-referenced-by-count":50,"title":["Folate receptor-\u03b2 constitutes a marker for human proinflammatory monocytes"],"prefix":"10.1093","volume":"96","author":[{"given":"Jiayin","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Chemistry, Purdue University , West Lafayette, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R","family":"Hilgenbrink","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Purdue University , West Lafayette, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Purdue University , West Lafayette, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Feng","sequence":"additional","affiliation":[{"name":"Protein Interactions Group, Center for Cancer and Inflammation Program, National Cancer Institute-Frederick, National Institutes of Health , Frederick, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimiter S","family":"Dimitrov","sequence":"additional","affiliation":[{"name":"Protein Interactions Group, Center for Cancer and Inflammation Program, National Cancer Institute-Frederick, National Institutes of Health , Frederick, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael B","family":"Lockwood","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Clarian Arnett Health , Lafayette, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J","family":"Amato","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Memorial Hermann Cancer Center, Division of Oncology, University of Texas Health Science Center at Houston, Medical School , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip S","family":"Low","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Purdue University , West Lafayette, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,7,11]]},"reference":[{"key":"2023032708374840300_","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/nri3073","article-title":"Protective and pathogenic functions of macrophage subsets","volume":"11","author":"Murray","year":"2011","journal-title":"Nat. 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